几种禾本科牧草内生细菌的分布特性

草业学报 ›› 2010, Vol. 19 ›› Issue (3): 124-131.

几种禾本科牧草内生细菌的分布特性

车建美¹,刘波^1*,张彦¹,胡桂萍¹,黄勤楼²,陈忠钿²,翁伯琦^2*

1.福建省农业科学院农业生物资源研究所,福建福州350003;
2.福建省农业科学院农业生态研究所,福建福州 350013

收稿日期:2009-09-01 出版日期:2010-03-25 发布日期:2010-06-20
作者简介:车建美(1977-),女,山东乳山人,助理研究员,在读博士。E-mail:chejm2002@163.com
基金资助:
“十一五”国家科技支撑计划(2007BAD89B13-8)和福建省财政专项-福建省农业科学院科技创新团队建设基金(STIF-Y03)资助

Distribution characteristics of endophytic bacteria isolated from forage grasses

CHE Jian-mei¹, LIU Bo¹, ZHANG Yan¹, HU Gui-ping¹, HUANG Qin-lou², CHEN Zhong-dian², WENG Bo-qi²

1.Agricultural Bioresource Research Institute, Fujian Academy of Agricultural Sciences,
Fuzhou 350003, China;
2.Research Center of Ecological Agriculture, Fujian
Academy of Agricultural Sciences, Fuzhou 350013, China

Received:2009-09-01 Online:2010-03-25 Published:2010-06-20

摘要/Abstract

摘要： 不同禾本科牧草植株内生细菌含量差异显著,杂交狼尾草闽牧六号内生细菌含量最高,达26.800×10⁴CFU/g。具体到各个部位,禾本科牧草不同部位内生细菌含量差异也显著。通过微生物鉴定系统对内生菌进行鉴定的结果表明,64株内生细菌中与MIDI数据库比较相似性指数大于0.500,可确定其菌种名称的有50株,属于19个种,隶属于12个属。以分离到的19种内生菌为样本,以各菌株在各部位出现与否为指标,用类平均法对数据进行系统聚类分析,当λ=8.35时,可将19种内生细菌分为4个大类群。从聚类结果显示,内生菌与宿主植株和不同分离部位都存在相关性,与宿主植株的关系更密切。

Abstract: The endophytic bacteria were isolated from different forage grasses according to culture method and identified by using fatty-acid identification technology. The results showed that the contents of endophytic bacteria of hybrid pennisetum “Minmu six”, which was 26.800×10⁴ CFU/g, was higher than other forage grasses. The endophytic bacteria isolated from different forage grasses were from 19 species belonging to 12 genera. The cluster analysis results showed that the 19 strains could be separated into 4 groups. It was concluded that the distribution characterics of endophytic baceria were related to different forage grasses and different isolated parts, especially to the different forage grasses.

中图分类号:

车建美,刘波,张彦,胡桂萍,黄勤楼,陈忠钿,翁伯琦. 几种禾本科牧草内生细菌的分布特性[J]. 草业学报, 2010, 19(3): 124-131.

CHE Jian-mei, LIU Bo, ZHANG Yan, HU Gui-ping, HUANG Qin-lou, CHEN Zhong-dian, WENG Bo-qi. Distribution characteristics of endophytic bacteria isolated from forage grasses[J]. Acta Prataculturae Sinica, 2010, 19(3): 124-131.

