苜蓿乙烯应答因子基因的表达特性和生物信息学分析

草业学报 ›› 2012, Vol. 21 ›› Issue (6): 166-174.

苜蓿乙烯应答因子基因的表达特性和生物信息学分析

陈婷婷^1,2,杨青川^1*,张新全²,康俊梅¹,丁旺¹,张铁军¹

1.中国农业科学院北京畜牧兽医研究所,北京 100193;
2.四川农业大学草业科学系,四川雅安 625014

收稿日期:2011-11-15 出版日期:2012-06-25 发布日期:2012-12-20
通讯作者: E-mail:qchyang66@yahoo.com.cn
作者简介:陈婷婷(1987-),女,山东曹县人,在读硕士。E-mail:chentingting8701@163.com
基金资助:
国家现代牧草产业技术体系(CARS-35)和国家自然科学基金(30871819)资助。

Bioinformatics and expression analyses of ethylene response factor genes in Medicago

CHEN Ting-ting^1,2,YANG Qing-chuan¹, ZHANG Xin-quan², KANG Jun-mei¹, DING Wang¹, ZHANG Tie-jun¹

1.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2.Department of Grassland Science, Sichuan Agricultural University, Ya’an 625014, China

Received:2011-11-15 Online:2012-06-25 Published:2012-12-20

摘要/Abstract

摘要： 乙烯应答因子(ethylene response factor,ERF)是植物特有的一类转录因子,参与植物生长发育和胁迫应答等多个生理生化过程。本研究以拟南芥AP2结构域为探针搜索美国国立生物技术信息中心(NCBI)的苜蓿属蛋白质数据库,运用生物信息学软件分析苜蓿乙烯应答因子的系统进化、疏水轮廓、二级结构、信号肽以及亚细胞定位情况。运用半定量RT-PCR技术研究5个紫花苜蓿乙烯应答因子基因(MsERF1, MsERF2,MsERF6, MsERF7, MsERF9)表达的组织差异性以及多种处理条件下的表达特性。结果表明,5个乙烯应答因子基因在叶中的表达量都明显高于其他组织,不同基因有不同的组织表达特性;NaCl和PEG6000对MsERF2的表达没有影响,但Al₂(SO₄)₃能诱导其表达,其余基因的表达都受这3种处理的诱导;脱落酸对MsERF2的表达没有影响,能够诱导其他基因的表达;生长素只能诱导MsERF6的表达,对其他基因的表达没有影响;赤霉素、茉莉酸甲酯和乙烯利对5个基因的表达都起促进作用。本研究为进一步研究苜蓿乙烯应答因子的结构和功能奠定了基础,同时为研究不同信号传导途径之间的相互作用提供了依据。

Abstract: Ethylene response factors are plant-special transcription factors involved in plant growth and development. The Arabidopsis AP2 domain was used as a probe to search the NCBI Medicago protein database and to analyze the phyletic evolution, secondary structure, hydrophobicity, signal peptide, subcellular localization of Medicago ethylene response factors using bioinformatics software. Primers were designed based on the sequences obtained to analyze the expression patterns of MsERF genes (MsERF1, MsERF2, MsERF6, MsERF7, MsERF9). The MsERF genes had high expression levels in leaves and showed significant differences of expression in different tissues. The expression of MsERF2 was not induced by NaCl and PEG-6000, but was promoted by Al₂(SO₄)₃. The expression of other genes was induced by these three treatments, but the expression patterns were different. ABA reduced the expression of these genes except for MsERF2. IAA had no effect on the expression of these genes except MsERF6. GA₃, MeJA, and Eth reduced the expression of all five genes. This research established a solid foundation to further study the function of these genes and provided evidence to study the cross-talk in different signal pathways.

中图分类号:

陈婷婷,杨青川,张新全,康俊梅,丁旺,张铁军. 苜蓿乙烯应答因子基因的表达特性和生物信息学分析[J]. 草业学报, 2012, 21(6): 166-174.

CHEN Ting-ting,YANG Qing-chuan, ZHANG Xin-quan, KANG Jun-mei, DING Wang, ZHANG Tie-jun. Bioinformatics and expression analyses of ethylene response factor genes in Medicago[J]. Acta Prataculturae Sinica, 2012, 21(6): 166-174.

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