沟叶结缕草八氢番茄红素基因ZmPSY的克隆、亚细胞定位及表达分析

doi:10.11686/cyxb2017176

草业学报 ›› 2017, Vol. 26 ›› Issue (11): 69-76.DOI: 10.11686/cyxb2017176

沟叶结缕草八氢番茄红素基因ZmPSY的克隆、亚细胞定位及表达分析

董笛^1**, 滕珂^2**, 于安东¹, 檀鹏辉¹, 梁小红^1,*, 韩烈保^1,*

1.北京林业大学草坪研究所,北京100083;
2.北京草业与环境研究发展中心,北京100097

收稿日期:2017-04-06 修回日期:2017-05-12 出版日期:2017-11-20 发布日期:2017-11-20
通讯作者: *E-mail: liang81511@163.com, hanliebao@163.com
作者简介:董笛(1994-),女,山东青岛人,在读硕士。E-mail: didoscori@163.com。滕珂(1989-),男,山东淄博人,在读博士后。E-mail: tengke.123@163.com。**共同第一作者
基金资助:
北京市博后基金“苔草优良耐荫基因的挖掘与分子调控机制研究(No.2017-ZZ-087)”,北京市农林科学院博后基金,深圳市科技计划项目(JCYJ20160331151245672,JSGG20160229155434792)和国家高技术研究发展计划(863计划)(2013AA102607)资助

Cloning, subcellular localization and expression analysis of a novel phytoene synthase gene, ZmPSY, in Zoysia matrella

DONG Di^1**, TENG Ke^2**, YU An-Dong¹, TAN Peng-Hui¹, LIANG Xiao-Hong^1,*, HAN Lie-Bao^1,*

1.Turfgrass Research Institute of Beijing Forestry University, Beijing 100083, China;
2.Beijing Research & Development Center for Grass and Environment, Beijing 100097, China

Received:2017-04-06 Revised:2017-05-12 Online:2017-11-20 Published:2017-11-20
About author:**These authors contributed equally to this work.

摘要/Abstract

摘要： 八氢番茄红素合成酶(phytoene synthase,PSY)是生物类胡萝卜素合成途径中的一个关键酶。本实验从沟叶结缕草中克隆得到ZmPSY基因(Genbank登录号为:KY264128)。该基因开放阅读框为1230 bp,编码409个氨基酸残基。亚细胞定位结果显示,ZmPSY定位于叶绿体中。实时荧光定量分析表明,ZmPSY基因在沟叶结缕草幼嫩的叶片中表达量最高。ZmPSY可受外施激素脱落酸(ABA)的诱导,但受外施激素水杨酸(SA)、茉莉酸甲酯(MeJA)的抑制。本实验为深入研究沟叶结缕草八氢番茄红素合成酶基因的功能奠定了基础。

Abstract: The formation of phytoene by phytoene synthase is a key limiting step in the carotenoid biosynthesis pathway. In this study a novel gene, ZmPSY (GenBank Accession number: KY264128), was cloned from Zoysia matrella. Subcellular localization results proved that ZmPSY was localized in the chloroplasts. Expression analysis showed that ZmPSY had a higher expression level in leaves compared to roots and stems. The expression of ZmPSY in leaves also varied at different development stages, with the greatest expression seen in young leaves. qRT-PCR determination revealed that ZmPSY could be induced by exogenous abscisic acid (ABA), but suppressed by salicylic acid (SA) and methyl jasmonate (MeJA). This study paved the way for further discovery of the functional roles of ZmPSY.

董笛, 滕珂, 于安东, 檀鹏辉, 梁小红, 韩烈保. 沟叶结缕草八氢番茄红素基因ZmPSY的克隆、亚细胞定位及表达分析[J]. 草业学报, 2017, 26(11): 69-76.

DONG Di, TENG Ke, YU An-Dong, TAN Peng-Hui, LIANG Xiao-Hong, HAN Lie-Bao. Cloning, subcellular localization and expression analysis of a novel phytoene synthase gene, ZmPSY, in Zoysia matrella[J]. Acta Prataculturae Sinica, 2017, 26(11): 69-76.

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沟叶结缕草八氢番茄红素基因ZmPSY的克隆、亚细胞定位及表达分析

Cloning, subcellular localization and expression analysis of a novel phytoene synthase gene, ZmPSY, in Zoysia matrella

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