半干旱地区禾-豆混播草地生物固氮作用研究

草业学报 ›› 2010, Vol. 19 ›› Issue (6): 276-280.

半干旱地区禾-豆混播草地生物固氮作用研究

王平¹,周道玮^2*,姜世成³

1.东北师范大学城市与环境科学学院,吉林长春 130024;
2.中国科学院东北地理与农业生态研究所,
吉林长春 130012;
3.东北师范大学草地科学研究所,吉林长春 130024

收稿日期:2008-09-17 出版日期:2010-06-25 发布日期:2010-12-20
作者简介:王平(1972-),女,山东平原人,博士。E-mail:wangp744@nenu.edu.cn
基金资助:
国家重点基础研究发展规划项目(G2000018602)和东北师范大学自然科学青年基金(20050401)资助。

Research on biological nitrogen fixation of grass-legume mixtures in a semiarid area of China

WANG Ping1, ZHOU Daowei2，JIANG Shicheng3

1.College of Urban and Environmental Science, Northeast Normal University, Changchun 130024, China;
2.Northeast Institute of Geography and Agroecology, Chinese Academy of Science, Changchun 130012, China;
3.Institute of Grassland Science, Northeast Normal University, Changchun 130024, China

Received:2008-09-17 Online:2010-06-25 Published:2010-12-20

摘要/Abstract

摘要： 本研究以羊草-沙打旺、羊草-兴安胡枝子、羊草-花苜蓿和羊草-紫花苜蓿混播草地为对象,比较4种豆科牧草在松嫩平原半干旱地区的生物固氮能力、氮素转移能力以及生物固氮量对草地氮产量的贡献。结果表明,羊草-沙打旺、羊草-紫花苜蓿混播草地氮产量显著高于单播羊草草地,草地粗蛋白含量的增加显著改善了草地质量。沙打旺、兴安胡枝子、花苜蓿和紫花苜蓿生物固氮率为35%~40%,大气氮是豆科牧草生长不可缺少的氮素来源。豆科牧草向羊草转移氮素2~12kgN/(hm²·a),占羊草氮产量的5%~24%。豆科植物的固氮能力增加了草地氮素资源,沙打旺、兴安胡枝子、花苜蓿和紫花苜蓿生物固氮量分别占其混播草地总氮产量的20.30%,17.78%,11.29%和31.77%。

Abstract: Biological nitrogen fixation (BNF), nitrogen transfer and the contribution of nitrogen derived from air to nitrogen yield were investigated in a semiarid area of China using four grasslegume mixtures: Leymus chinensis-Astragalus adsurgens, L. chinensis-Lespedeza daurica, L. chinensis-Medicago ruthenica, and L. chinensis-M. sativa cv. Aohan. The nitrogen yield of the L. chinensis-Astragalus adsurgens and of the L. chinensis-M. sativa cv. Aohan were significantly greater than that of L. chinensis monoculture and they had an improved grassland quality. The BNF of the four legumes was 35%-40%, suggesting that it was an important nitrogen resource for the growth of legumes. The amount of nitrogen transferred from legume to L. chinensis was 2-12 kg N/(ha·year), about 5%-24% of the nitrogen yield of L. chinensis. Legume BNF promoted the nitrogen resources of grassland ecosystems, contributing 20.30%, 17.78%, 11.29% and 31.77% to total nitrogen yield for A. adsurgens, L. daurica, M. ruthenica, and M. sativa cv. Aohan, respectively.

中图分类号:

Q945.13
S314

王平,周道玮,姜世成. 半干旱地区禾-豆混播草地生物固氮作用研究[J]. 草业学报, 2010, 19(6): 276-280.

WANG Ping1, ZHOU Daowei2，JIANG Shicheng3. Research on biological nitrogen fixation of grass-legume mixtures in a semiarid area of China[J]. Acta Prataculturae Sinica, 2010, 19(6): 276-280.

