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草业学报 ›› 2009, Vol. 18 ›› Issue (2): 95-101.

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苍耳对不同植物幼苗的化感作用研究

高兴祥1,李美1*,高宗军1,张宏军2,张佳2   

  1. 1.山东省农业科学院植物保护研究所, 山东 济南 250100;
    2.农业部农药检定所, 北京 100026
  • 收稿日期:2008-04-07 出版日期:2009-04-20 发布日期:2009-04-20
  • 作者简介:高兴祥(1977-),男,山东沂水人,助理研究员,硕士。E-mail:xingxiang02@163.com
  • 基金资助:
    国家863计划资助项目(2006AA10A214),山东省农业科学院创新基金(2006YCX018),山东省自然基金(Q2004D02)和山东省农业科学院青年科学基金(2005YQ025)资助。

Allelopathic potential of Xanthium sibiricum on seeds germination and seedling growth of different plants

GAO Xing-xiang1, LI Mei1, GAO Zong-jun1, ZHANG Hong-jun2, ZHANG Jia2   

  1. 1.Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China;

    2.Institute for the Control of Agrochemicals, Ministry of
    Agriculture, Beijing 100026, China
  • Received:2008-04-07 Online:2009-04-20 Published:2009-04-20

摘要: 在室内采用离体生物测定法,测定了苍耳水浸提物对小麦、高粱、黄瓜、油菜和萝卜的化感效应。结果表明,苍耳水浸提液对5种植物种子的萌发和生长均有较强的抑制作用,且相同浓度处理时,对胚根的生长抑制作用高于对胚芽的抑制作用。5种受体植物中,以油菜最为敏感,其次为萝卜。本研究还通过测定化感物质对植物光合作用的影响及MDA含量的变化,初步探讨了苍耳化感物质的作用特征。在0.025 g/mL浓度下,苍耳对去胚乳小麦幼芽、幼根的抑制率分别为58.33%和67.31%;同样浓度下,苍耳对黑暗条件下培养的高粱幼芽、幼根的抑制率分别为5.58%和32.34%;可以看出,苍耳对植物光合作用的影响明显高于对非光合作用的影响。化感物质处理后黄瓜和萝卜幼苗MDA含量均高于未加化感物质的对照处理,且化感物质浓度越高MDA含量越高。由此可以看出,苍耳对植物的化感作用与抑制植物的光合作用并使植物的MDA含量升高有关,对植物的非光合作用亦有不同程度的影响。

Abstract: Allelopathic effects of water extract from Xanthium sibiricum were tested with a petri dish cultivation method. The extract strongly inhibited germination, and seedling growth of sorghum(Sorghum vulgare), cucumber (Cucumis sativus), wheat(Triticum aestivum), rape(Brassica campestris), and radish (Raphanus sativus). The inhibition of rape was the strongest followed by radish. Roots were inhibited more strongly than shoots at the same concentrations of the extract. Allelopathic effects of extract on photosynthesis activity and malondialdehyde (MDA) content were also tested. The inhibitions of X. sibiricum extract to embryo and root of endosperm-removed wheat were 58.33%, 67.31% at 0.025 0 g/mL respectively, while they were 5.58%, 32.34% to sorghum cultivated in the dark. The MDA contents of cucumber and radish increased after treatment with X. sibiricum extract. These Results indicate that the allelopathic effects of X. sibiricum could inhibit photosynthesis activity and MDA, and could also inhibit non-photosynthesis activity to some extent. 

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