镉胁迫下多年生黑麦草的光合生理响应

草业学报 ›› 2012, Vol. 21 ›› Issue (3): 191-197.

镉胁迫下多年生黑麦草的光合生理响应

刘俊祥^1,2,孙振元^1,2*,勾萍³,钱永强^1,2,巨关升^1,2,韩蕾^1,2

1.中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京 100091;
2.林木遗传育种国家重点实验室中国林业科学研究院林业研究所,北京 100091;
3.沈阳农业大学林学院,辽宁沈阳 110161

出版日期:2012-06-20 发布日期:2012-06-20
通讯作者: E-mail:sunzy@263.net
作者简介:刘俊祥(1981-),男,辽宁本溪人,在读博士。
基金资助:
国家“863”项目(2011AA100209)资助。

Response of photosynthetic physiology of perennial ryegrass (Lolium perenne) to Cd²⁺ stress

LIU Jun-xiang^1,2, SUN Zhen-yuan^1,2, GOU Ping³, QIAN Yong-qiang^1,2, JU Guan-sheng^1,2, HAN Lei^1,2

1.Research Institute of Forestry, Chinese Academy of Forestry /Key Laboratory of Forest Cultivation, State Forestry Administration, Beijing 100091,China;
2.State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;
3.College of Forestry, Shenyang Agricultural University, Shenyang 110866, China)

Online:2012-06-20 Published:2012-06-20

摘要/Abstract

摘要： 以多年生黑麦草为植物材料, 对其进行0(CK),5,10,20 mg/L的Cd²⁺处理6 d,结果表明,Cd²⁺处理显著降低了地上部、地下部的生物量;随着处理浓度的升高,Cd在地上部、地下部的含量显著升高,地上部Cd的分布比例显著升高,地下部Cd的分布比例显著降低,多年生黑麦草对Cd具有超量吸收和转运的能力。在处理的0,0.25,1,3,6 d,测定5 mg/L Cd²⁺处理的多年生黑麦草叶片的气体交换参数、光合色素含量和叶绿素荧光参数,结果表明, Cd²⁺处理未导致光合色素含量的变化,气体交换参数和叶绿素荧光参数均受到显著的影响。处理1 d内,净光合速率(Pn)、气孔导度(Gs)和胞间二氧化碳浓度(Ci)具有相同的变化趋势,Pn的下降是由气孔因素导致的。Pn在处理3和6 d分别较未处理显著下降了24.4%和23.4%,非气孔因素对Pn的降低起到了主要作用。蒸腾速率(Tr)在处理1 d较未处理显著上升了74.1%,这有助于Cd向地上部转运。潜在光化学效率(F_v/F₀)在处理的0.25,1,3 d时分别较未处理显著上升了14.4%,21.0%和14.4%。最大光化学效率(F_v/F_m)在处理0.25,1,3 d分别较未处理显著上升了3.1%,3.2%和3.1%。实际光化学效率(ΦPSⅡ)、相对电子传递效率(rETR)和光化学淬灭系数(qP)在处理6 d较未处理显著下降了25.3%,25.3%和21.1%,叶片PSⅡ的光化学反应能力显著降低。非光化学淬灭系数(qN)在处理0.25,1 d分别较未处理显著下降了15.6%,41.5%,随着处理时间的延长,qN值显著升高,起到了光保护的作用。非调节性能量耗散在处理0.25,1 d分别较未处理显著上升了30.8%,37.8%,处理3和6 d下降至与未处理无显著差异。调节性能量耗散在处理1 d比未处理显著下降了36.9%,在6 d时显著上升了17.0%,减轻了光合机构的伤害程度。

Abstract: Effects of Cd²⁺ on biomass of Lolium perenne were studied, and Cd accumulative characteristics were also elaborated. L. perenne were treated with Cd²⁺(0, 5, 10, 20 mg/L) for 6 days. The biomass of aboveground and underground decreased significantly . With the Cd²⁺ concentration increasing, the Cd content in L. perenne increased significantly, and the distribution proportion of Cd in underground decreased significantly, contrary in the aboveground. L. perenne had the excessive ability in absorbing and transporting Cd. In order to explore the photosynthetic physiology response of L. perenne to Cd²⁺ stress, explaining the biomass change affected by Cd²⁺, L. perenne were treated with Cd²⁺(5 mg/L) for 0, 0.25, 1, 3, 6 days respectively. The photosynthetic pigments contents did not change significantly, whereas the gas exchange parameters and the chlorophyll fluorescence parameters were affected significantly. Within 1 d, the change of Pn, Gs and Ci were consistent, the reduce of net photosynthesis rate was caused by stomatal limitation factors. When the plants were treated 3 and 6 d, Pn significantly decased by 24.4% and 23.4% compared with untreated, the recuce was caused by non-stomatal limitation factors. Tr significantly increased by 74.1% when treated 1 d, this promoted Cd transporting to aboveground. Within 3 d, F_v/F₀ significantly increased by 14.4%, 21.0%, 14.4% compared with untreated, and F_v/F_m significantly increased by 3.1%, 3.2%, 3.1% respectively. ΦPSⅡ, rETR and qP significantly decreasd by 25.3%,25.3%,21.1% when treated 6 d, the PSⅡ light reaction ability reduced. The qN significantly decreasd by 15.6% and 41.5% when treated 0.25 and 1 d, then significantly increasd, the photoprotective capacity was increased. Compared with untreated, Y(NO) significantly increased by 30.8% and 37.8% when treated 0.25 and 1 d, and decreased significantly when 3 and 6 d. Y(NPQ) significantly decreased by 36.9% when treated 1 d, whereas significantly incerased by 17.0% when treated 6 d, the extent of photosynthetic apparatus damage was reduced.

中图分类号:

刘俊祥,孙振元,勾萍,钱永强,巨关升,韩蕾. 镉胁迫下多年生黑麦草的光合生理响应[J]. 草业学报, 2012, 21(3): 191-197.

LIU Jun-xiang, SUN Zhen-yuan, GOU Ping, QIAN Yong-qiang, JU Guan-sheng, HAN Lei. Response of photosynthetic physiology of perennial ryegrass (Lolium perenne) to Cd²⁺ stress[J]. Acta Prataculturae Sinica, 2012, 21(3): 191-197.

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