菊苣叶提取物对粘虫的生物活性研究

草业学报 ›› 2011, Vol. 20 ›› Issue (2): 60-66.

菊苣叶提取物对粘虫的生物活性研究

孙娈姿,王佺珍,呼天明^*,杜金鸿

西北农林科技大学动物科技学院,陕西杨凌 712100

收稿日期:2010-04-01 出版日期:2011-02-25 发布日期:2011-04-20
通讯作者: E-mail: hutianming@126.com
作者简介:孙娈姿 (1984-),女,辽宁大连人,在读硕士。E-mail:sunluanzi@163.com
基金资助:
科技部国际合作项目(普那菊苣天然产物提纯鉴定的合作研究,2008DFA31650)和陕西省国际合作项目(普那菊苣天然产物提纯鉴定合作研究,2008KW-29)资助。

Bioactivity of Cichorium intybus leaf extracts against Mythimna separata

SUN Luan-zi, WANG Quan-zhen, HU Tian-ming, DU Jin-hong

College of Animal Science & Technology, Northwest A&F University, Yangling 712100, China

Received:2010-04-01 Online:2011-02-25 Published:2011-04-20

摘要/Abstract

摘要： 为研究菊苣叶提取物的杀虫活性,采用冷浸法和超声波混合提取法得到菊苣叶提取物,对3及4龄粘虫分别用饲喂称重法和叶碟添加法进行毒力测定。结果表明,各有机溶剂提取物处理的幼虫体重均显著低于对照组(P＜0.05);乙醇和乙酸乙酯提取物处理组的幼虫期比对照组延长了4.3 d;各有机溶剂提取物处理组中,乙酸乙酯提取物死亡率最高,为57.78%,其化蛹率最低,仅为46.67%;各处理组蛹重均显著大于对照组(P＜0.05) ;乙醇和乙酸乙酯提取物处理组粘虫的拒食率高达80%以上。

Abstract: Insecticidal activity of extracts from leaves of Cichorium intybus, were assessed by cold-soaked extraction and by microwave-assisted extraction. The insecticidal activities of the C. intybus leaf extracts against the larvae of Mythimna separata were tested by a feeding, weighing and leaf disk method. The body weights of larvae fed by the extracts with organic solvents were significantly lower than those of the controls in the first seven days, and the development period of larvae treated with ethanol or ethyl acetate extracts were 4.3 days longer than that of the controls. The corrected mortality of M. separata treated by the ethyl acetate extracts was the highest (57.78%), and the corresponding pupating rate was the lowest (46.67%). Moreover, the pupal weight in the different treatments was greater than that of the controls. In anti-feeding experiments, the anti-feeding rates of ethanol extracts and ethyl acetate extracts against M. separata were more than 80%.

中图分类号:

S482.3

孙娈姿,王佺珍,呼天明,杜金鸿. 菊苣叶提取物对粘虫的生物活性研究[J]. 草业学报, 2011, 20(2): 60-66.

SUN Luan-zi, WANG Quan-zhen, HU Tian-ming, DU Jin-hong. Bioactivity of Cichorium intybus leaf extracts against Mythimna separata[J]. Acta Prataculturae Sinica, 2011, 20(2): 60-66.

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