Abstract: Salt stress affects plant growth and development and reduces productivity of crops. Glycine soja is an excellent material for isolation of abiotic stress related genes because of its tolerance to several kinds of stresses. An EST database of a salt tolerant variety of G. soja was constructed in previous work, and a gene expression profile was obtained by gene chip assay. Expression of all G. soja ESTs were analyzed, and one 3′-EST induced by cold, high salt and drought stress was chosen. This EST represents a plasma membrane intrinsic protein (PIP) gene, which belongs to the major intrinsic protein (MIP) family. A complete CDS of this gene was obtained with silico cloning and was developed with 5′-RACE. The translation product of this gene has 90% similarity with a PIP protein of Mimosa pudica and was named GsPIP. The translation product had the characteristic domain of MIP but has no signal peptide: This is consistent with PIPs of other plants. We isolated the GsPIP gene on our own and we have all intellectual property rights. It could become a new gene source for genetic engineering of stress tolerance. Further functional analysis would assist research on the mechanisms of osmotic stress tolerance.