野生狗牙根种质资源的AFLP遗传多样性分析

草业学报 ›› 2010, Vol. 19 ›› Issue (3): 155-161.

野生狗牙根种质资源的AFLP遗传多样性分析

齐晓芳,张新全^*,凌瑶,陈仕勇,刘伟

四川农业大学草业科学系,四川雅安 625014

收稿日期:2009-08-10 出版日期:2010-03-25 发布日期:2010-06-20
作者简介:齐晓芳(1984-),女,山西寿阳人,在读硕士。E-mail:qixf66@163.com
基金资助:
科技部973项目(编号2007CB108907)和教育部新世纪优秀人才支持计划(编号NCET-04-0909)资助。

Genetic diversity of wild Cynodon dactylon germplasm detected by AFLP markers

QI Xiao-fang, ZHANG Xin-quan, LING Yao, CHEN Shi-yong, LIU Wei

Department of Grassland, Sichuan Agricultural University, Ya’an 625014, China

Received:2009-08-10 Online:2010-03-25 Published:2010-06-20

摘要/Abstract

摘要： 利用AFLP标记对采自非洲以及中国四川、重庆、云南、贵州和西藏五省区的共44份野生狗牙根材料进行遗传多样性分析。10对引物共扩增出462个条带,其中多态性条带有452条,多态性条带比率为97.64%,材料间的遗传相似系数(GS)范围为0.64~0.95,平均GS值为0.76。根据研究结果进行聚类分析和主成分分析,可将供试材料分成五大类,分类结果与材料的地理分布大致相符,呈一定的地域性分布规律。由此可见,丰富的地理生态条件造就了狗牙根资源丰富的遗传多样性和明显的地域性分布规律。

Abstract: Amplified fragment length polymorphism(AFLP)molecular markers were applied to detect the genetic variation of 44 agrestal accessions of Cynodon dactylon collected from Africa, Sichuan, Chongqing, Yunnan, Guizhou, and Tibet in China. A total of 462 bands were amplified by ten AFLP primer pairs from C. dactylon genetic DNA, of which 452 (97.64%) bands were found to be polymorphic. The AFLP-based genetic similarity values among 44 C. dactylon accessions ranged from 0.64 to 0.95, and the average Nei’s coefficient was 0.76. Analysis of cluster and principal component analysis showed that all the accessions could be distinguished by AFLP markers and divided into 5 groups. Accessions from the same area were almost all classified into the same group associated with their geographical distributions. Therefore, complex geographical ecological environments are important factors for genetic diversity and geographical distribution of C. dactylon.

中图分类号:

齐晓芳,张新全,凌瑶,陈仕勇,刘伟. 野生狗牙根种质资源的AFLP遗传多样性分析[J]. 草业学报, 2010, 19(3): 155-161.

QI Xiao-fang, ZHANG Xin-quan, LING Yao, CHEN Shi-yong, LIU Wei. Genetic diversity of wild Cynodon dactylon germplasm detected by AFLP markers[J]. Acta Prataculturae Sinica, 2010, 19(3): 155-161.

