苜蓿DUS测试标准品种SSR分子标记指纹图谱的构建

doi:10.11686/cyxb2017038

草业学报 ›› 2017, Vol. 26 ›› Issue (11): 47-56.DOI: 10.11686/cyxb2017038

苜蓿DUS测试标准品种SSR分子标记指纹图谱的构建

闵学阳¹, 刘文献^1,*, 张正社¹, 韦兴燚¹, 齐晓², 张义³, 刘志鹏¹, 王彦荣^1,*

1.草地农业生态系统国家重点实验室,农业部草牧业创新重点实验室,兰州大学草地农业科技学院,甘肃兰州 730020;
2.全国畜牧总站,全国草品种审定委员会办公室,北京 100125;
3.全国畜牧总站,北京100193

收稿日期:2017-02-14 修回日期:2017-04-10 出版日期:2017-11-20 发布日期:2017-11-20
通讯作者: *E-mail: liuwx@lzu.edu.cn, yrwang@lzu.edu.cn
作者简介:闵学阳(1991-),男,甘肃张掖人,在读硕士。E-mail: minxy15@lzu.edu.cn
基金资助:
国家自然科学基金项目(31502000),教育部创新团队(IRT_17R50),高等学校学科创新引智计划(B12002),国家草品种区域试验项目(21301060001)和兰州大学中央高校基本科研业务费专项资金(lzujbky-2016-8)资助

Construction of SSR marker fingerprint database of standard alfalfa varieties utilizing DUS tests

MIN Xue-Yang¹, LIU Wen-Xian^1,*, ZHANG Zheng-She¹, WEI Xing-Yi¹, QI Xiao², ZHANG Yi³, LIU Zhi-Peng¹, WANG Yan-Rong^1,*

1.State Key Laboratory of Grassland Argo-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
2.National Animal Husbandry Service, Office of the Chinese Herbage Cultivar Registration Board, Beijing 100125, China;
3.National Animal Husbandry Service, Beijing 100193, China

Received:2017-02-14 Revised:2017-04-10 Online:2017-11-20 Published:2017-11-20

摘要/Abstract

摘要： 苜蓿在育种过程中,由于其异花授粉特性和骨干亲本的集中使用,导致品种间的遗传差异日益缩小,品种之间通过形态学鉴定愈加困难。通过构建苜蓿DUS测试标准品种DNA指纹图谱数据库,可以实现苜蓿品种的快速、准确鉴定。本研究利用筛选得到的10个SSR标记对33个苜蓿DUS测试标准品种进行指纹图谱构建和遗传多样性分析。结果表明,10对SSR引物共扩增出69个等位基因,平均每个标记可产生6.9个等位基因;多态性比率(PPB)从25%到90%不等,平均值为56.7%;各SSR标记的多态信息含量(PIC)范围为0.56~0.87,平均值为0.75。利用不同引物间的组合能有效区分33个苜蓿 DUS标准品种,并为每份标准品种建立了唯一标识的指纹代码,为苜蓿品种的审定和保护以及遗传背景分析提供了技术依据。

Abstract: Due to cross-pollinating characteristic and the excessive use of backbone materials during the alfalfa breeding, the genetic diversity of alfalfa varieties is declining, and the discrimination of different varieties through phenotypical characteristics is becoming more difficult. The construction of a DNA fingerprint database of standard alfalfa varieties through the distinctness, uniformity and stability (DUS) test can facilitate the identification of alfalfa varieties. In this study we analyzed the genetic diversity among 33 standard alfalfa varieties with a DUS test and the DNA fingerprint of each variety was constructed based on 10 SSR markers. The results showed that a total of 69 alleles were generated from the 10 SSR markers, with an average of 6.9 alleles per marker; the polymorphic ratio varied from 25% to 90%, with an average of 56.7%. The polymorphic information content per SSR marker varied from 0.56~0.87, with an average of 0.75. The 33 varieties could be clearly distinguished by the 10 SSR markers. The DNA fingerprint of each variety was established providing a technical basis for the validation and conservation of alfalfa varieties and genetic background analysis.

闵学阳, 刘文献, 张正社, 韦兴燚, 齐晓, 张义, 刘志鹏, 王彦荣. 苜蓿DUS测试标准品种SSR分子标记指纹图谱的构建[J]. 草业学报, 2017, 26(11): 47-56.

MIN Xue-Yang, LIU Wen-Xian, ZHANG Zheng-She, WEI Xing-Yi, QI Xiao, ZHANG Yi, LIU Zhi-Peng, WANG Yan-Rong. Construction of SSR marker fingerprint database of standard alfalfa varieties utilizing DUS tests[J]. Acta Prataculturae Sinica, 2017, 26(11): 47-56.

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苜蓿DUS测试标准品种SSR分子标记指纹图谱的构建

Construction of SSR marker fingerprint database of standard alfalfa varieties utilizing DUS tests

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