20个黑麦草品系的SRAP遗传多样性分析

草业学报 ›› 2013, Vol. 22 ›› Issue (2): 158-164.

20个黑麦草品系的SRAP遗传多样性分析

李杰勤¹, 王丽华¹,詹秋文^1*,朱奎¹,刘井良²,李万祥²

1.安徽科技学院植物科学学院,安徽凤阳 233100;
2.安徽金沱湖蟹业有限责任公司,安徽五河 233300

收稿日期:2012-11-02 出版日期:2013-02-25 发布日期:2013-04-20
作者简介:李杰勤(1980-),男,四川宜宾人,讲师,硕士。E-mail:wlhljq@163.com
基金资助:
安徽高等学校省级自然科学研究项目(No:KJ2011Z075),安徽科技学院青年基金(ZRC2011280),国家自然科学基金(No: 31071470),安徽省“115”产业创新团队(皖人才办[2009]2号)和科技部“十二五”农村领域国家科技计划课题(No:2011BAD17B03)资助。

Genetic diversity of 20 ryegrass accessions by SRAP markers

LI Jie-qin¹, WANG Li-hua¹, ZHAN Qiu-wen¹, ZHU Kui¹, LIU Jing-liang², LI Wan-xiang²

1.College of Plant Science and Technology, Anhui Science and Technology University, Fengyang 233100, China;
2.Anhui Golden Tuo Lake Crab Limited Company, Wuhe 233300, China

Received:2012-11-02 Online:2013-02-25 Published:2013-04-20

摘要/Abstract

摘要： 利用SRAP分子标记技术对20个黑麦草品系的遗传多样性进行了分析。结果发现,53个引物组合共扩增出765条带,其中多态性带共597条,多态性比率为78.95%。平均每对引物扩增出的带数和多态性条带数分别为14.4和11.2条。各黑麦草品系间的遗传相似系数在0.485～0.727,平均为0.627。从UPGMA聚类分析的结果来看,以阈值0.63可将这20个品系分为4类。2个多花黑麦草品系并不聚在一类,而是分散在18个多年生黑麦草品系中。另外,UPGMA聚类结果与这些品系的来源基本一致,这说明SRAP标记适用于黑麦草品系间的遗传多样性研究。

Abstract: Sequence related amplified polymorphism (SRAP) markers were used to analyze the genetic diversity among 20 ryegrass (Lolium) lines. The results showed that 765 fragments were amplified by 53 primers. Polymorphic bands were 597 which accounted for 78.95% in the total amplified fragments. The number of amplified and polymorphic fragments was 14.43 and 11.26 per primer, respectively. The genetic similarity coefficients ranged from 0.485 to 0.727 among these ryegrass lines and the average was 0.627. Using unweighted pair-group method with arithmetic means (UPGMA) cluster analysis on the SRAP markers, these ryegrass lines could be divided into four groups by the threshold of 0.63. Two Lolium multiflorum lines could not be clustered into a group but spread in 18 Lolium perenne lines. In addition, cluster results were consistent with the resources of these ryegrass lines. All results suggested that SRAP markers were suitable for the research of the genetic diversity on ryegrass.

中图分类号:

李杰勤, 王丽华,詹秋文,朱奎,刘井良,李万祥. 20个黑麦草品系的SRAP遗传多样性分析[J]. 草业学报, 2013, 22(2): 158-164.

LI Jie-qin, WANG Li-hua, ZHAN Qiu-wen, ZHU Kui, LIU Jing-liang, LI Wan-xiang. Genetic diversity of 20 ryegrass accessions by SRAP markers[J]. Acta Prataculturae Sinica, 2013, 22(2): 158-164.

参考文献

[1] 杨青川, 王坤. 牧草的生产与利用[M]. 北京: 化学工业出版社, 2002: 111-112.
[2] 刘俊祥, 孙振元, 勾萍, 等. 镉胁迫下多年生黑麦草的光合生理响应[J]. 草业学报, 2012, 21(3): 191-197.
[3] 曾琨, 张新全. 黑麦草属种质资源遗传多样性研究[J]. 安徽农业科学, 2007, 35(11): 3252-3254.
[4] 刘建新, 王鑫, 王瑞娟, 等. 黑麦草对NaHCO₃胁迫的光合生理响应[J]. 草业学报, 2012, 21(3): 184-190.
[5] 全国牧草品种审定委员会. 中国牧草登记品种集[M]. 北京: 中国农业大学出版社, 1999: 42-43.
[6] Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica[J]. Theoretical and Applied Genetics, 2001, 103: 455-461.
[7] 陈罡, 邢兆凯, 潘文利, 等. 辽宁地区主栽杨树品种的SRAP标记遗传多样性分析[J]. 东北林业大学学报, 2010, 38(10): 19-22.
[8] 窦美安, 邱文武, 吴青松, 等. 菠萝遗传多样性的SRAP分析[J]. 果树学报, 2010, 27(6): 930-937.
[9] 房超, 杨志荣, 刘独臣, 等. 应用SRAP分子标记构建茄子遗传图谱初探[J]. 西南农业学报, 2010, 23(5): 18-22.
[10] 沈志军, 马瑞娟, 俞明亮, 等. 油蟠桃组合遗传连锁图谱构建及糖酸性状QTL分析[J]. 园艺学报, 2010, 37(11): 1735-1744.
[11] 周延清. DNA 分子标记技术在植物研究中的应用[M]. 北京: 化学工业出版社, 2005: 13-15.
[12] Rohlf F J. NTSYS-pc: numerical taxonomy and multivariate analysis system version 2.02h[S]. New York: Exeter Software, 1997.
[13] Ferriol M, Pico B, De Cordova P F, et al. Molecular diversity of a germplasm collection of squash (Cucurbita moschata) determined by SRAP and AFLP marker[J]. Crop Science,2004,44: 65-69.
[14] 刘月光, 滕永勇, 潘辰, 等. 应用SRAP标记对莲藕资源的聚类分析[J]. 氨基酸和生物资源, 2006, 28(1): 29-32.
[15] 季杨, 张新全, 马啸, 等. 多花黑麦草品种(系)间杂交及其杂种后代SRAP遗传分析[J]. 草业学报, 2009, 18(4): 260-265.
[16] 杨文轩, 马啸, 张新全, 等. 多花黑麦草品种间杂交F₂代分子标记遗传分析[J]. 草业学报, 2012, 21(4): 125-133.
[17] 董晓宁, 张晓佩, 李文杨. 18个黑麦草品种(系)的RAPD分析[J]. 福建农业学报, 2009, 24(3): 266-269.
[18] 宁婷婷, 张再君, 金诚赞, 等. 用RAPD分析多年生黑麦草品种间遗传多样性[J]. 武汉植物学研究, 2005, 23(1): 27-31.
[19] Roland-ruiz I F, Van Eeuwijk A, Gilliland T J. A comparative study of molecular and morphological methods of describing relationships between perennial ryegrass (Lolium perenne L.) varieties[J]. Theoretical and Applied Genetics, 2001, 103: 1138-1150.