海滨雀稗体细胞突变体SP2008-3的特异性分析

草业学报 ›› 2012, Vol. 21 ›› Issue (6): 207-212.

海滨雀稗体细胞突变体SP2008-3的特异性分析

常盼盼^1,2,钟小仙^1*,刘智微¹

1.江苏省农业科学院畜牧研究所,江苏南京 210014;
2.南京农业大学动物科技学院草业科学系,江苏南京 210095

收稿日期:2011-12-05 出版日期:2012-06-25 发布日期:2012-12-20
通讯作者: E-mail: zhpansy@yahoo.com.cn
作者简介:常盼盼(1987-),女,黑龙江密山人,在读硕士。E-mail:cpp2011236@163.com
基金资助:
宁波市科技计划项目 (2008C10033)和慈溪市农业社会发展科技计划(CR200723)资助。

Comparison and analysis of the characteristics of mutant SP2008-3 and adalayd in seashore Paspalum

CHANG Pan-pan^1,2, ZHONG Xiao-xian¹, LIU Zhi-wei¹

1.Institute of Animal Science, Jiangsu Academy of Agricultural Science, Nanjing 210014, China;
2.Department of Grass Science, Animal Science and Technology College, Nanjing Agricultural University, Nanjing 210095, China

Received:2011-12-05 Online:2012-06-25 Published:2012-12-20

摘要/Abstract

摘要： 以海滨雀稗体细胞突变体SP2008-3和Adalayd(对照)为材料,对其植物学特征和叶片表皮气孔特性进行了比较,结合染色体鉴定和RAPD技术,探索了海滨雀稗体细胞突变体SP2008-3的特异性。结果表明,与对照Adalayd相比,SP2008-3匍匐茎节间长、茎直径分别显著减小34.77%和11.76%,叶片长度、宽度分别显著增加22.55%和11.11%,而直立茎节间长、茎直径无显著差异;叶片上表皮气孔器密度显著增加20.58%,气孔器长度和宽度分别显著减小9.64%和6.31%;叶片下表皮气孔器密度显著增加40.62%,气孔器长度和宽度分别显著减小6.73%和5.33%。SP2008-3体细胞染色体数目为2n=20,与对照Adalayd染色体数目相同。RAPD结果显示,采用随机引物S369扩增,SP2008-3缺失1条约1 100 bp的条带,表明其在DNA水平上与Adalayd存在差异。

Abstract: A comparison of botanical properties, of stomatal characteristics of the upper and lower leaf epidermis, and of chromosome identification and RAPD analysis between mutant SP2008-3 and Adalayd in seashore Paspalum was carried out. Stolon internode length and stem diameter were reduced by 34.77% and 11.76% in SP2008-3 compared with Adalayd and leaf length and width were increased by 22.55% and 11.11% respectively. There were no significant differences in erect stem internode length and diameter between SP2008-3 and Adalayd. In the upper leaf epidermis of SP2008-3, stomatal density was 20.58% greater, and stomatal length and width were 9.64% and 6.31% less respectivley than in Adalayd. Stomatal density in the lower leaf epidermis of SP2008-3, was 40.62% greater, and stomatal length and width were 6.73% and 5.33% less than in Adalayd. The somatic chromosome number was 2n=20 in both SP2008-3 and Adalayd. RAPD analysis showed a difference in the DNA: a 1 100 bp band was missing in SP2008-3 when the DNA was amplified by primer S369.

中图分类号:

Q944.3

常盼盼,钟小仙,刘智微. 海滨雀稗体细胞突变体SP2008-3的特异性分析[J]. 草业学报, 2012, 21(6): 207-212.

CHANG Pan-pan, ZHONG Xiao-xian, LIU Zhi-wei. Comparison and analysis of the characteristics of mutant SP2008-3 and adalayd in seashore Paspalum[J]. Acta Prataculturae Sinica, 2012, 21(6): 207-212.

