外源NO对镉胁迫下玉米幼苗生长和生理特性的影响

草业学报 ›› 2013, Vol. 22 ›› Issue (2): 178-186.

外源NO对镉胁迫下玉米幼苗生长和生理特性的影响

王芳^1,2,3,常盼盼¹,陈永平¹,彭云玲^1,2,3,方永丰^1,2,3,王汉宁^1,2,3*

1.甘肃农业大学农学院,甘肃兰州 730070;
2.甘肃省干旱生境作物学重点实验室,甘肃兰州 730070;
3.甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州 730070

收稿日期:2012-09-19 出版日期:2013-02-25 发布日期:2013-04-20
通讯作者: E-mail:wanghn@gsau.edu.cn
作者简介:王芳(1980-),女,甘肃民勤人,讲师,在读博士。E-mail:wangfang@gsau.edu.cn
基金资助:
973计划前期研究专项(2012CB722902)和甘肃农业大学盛彤笙科技创新基金(GSAU-STS-1229)资助。

Effect of exogenous nitric oxide on seedling growth and physiological characteristics of maize seedlings under cadmium stress

WANG Fang^1,2,3, CHANG Pan-pan¹, CHEN Yong-ping¹, PENG Yun-ling^1,2,3, FANG Yong-feng^1,2,3, WANG Han-ning^1,2,3

1.College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China;
2.Drought Environment Crop Cultivation of Key Laboratory in Gansu Province, Lanzhou 730070, China;
3.Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou 730070, China

Received:2012-09-19 Online:2013-02-25 Published:2013-04-20

摘要/Abstract

摘要： 采用盆栽试验,研究了外源NO对镉(Cd)胁迫下玉米幼苗膜脂过氧化、渗透调节物质、抗氧化酶活性及叶绿素含量等生理特性的影响。结果表明,与对照比较,100 mg/L的Cd处理降低了玉米幼苗脯氨酸(Pro)和叶绿素含量,减缓了可溶性蛋白和可溶性糖含量的积累,抗氧化酶活性下降,丙二醛(MDA)含量和超氧阴离子(O₂^-·)产生速率增加、质膜透性增大;与100 mg/L Cd处理比较,添加100 μmol/L硝普钠(SNP)处理显著增加了Cd胁迫下玉米幼苗生物量,提高了超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性,增加了幼苗叶绿素和脯氨酸含量、促进了可溶性糖和可溶性蛋白的积累,降低了MDA含量、细胞质膜的透性以及O₂^-·产生的速率。因此,外源NO对Cd胁迫下玉米生长有一定的缓解作用,可以增强玉米幼苗对Cd毒害的抗性。

Abstract: The present study was conducted in pot to investigate the effects of exogenous nitric oxide (NO) on the membrane lipid permeability, osmotic adjustment substances, antioxidant enzyme activities and chlorophyll content of maize seedling under cadmium stress. The results showed that: compared with the CK, under 100 mg/L Cd²⁺ stress, proline and chlorophyll content were decreased, the accumulation of soluble protein and soluble sugar content were slowly accumulated, and the antioxidant enzymes activities were decreased, but MDA content, O₂^-·generation rate and membrane permeability were increased. Compared with 100 mg/L Cd, 100 μmol/L SNP (sodium nitroprusside)significantly increased biomass amoun and improved the activity of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), increased the content of chlorophyll and proline (Pro), and promoted the accumulation of soluble sugar and soluble protein content, but reduced MDA content, the rate of accumulation of O₂^-·and cell membrane permeability. The results indicated that exogenous nitric oxide had a significantly effect on the growth of seedlings under 100 mg/L Cd²⁺ stress, and enhanced the resistance of maize seedling to Cd toxicity.

中图分类号:

王芳,常盼盼,陈永平,彭云玲,方永丰,王汉宁. 外源NO对镉胁迫下玉米幼苗生长和生理特性的影响[J]. 草业学报, 2013, 22(2): 178-186.

WANG Fang, CHANG Pan-pan, CHEN Yong-ping, PENG Yun-ling, FANG Yong-feng, WANG Han-ning. Effect of exogenous nitric oxide on seedling growth and physiological characteristics of maize seedlings under cadmium stress[J]. Acta Prataculturae Sinica, 2013, 22(2): 178-186.

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