参考文献

[1] 刘蕴哲, 何劲, 张杰, 等. 植物内生真菌及其活性代谢产物研究进展[J]. 菌物研究, 2005, 3(4): 30-36.
[2] Vogl A E. Mehl und die anderen Mehlprodukte der Cerealien und Leguminosen. Z.[J]. Nahrungs mittel-Untersuchung, Hyg. Warenkunde, 1898, 12: 25-29.
[3] 何劲, 刘蕴哲, 康冀川. 植物内生菌及其在农业和医学上的用途[J]. 贵州农业科学, 2006, 34(3): 113-115.
[4] 姜怡, 杨颖, 陈华红, 等. 植物内生菌资源[J]. 微生物学通报, 2005, 32(6): 146-147.
[5] Kobayashi D Y, Palumbo J D. Bacterial endophytes and their effects on plants and uses in agriculture[A]. In: Bacon C W, White J F. Microbial Endophytes[M]. New York: Jr. Marcel Dekker, Inc., 2000: 199-237
[6] 王刚, 李志强. 小麦内生细菌的分离及其对小麦纹枯病的拮抗作用[J]. 微生物通报, 2005, 32(2): 20-24.
[7] 罗明, 芦云, 张祥林. 棉花内生细菌的分离及生防益菌的筛选[J]. 新疆农业科学, 2004, 41(5): 277-282.
[8] 宋子红, 丁立孝, 马伯军, 等. 花生内生菌的种群及动态分析[J]. 植物保护学报, 1999, 26(4): 309-314.
[9] 周婧, 高增贵, 何秀玲, 等. 甜瓜枯萎病拮抗内生细菌筛选研究[J]. 北方园艺, 2008, (2): 225-227.
[10] Fernando E V, Monica P R, Francisco P, et al. Endophytic bateria in Coffea arabica L.[J]. Journal of Basic Microbiology, 2005, 45(5): 371-380.
[11] 付业勤, 蔡吉苗, 刘先宝, 等. 香蕉内生细菌分离、活性评价及数量分布[J]. 热带作物学报, 2007, 28(4): 78-83.
[12] Angela S, Birgit R, Gabriele B. Endophytic bacterial communities of field-grown potato plants and their plant-growth-promoting and antagonistic abilities[J]. Microbiology, 2004, 50: 239-249.
[13] 彭细桥, 周国生, 邓正平, 等. 烟草青枯病内生拮抗菌的筛选、鉴定及其防效测定[J]. 植物病理学报, 2007, 37(6): 670-674.
[14] 孔庆科, 丁爱云. 内生细菌作为生防因子的研究进展[J]. 山东农业大学学报(自然科学版), 2001, 32(2): 256-260.
[15] 李志真. 杨梅根瘤内生菌的生物学特性[J]. 林业科学, 2009, 45(1): 81-87.
[16] 史应武, 娄恺, 李春. 天山北坡甜菜内生菌分离鉴定及其动态变化[J]. 生态学报, 2009, 29(5): 2375-2382.
[17] Berg G, Krechel A, Ditz M, et al. Endophytic and ectophytic potato-associated bacterial communities differ in structure and antagonistic function against plant pathogenic fungi[J]. FEMS Microbiology Ecology, 2005, 51(2): 215-229.
[18] 黄贵修, Yuka T, Segenet K. 臂形草内生菌特异DNA片段的克隆及其分子鉴定[J]. 热带作物学报, 2002, 23(4): 58-61.
[19] Ahlholm J U, Helander M, Henriksson J, et al. Environmental conditions and host genotype direct genetic diversity of venturia ditricha, a fungal endophyte of birch trees[J]. Evolution, 2002, 56(8): 1566-1573.
[20] 谢永芳, 梁亦龙, 王会会, 等. 大蒜内生菌抑菌蛋白提取研究[J]. 江苏农业科学, 2009, (1): 122-123.
[21] Sturz A V, Christie B R, Nowak J. Baterial endophytes: Potential role in developing sustainable systems of crop production[J]. Critical Reviews in Plant Sciences, 2000, 19(1): 1-30.
[22] 黄东益, 黄小龙, Segenet K. 旗草内生真菌与旗草抗病性研究[J]. 草业学报, 2009, 18(2): 39-45.
[23] 黄东益, 黄小龙. 禾本科牧草内生真菌的研究与应用[J]. 草业学报, 2008, 17(3): 128-136.
[24] Siciliano S D, Germida J J. Taxonomic diversity of bacteria associated with the roots of field-grown transgenic Brassica napus cv. Quest, compared to the non-transgenic B. napus cv. Excel and B. rapus cv. Parkland[J]. FEMS Microbiology Ecology, 1999, 29(3): 263-272.
[25] 李倍金, 罗明, 周俊, 等. 几种禾草内生固氮菌的分离及固氮活性测定[J]. 草业学报, 2008, 17(5): 37-42.