参考文献

[1] Cleveland C C, Townsend A R, Schimel D S, et al. Global patterns of terrestrial biological nitrogen (N₂) fixation in natural ecosystems[J]. Global Biogeochemical Cycles, 1999, 13: 623-645.
[2] Jenkinson D S. Nitrogen in a global perspective, with focus on temperate areas-state of the art and global perspectives[J]. Plant and Soil, 2001, 228: 3-15.
[3] 姜薇, 关秀清, 于井朝. 生物固氮在集约化草地畜牧业中的作用[J]. 草业学报, 2003, 12(6): 42-46.
[4] Whiter J, Hodgson J. New Zealand Pasture and Crop Science[M]. New York: Oxfored University Press, 1999: 1-10.
[5] Ledgard S F, Steele K W. Biological nitrogen fixation in mixed legume/grass pastures[J]. Plant and Soil, 1992, 141: 137-153.
[6] Sinclair T R, Rachid S, Legume nitrogen fixation and drought[J]. Nature, 1995, 378 (23): 344.
[7] 王平, 王天慧, 周雯, 等. 禾-豆混播草地中土壤水分与种间竞争关系研究进展[J]. 应用生态学报, 2007, 18(3): 653-658.
[8] 慈恩, 高明. 环境因子对豆科共生固氮影响的研究进展[J]. 西北植物学报, 2005, 25(6): 1269-1274.
[9] 樊江文, 高永革. 混播草地中豆科牧草的固氮作用[J]. 中国草地, 1994, (6): 64-69, 73.
[10] 王卫卫, 胡正海. 几种生态因素对西北干旱地区豆科植物结瘤固氮的影响[J]. 西北植物学报, 2003, 23(7): 1163-1168.
[11] Mooso G D, Wedin W F. Yield dynamics of canopy components in alfalfa-grass mixtures[J]. Agronomy Journal, 1990, 82: 696-701.
[12] 王平, 周道玮, 姜世成. 中国半干旱地区禾豆混播草地草产量和氮产量[J]. 草业学报, 2006, 15(增刊): 166-168.
[13] Thomas R J. Role of legumes in proving N for sustainable tropical pasture systems[J]. Plant and Soil, 1995, 174: 103-118.
[14] Townsend C E. Breeding, physiology, culture, and utilization of cicer milkvetch (Astragalus cicer L.)[J]. Advances in Agronomy, 1993, 49: 253-308.
[15] 王元素, 蒋文兰, 洪绂曾, 等. 白三叶与不同禾草混播群落17年稳定性比较研究[J]. 草业学报, 2006, 15(3): 55-62.
[16] Wahua T A T, Miller D A. Effects of intercropping on soybean nitrogen fixation and plant composition on associated sorghum and soybeans[J]. Agronomy Journal, 1978, 70: 292-295.
[17] Ramos M L G, Gordon A J, Minchin F R, et al. Effect of water stress on nodule physiology and biochemistry of a drought tolerant cultivar of common bean (Phaseolus vulgaris L.)[J]. Annals of Botany, 1999, 83: 57-63.
[18] 梁建生, 张建华, 曹显祖. 呼吸活性的下降可解释水分亏缺对银合欢根瘤固氮酶活性的抑制[J]. 植物生理学报, 1998, 24(3): 285-292.
[19] Walley F L, Tomm G O, Matus A, et al. Allocation and cycling of nitrogen in an alfalfa-bromegrass sward[J]. Agronomy Journal, 1996, 88: 834-843.
[20] Trannin W S. Interspecies competition and N transfer in a tropical grass-legume mixture[J]. Biology and Fertility of Soils, 2000, 32(6): 441-448.
[21] Zhu Y G, Laidlaw A S, Christie P, et al. The specificity of arbuscular mycorrhizal fungi in perennial ryegrass-white clover pasture[J]. Agriculture, Ecosystems and Environment, 2000, 77(3): 211-218.
[22] 李小坤, 鲁剑巍, 陈防. 牧草施肥研究进展[J]. 草业学报, 2008, 17(2): 136-142