参考文献

[1] Taliaferro C M. Diversity and vulnerability of bermudagrass courfgrass species[J]. Crop Abstracts, 1995, 35: 327-332.
[2] 张小艾, 张新全, 杨春华, 等. 狗牙根种质资源遗传多样性的研究概况[J]. 草原与草坪, 2003, (3): 17-21.
[3] 谢贤健, 兰代萍, 白景文. 三种野生岩生草本植物的抗旱性综合评价[J]. 草业学报, 2009, 18(4): 75-80.
[4] 尹权为, 曾兵, 张新全, 等. 狗牙根种质资源在渝西地区的生态适应性评价[J]. 草业科学, 2009, (5): 174-178.
[5] Marcone C, Ragozzino A, Seemuller E. Detection of bermudagrass white leaf disease in Italy and characterization of the associated phytoplasma by RFLP analysis[J]. Plant Disease, 1997, 81(8): 862-866.
[6] Roodt R, Spies J J, Burger T H. Preliminary DNA fingerprinting of the turfgrass Cynodon dactylon(Poaceae: Chloridoideae)[J]. Bothealia, 2002, 32: 117-122.
[7] Caetano-Anolles G, Lloyd M, Callahan, et al. The origin of bermudagrass (Cynodon) off-Types inferred by DNA amplification fingerprinting[J]. Crop Science, 1997, 37: 81-87.
[8] Caetano-Anolles G. Genetic instability of bermudagrass (Cynodon) cultivars ‘Tifgreen’ and ‘Tifdrawf’ detected by DAF and ASAP analysis of accession and off-types[J]. Euphytica, 1998, 101: 165-173.
[9] Karaca M, Saha S, Zipf A, et al. Genetic diversity among forage bermudagrass (Cynodon spp.): Evidence from chloroplast and nuclear DNA fingerprinting[J]. Crop Science, 2002, 42: 2118-2127.
[10] Wu Y Q, Taliaferro C M, Bai G H, et al. AFLP analysis of Cynodon dactylon (L.) Pers. var. dactylon genetic variation[J]. Genome, 2004, 47: 689-696.
[11] 梁慧敏. 狗牙根种质资源遗传标记和耐盐性生物技术辅助育种[D]. 北京: 北京林业大学, 2003.
[12] 刘伟. 西南区野生狗牙根种质资源遗传多样性与坪用价值研究[D]. 雅安: 四川农业大学, 2006.
[13] 刘伟, 张新全, 李芳, 等. 西南区野生狗牙根遗传多样性的ISSR标记与地理来源分析[J]. 草业学报, 2007, 16(3): 55-61.
[14] 易杨杰, 张新全, 黄琳凯, 等. 野生狗牙根种质遗传多样性的SRAP研究[J]. 遗传, 2008, 30(1): 94-100.
[15] Zabeau M, Vos P. Selective restriction fragment amplification: A general method for DNA fingerprinting[P]. European Patent Application No. 92402629, Publication No. 0-534-858-A, 1993.
[16] Vos P, Hogers V P, Bleeker R, et al. AFLP: A new technique for DNA fingerprinting[J]. Nucleic Acids Research, 1995, 23: 4407-4414.
[17] 郭雄明, 薛霞, 陈华. AFLP分子标记技术的研究进展[J]. 中国比较医学杂志, 2006, 16(6): 369-372.
[18] 田松杰, 石云素, 宋燕春, 等. 利用AFLP 技术研究玉米及其野生近缘种的遗传关系[J]. 作物学报, 2004, 30(4): 354-359.
[19] 刘欢, 慕平, 赵桂琴. 基于AFLP的燕麦遗传多样性研究[J]. 草业学报, 2008, 17(6): 121-127.
[20] Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases[J]. Proceedings of the National Academy of Science, 1979, 76: 5269-5273.
[21] 徐爱遐, 马朝芝, 肖恩时, 等. 我国西部地区荠菜的遗传多样性研究[J]. 作物学报, 2008, 34(5): 754-763.
[22] 闫龙, 关建平, 宗绪晓. 木豆种质资源AFLP标记遗传多样性分析[J]. 作物学报, 2007, 33(5): 790-798.
[23] Aitken K S, Li J C, Jackson P, et al. AFLP analysis of genetic diversity within Saccharum officinarum and comparison with sugarcane cultivars[J]. Australian Journal of Agricultural Research, 2006, 57: 1167-1184.
[24] Selvi A, Nair N V, Noyer J L, et al. AFLP analysis of the phenetic organization and genetic diversity in the sugarcane complex, Saccharum and Erianthus[J]. Genetic Resources and Crop Evolution, 2006, 53: 831-842.
[25] 王友保, 蒋田华, 安雷, 等. 两种来源狗牙根的生长对铜污染土壤酶活性的影响[J]. 草业学报, 2008, 17(6): 40-46.
[26] Wilson B L, Kitzmiller J, Rolle W, et al. Isozyme variation and its environmental correlates in Elymus glaucus from the California Floristic Province[J]. Canadian Journal of Botany, 2001, 79: 139-153.