锰胁迫对苍耳种子萌发及幼苗生理生化特性的影响

doi:10.11686/cyxb2017033

摘要/Abstract

摘要： 为探明苍耳对锰胁迫适应的生理生化机制,以苍耳种子为试验材料,采用培养皿滤纸法,研究了不同浓度锰(0,1000,5000,10000,15000和20000 μmol/L)胁迫对苍耳种子萌发、幼苗生长及生理生化特性的影响。结果表明:1)1000~5000 μmol/L锰胁迫显著提高了苍耳种子的萌发率,增加了幼苗的芽长和根系活力;当浓度>5000 μmol/L时,发芽势、发芽指数、活力指数以及根长、鲜重、干重、根冠比均显著降低;2)随着锰浓度的升高,苍耳幼苗叶片中可溶性糖、可溶性蛋白和脯氨酸含量以及超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、愈创木酚过氧化物酶(GPX)、过氧化氢酶(CAT)活性均呈现先增加后下降的变化趋势;3)苍耳幼苗体内活性氧和膜脂过氧化物含量随着锰浓度的升高显著增加,超氧阴离子自由基(O2· -)产生速率、羟自由基(OH^·)浓度、H₂O₂和MDA含量分别增加了154.69%、47.29%、100.09%和200.96%。研究结果显示,苍耳对锰胁迫具有较强的耐性,可作为锰矿废弃地修复的备选植物。

Abstract: To investigate the physiological and biochemical adaption mechanisms of Xanthium sibiricum to manganese (Mn) stress, Xanthium sibiricum seeds were treated with different concentrations of Mn (0, 1000, 5000, 10000, 15000 and 20000 μmol/L) and subsequently germinated on filter paper. Seed germination, seedling growth, physiological and biochemical characteristics of seedlings were examined. 1) Compared with the control, germination rate, bud length and root vigor increased significantly at 1000-5000 μmol/L Mn. Germination potential, germination index, vigor index, root length, fresh weight, dry weight and root-shoot ratio significantly decreased with Mn concentrations over 5000 μmol/L. 2) Soluble sugar, soluble protein, proline and superoxide dismutase (SOD), ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and catalase (CAT) initially increased and then decreased with the rising Mn concentration. 3) Reactive oxygen species (ROS) and malondialdehyde (MDA) in seedlings increased significantly with the increase in Mn concentration. The O2· - (superoxide anion radical), OH^· (hydroxyl radical), H₂O₂ and MDA content increased by 154.69%, 47.29%, 100.09% and 200.96% at the highest Mn concentration, respectively. It showed that X. sibiricum has strong Mn tolerance and adaptability, and is potentially utilizable for phytoremediation.

潘高, 张合平, 刘鹏, 刘文胜. 锰胁迫对苍耳种子萌发及幼苗生理生化特性的影响[J]. 草业学报, 2017, 26(11): 157-166.

PAN Gao, ZHANG He-Ping, LIU Peng, LIU Wen-Sheng. Effect of manganese stress on seed germination, seedling physiological and biochemical characteristics of Xanthium sibiricum[J]. Acta Prataculturae Sinica, 2017, 26(11): 157-166.

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