Abstract: Using sequence-related amplified polymorphism (SRAP) molecular markers, 70 samples containing 12 annual Lolium multiflorum hybrid combinations and their progenies were analyzed to study polymorphism and to detect true or false hybrid seeds. 1) Ten primer pairs produced 101 polymorphic bands, averaging 10.1 bands per primer pair. The average percentage of polymorphic bands was 77.1%. Samples had relatively high molecular genetic polymorphisms so it was feasible to identify the parents and hybrids. 2) Electrophoretic bands of the 62 hybrids identified by SRAP were consistent with results of different hybrid combinations. Progenies with the marker bands of the male but not the female parents were found in 48 progeny which were therefore true hybrids.