重楼属9种5变种花粉活力与柱头可授性特性研究

草业学报 ›› 2009, Vol. 18 ›› Issue (4): 61-66.

重楼属9种5变种花粉活力与柱头可授性特性研究

丁春邦¹,李强¹,李燕¹,张利¹,杨瑞武¹,周永红²

1.四川农业大学生命科学与理学院,四川雅安 625014;
2.四川农业大学作物基因资源
与遗传改良教育部重点实验室,四川雅安 625014

收稿日期:2008-09-08 出版日期:2009-08-20 发布日期:2009-08-20
作者简介:丁春邦(1966-),女,四川富顺人,教授,博士。E-mail:dcb@sicau.edu.cn
基金资助:
教育部长江学者和创新团队发展计划(IRT0453)和四川省科技厅项目(2008JY0094-2)资助。

Pollen viability and stigma receptivity of nine species and five variety in Paris

DING Chun-bang¹, LI Qiang¹, LI Yan¹, ZHANG Li¹, YANG Rui-wu¹, ZHOU Yong-hong²

1.College of Biology and Science, Sichuan Agricultural University, Ya’an 625014, China;
2.Key Laboratory of Crop Genetic Resources and Improvement, Ministry of
Education, Sichuan Agricultural University, Ya’an 625014, China

Received:2008-09-08 Online:2009-08-20 Published:2009-08-20

摘要/Abstract

摘要： 用TTC法测定重楼属9种5变种花粉活力,用联苯胺-过氧化氢法测定其柱头的可授性。结果表明,1)重楼属植物在自然条件下的花粉活力均较低,最高的为凌云重楼,可达52.8%,其余种类都在50%以下;2)不同种类之间、同种不同变种以及同种不同居群之间花粉的活力差异较大;3)不同种类重楼的柱头可授性差异较大,但同种不同居群间柱头可授性比较一致;4)黑籽重楼和巴山重楼的柱头一直没有可授性,五指莲、金线重楼、多叶重楼、长柱重楼、卵叶重楼、七叶一枝花和狭叶重楼的柱头可授性与花粉活力呈正相关,而凌云重楼、卷瓣重楼、短瓣球药隔重楼和滇重楼却没有这种关联性;5)在整个开花传粉过程中,重楼的花药每天都重复着上午开裂、傍晚关闭的变化。

Abstract: Pollen viabilities of nine species and five varieties of Paris were evaluated using TTC(2, 3, 5-triphenyl tetrazolium chloride), while stigma receptivities were measured by a benzidine-H₂O₂ method. 1) Pollen viabilities of Paris species were lower than 50%, except for P. cronuistii, which was up to 52.8%; 2) Pollen viabilities were different between species, varieties, and populations; 3) Stigma receptivities were different between species, but the same among populations within a species; 4) Stigma of P. thibetica and P. bashanensis had no receptivity at all. Stigma receptivities were in positively related to pollen viabilities of P. axialis, P. delavayi, P. polyphylla, P. forrestii, P. delavayi var. petiolata, P. polyphylla var. chinensis and P. polyphylla var. stenophylla. However, there were no correlations in P. cronuistii, P. polyphylla var. yunnanensis, P. undulata and P. fargesii var. brevipetalata; 5) Anthers opened in the morning and closed at dusk each day during the blooming period.

中图分类号:

Q944.42

丁春邦,李强,李燕,张利,杨瑞武,周永红. 重楼属9种5变种花粉活力与柱头可授性特性研究[J]. 草业学报, 2009, 18(4): 61-66.

DING Chun-bang, LI Qiang, LI Yan, ZHANG Li, YANG Rui-wu, ZHOU Yong-hong. Pollen viability and stigma receptivity of nine species and five variety in Paris[J]. Acta Prataculturae Sinica, 2009, 18(4): 61-66.

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