参考文献

[1] 解新明, 卢小良. 海雀稗种质资源的优良特性及其利用价值[J]. 华南农业大学学报, 2004, 25: 64-67.
[2] Duncan R R, Carrow R N. Seashore Paspalum-the Environmental Turfgrass[M]. Hoboken: John Wiley＆Sons, Inc., 1999.
[3] Peacock C H, Dudeck A E. Physiological and growth responses of seashore paspalum to salinity[J]. HortScience, 1985, 20(1): 111-112.
[4] Duncan R R. Seashore paspalum may be grass for the year 2000[J]. Southern Turf Management, 1994, 5: 31-32.
[5] Huang B, Duncan R R, Carrow R N. Drought-resistance mechanisms of seven warn-season turfgrass under surface soil drying:I.Shoot response[J]. Crop Science, 1997, 37(6): 1858-1863.
[6] 贺小霞, 刘一明, 王兆龙. 海滨雀稗栽培品种的形态特征与AFLP分子标记分析[J]. 草地学报, 2011, 19(1): 164-170.
[7] 郑轶琦, 臧国长, 郭海林, 等. 假俭草杂交后代生殖性状遗传及相关性分析[J]. 草业学报, 2011, 20(2): 283-289.
[8] 邹轶, 顾洪如, 钟小仙, 等. 海盐胁迫对海滨雀稗生长及植株体内阳离子含量的影响[J]. 草业科学, 2009, 26(4): 117-120.
[9] 景艳霞, 袁庆华. NaCl胁迫对苜蓿幼苗生长及不同器官中盐离子分布的影响[J]. 草业学报, 2011, 20(2): 134-139.
[10] 邹轶, 顾洪如, 钟小仙, 等. 海盐胁迫对海滨雀稗生长及植株体内阳离子含量的影响[J]. 草业科学, 2009, 24(6): 117-120.
[11] Espinoza F, Quarin C L. Cytoembryology of Paspalum chaseanum and sexual diploid biotypes of two apomictic Paspalum species[J]. Australian Journal of Botany, 1997, 45: 871-877.
[12] Carpenter J A. Production and use of seed in seashore paspalum[J]. Australian Institute of Agricultural Science and Technology, 1958, 24: 252-256.
[13] Fricker C, Wipff J K, Duncan R R. Hybrid seashore paspalum available from seed called“Sea Spray”[P]. United States Patent: 7262341B1, 2007-08-28.
[14] 钟小仙, 吴娟子, 刘智微, 等. 自交不结实海雀稗秋水仙素诱导体细胞突变体的筛选方法[P]. 中国专利: 201110074147.1, 2011-03-28.
[15] 马金星, 张吉宇, 单丽燕, 等. 中国草品种审定登记工作进展[J]. 草业学报, 2011, 20(1): 206-213.
[16] 梁流芳, 佘建明, 吴瑛瑛, 等. 海雀稗幼穗离体培养植株再生[J]. 草地学报, 2008, 16(6): 590-593.
[17] 孙同兴, 江幸山. 简便有效的叶表皮离析方法——过氧化氢-醋酸法[J]. 广西植物, 2009, 29(1): 44-47.
[18] 傅志强, 黄璜, 何保良, 等. 水稻叶片气孔特性及其相关性[J]. 湖南农业大学学报: 自然科学版, 2007, 33(6): 646-650.
[19] 虞道耿, 刘国道, 白昌军, 等.热研11号黑籽雀稗染色体核型分析[J].草业科学, 2010, 27(1): 109-113.
[20] 李懋学, 张赞平. 作物染色体及其研究技术[M]. 北京: 中国农业出版社, 1996: 1-60.
[21] 张建成, 江玉萍, 王传堂, 等. 花生品种鉴定技术研究进展[J]. 花生学报, 2006, 35(2): 24-28.
[22] 刘建秀, 贺善安, 刘永东, 等. 华东地区狗牙根形态分类及其坪用价值[J]. 植物资源与环境, 1996, 5(3): 18-22.
[23] 金洪, 韩烈保, 张永明. 中国结缕草居群形态变异分析[J]. 中国草地, 2004, 26(2): 50-56.
[24] 覃秀菊, 李凤英, 何建栋, 等. 广西茶树新品种品系叶片解剖结构特征与特性关系的研究[J]. 中国农学通报, 2009, 25(10): 36-39.
[25] 李宗艳, 肖娟, 蒙进芳, 等. 丽江牡丹和中原牡丹叶片结构微形态比较[J]. 浙江农林大学学报, 2011, 28(1): 115-120.
[26] Casson S A, Hetherington A M. Evironmental regulation of stomatal development[J]. Current Opinion in Plant Biology, 2010, 13(1): 90-95.
[27] Aschan G, Pfanz H, Vodnik D, et al. Photosynthetic performance of vegetative and reproductive structures of green hellebore (Helleborus viridis L. agg.)[J]. Photosynthetica, 2005, 43(1): 55-64.
[28] 游明安, 盖钧镒, 马育华. 田间条件下大豆气孔特性的初步研究[J]. 大豆科学, 1992, 11(2): 152-158.
[29] 郑永强, 徐坤. 秋水仙素在植物体细胞染色体加倍中的应用研究进展[J]. 中国农学通报, 2003, 19(5): 89-91.
[30] 徐明, 路铁刚. 植物诱变技术的研究进展[J]. 生物技术进展, 2011, 1(2): 90-97.
[31] 甘玲, 汤浩茹, 董晓莉. 梨种类和品种鉴定研究进展[J]. 中国农学通报, 2006, 22(5): 302-307.
[32] 陈智勇, 胡忠红, 蒋建雄, 等. 早熟禾族3种基因型草坪草的染色体核型分析[J]. 草业学报, 2010, 19(6): 281-285.
[33] 李志勇, 孙启忠, 李鸿雁, 等. 分子标记技术在牧草种质资源研究中的应用[J]. 草原与草坪, 2010, 30(5): 91-96.
[34] 解新明, 卢小良, 孙雄松, 等. 海雀稗种质资源分子标记的遗传多样性研究[J]. 华南农业大学学报, 2004, 25: 10-15.
[35] Martelotto L G, Ortiz J P A, Stein J, et al. Genome rearrangements derived from autopolyploidization in Paspalum sp.[J]. Plant Science, 2007, 172: 970-977.