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草业学报 ›› 2017, Vol. 26 ›› Issue (6): 120-130.DOI: 10.11686/cyxb2016507

• 研究论文 • 上一篇    下一篇

玄参与烟草间作对土壤细菌群落结构的影响

张东艳1,2, 王军2, 杨水平1,*, 张雪3,*, 刘京2, 赵建2, 何大敏1, 杨红军1, 莫静静2, 苟剑渝2, 赵新梅1, 蒋卫2, 丁伟1, 陈大霞3   

  1. 1.西南大学资源环境学院,重庆400715;
    2.贵州省烟草公司遵义市公司,贵州 遵义563000;
    3.重庆市中药研究院,重庆400065
  • 收稿日期:2016-12-30 修回日期:2017-03-14 出版日期:2017-06-20 发布日期:2017-06-20
  • 通讯作者: E-mail:yang-sp@163.com
  • 作者简介:张东艳(1991-),女,河南商丘人,在读硕士。E-mail:1750401841@qq.com
  • 基金资助:
    贵州省烟草公司遵义市公司研究开发项目(201404),国家烟草专卖局重点项目(NY20150601070012),中央高校基本科研业务费专项资金(SWU113094),国家自然科学基金(31370602)和重庆市科委“科技平台与基地建设”项目(CSTC,2014ptyjd10001)资助

Influence of Scrophularia ningpoensis-tobacco intercropping on bacterial community structure in soil

ZHANG Dong-Yan1,2, WANG Jun2, YANG Shui-Ping1,*, ZHANG Xue3,*, LIU Jing2, ZHAO Jian2, HE Da-Min1, YANG Hong-Jun1, MO Jing-Jing2, GOU Jian-Yu2, ZHAO Xin-Mei1, JIANG Wei2, DING Wei1, CHEN Da-Xia3   

  1. 1.College of Resources and Environment, Southwest University, Chongqing 400715, China;
    2.Zunyi Subsidiary of Guizhou Tobacco Company, Zunyi 563000, China;
    3.Chongqing Academy of Chinese Materia Medica, Chongqing 400065,China
  • Received:2016-12-30 Revised:2017-03-14 Online:2017-06-20 Published:2017-06-20

摘要: 为研究玄参与烟草不同种植模式下对土壤细菌群落结构的影响,采用Illumina MiSeq技术测定土壤细菌,结果表明,玄参土壤细菌的丰富度和多样性高于植烟土壤,但未达显著水平,间作有降低植烟土壤细菌丰富度和多样性而提高玄参土壤细菌多样性的趋势。作物和种植模式在门和属水平上都影响着细菌丰度及优势菌排序,门水平上,植烟土壤中 Saccharibacteria显著高于玄参土壤,Gemmatimonadetes在玄参土壤中显著高于植烟土壤,Bacteroidetes在不同种植模式间差异显著;属水平上unclassified Xanthomonadaceae, norank Saccharibacteria, uncultured Gaiellales, norank SC-I-84等植烟土壤中显著高于玄参土壤,norank GR-WP33-30, norank JG30a-KF-32, Gemmatimonas, Candidatus_Solibacter, uncultured Nitrosomonadaceae, norank C0119, norank Subgroup_6, norank DA101_soil_group、norank BIrii41, Anaeromyxobacter, unclassified Xanthomonadales, uncultured Methylophilaceae, norank JG37-AG-20等玄参土壤中显著高于植烟土壤,而几乎没有菌属受种植模式影响达显著水平。主成分分析也表明作物较种植模式影响更大。因此,土壤细菌群落结构均受作物和种植模式影响,但作物影响更大,存在明显作物效应;间作土壤因作物而具有微生物群落二元结构。

Abstract: To explore the influence of Scrophularia ningpoensis and tobacco planting on bacterial community structure in soil under different planting patterns, we used Illumina MiSeq technology to identify bacteria in soil. It was found that the richness and diversity of bacterial species in S. ningpoensis soil tended to be higher than in tobacco soil, but differences were not statistically significant. Crop species and planting pattern both affected the abundance and the order of dominant bacteria. At phylum level, presence of Saccharibacteria in tobacco soil was significantly higher than that in S. ningpoensis soil, whereas presence of Gemmatimonadetes in S. ningpoensis soil was significantly higher than that in tobacco soil. Bacteroidetes occurrence also varied between different planting patterns. At genus level, taxa significantly more common in tobacco soil than in S. ningpoensis soil, included unclassified Xanthomonadaceae, norank Saccharibacteria, uncultured Gaiellales, and norank SC-I-84. Meanwhile, for S. ningpoensis soil, significantly more common taxa (than in tobacco soil) included norank GR-WP33-30, norank JG30a-KF-32, Gemmatimonas, Candidatus_Solibacter, uncultured Nitrosomonadaceae, norank C0119, norank Subgroup_6, norank DA101_soil_group, norank BIrii41, Anaeromyxobacter, unclassified Xanthomonadales, uncultured Methylophilaceae, and norank JG37-AG-20. Planting pattern had almost no effect on genera detected. Principal component analysis also showed that crops had a greater influence than planting patterns. In summary, soil bacteria community structure differed between tobacco and S. ningpoensis crops, and was also affected to a lesser extent by planting pattern. Hence, in an intercropped field the microbial community displays spatial heterogeneity determined by the crop species.