[26] Clay K, Holah J. Fungal endophyte symbiosis and plant diversity in successional fields[J]. Science, 1999, 285: 1742-1745.
[27] Peters S, Draeger S, Aust H J, et al. Interactions in dual cultures of endophytic fungi with host and nonhost plant calli[J]. Mycologia, 1998, 90: 360-367.
[28] 刘志辉, 蔡杏姗, 竺澎波, 等. 应用气相色谱技术分析全细胞脂肪酸快速鉴定分枝杆菌[J]. 中华结核和呼吸杂志, 2005, 28(6): 403-406.
[29] 张国赏, 吴文娟, 潘仁瑞. 气相色谱-质谱法检测细胞脂肪酸及其在细菌鉴定上的应用[J]. 合肥联合大学学报, 2000, 10(4): 92-96.
[30] Riley M S, Cooper V S, Lenski R E, et al. Rapid phenotypic change and diversification of a soil bacterium during 1000 generations of experimental evolution[J]. Microbiology, 2001, 147: 995-1006.
[31] Ozbek A, Aktas O. Identification of three strains of Mycobacterium species isolated from clinical samples using fatty acid methyl ester profiling[J]. The Journal of International Medical Research, 2003, 31(2): 133-140.
[32] Muller K, Schmid E N, Kroppenstedt R M. Improved identification of mycobacteria by using the microbial identification system in combination with additional trimethylsulfonium hydroxide pyrolysis[J]. Journal of Clinic Microbiology, 1998, 36(9): 2477-2480.
[33] Basile F, Voorhces K J, Hadtleld T L. Microorganism gram-type differentiation based on pyrolysis mass spectrometry of bacterial fatty acid methyl ester extracts[J]. Applied and Environmental Microbiology, 1995, 61(4): 1534-1539.
[34] 王瑶瑶, 韩烈保, 曾会明. 禾本科植物内生菌研究进展[J]. 生物技术通报, 2008, (3): 33-38.
[35] 韦红群, 邓建珍, 曹建华, 等. 柱花草根系与根际微生物类群的研究[J]. 草业科学, 2009, 26(1): 69-73.
[36] 朱育菁, 陈璐, 蓝江林, 等. 茶叶内生菌的分离鉴定及其生防功能初探[J]. 福建农林大学学报(自然科学版), 2009, 38(2): 129-134.
[37] 朱育菁, 王秋红, 陈璐, 等. 龙眼内生菌的分离与脂肪酸鉴定[J]. 亚热带植物科学, 2008, 37(4): 22-25.
[38] Cabral C M, Cherqui A, Pereira A, et al. Purification and characterization of two distinct metalloproteases secreted by the entomopathogenic bacterium photorhabdus sp. strain Az29[J]. Applied and Environmental Microbiology, 2004, 70: 3831-3838.
[39] Mariёt J V D W, Henk J S, Jan A M B. Rhodococcus erythropolis DCL14 contains a novel degradation pathway for limonene[J]. Applied and Environmental Microbiology, 1999, 65(5): 2092-2102.
[40] Bernardo V R, Jacquie M M, Robert A C, et al. Susceptibility of calves to challenge with Salmonella typhimurium 4/74 and derivatives harbouring mutations in htrA or purE[J]. Microbiology, 2000, 146(11): 2775-2783.
[41] Wolf A, Fritze A, Hagemann M, et al. Stenotrophomonas rhizophila sp. nov., a novel plant-associated bacterium with antifungal properties[J]. International Journal of Systematic and Evolutionary Microbiology, 2002, 52: 1937-1944.
[42] Fisher P J, Petrini O, Scott H M. The distribution of some fungal and bacterial endophytes in maize (Zea mays L.)[J]. New Phyto1ogist, 1992, 122: 299-305.
[43] 马同锁, 田苗珍, 袁红楼, 等. 不同生长环境对2种蔬菜内生菌分布的影响[J]. 安徽农业科学, 2009, 37(17): 7812-7813, 7815.