甘肃省嘉峪关市洋葱鳞茎软腐病病原鉴定

doi:10.11686/cyxb20130419

草业学报 ›› 2013, Vol. 22 ›› Issue (4): 153-159.DOI: 10.11686/cyxb20130419

甘肃省嘉峪关市洋葱鳞茎软腐病病原鉴定

吕和平^1,2,魏晖^1,3,漆永红²,张彦梅¹,曹素芳²,杨发荣²,李敏权^2*,沈慧敏^1*

1.甘肃农业大学草业学院,甘肃兰州 730070;
2.甘肃省农业科学院植物保护研究所,甘肃兰州 730070;
3.天水市农业高新技术示范区,甘肃天水 741030

出版日期:2013-08-20 发布日期:2013-08-20
通讯作者: E-mail:ndshm@gsau.edu.cn, lmq@gsau.edu.cn
作者简介:吕和平(1965-),男,山东莱西人,研究员,在读博士。E-mail:Lheping@qq.com
基金资助:
嘉峪关洋葱根腐病研究与防治示范项目(036-036034)资助。

Pathogen identification from soft rot disease of onion bulbs in Jiayuguan, Gansu province

LV He-ping^1,2, WEI Hui^1,3, QI Yong-hong², ZHANG Yan-mei¹, CAO Su-fang²,
YANG Fa-rong², LI Min-quan², SHEN Hui-min¹

1.Grassland College, Gansu Agricultural University, Lanzhou 730070, China;
2.Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;
3.Tianshui Pilot Area of Agricultural Innovative and High Technology, Tianshui 741030, China

Online:2013-08-20 Published:2013-08-20

摘要/Abstract

摘要： 结合形态学与分子生物学特征对甘肃省嘉峪关市洋葱鳞茎软腐病病原种类进行了鉴定,在KB培养基上对两株典型菌株的菌落形态和培养特征进行了观察和描述,16S rDNA序列测定和同源性比对结果表明两菌株的16S rDNA序列分别与GenBank数据库已知胡萝卜软腐果胶杆菌胡萝卜亚种和格氏沙雷氏菌的序列同源性均达99%,其片段大小分别为1 386 bp和1 379 bp。致病性测定结果表明,2种菌均能引起洋葱鳞茎软腐病,且洋葱基部的发病程度高于顶部,其中胡萝卜软腐果胶杆菌胡萝卜亚种的致病力强于格氏沙雷氏菌。按柯赫氏法则初步确定甘肃省嘉峪关市洋葱鳞茎软腐病病原种类为胡萝卜软腐果胶杆菌胡萝卜亚种和格氏沙雷氏菌2种,由格氏沙雷氏菌引起的洋葱鳞茎软腐病属首次报道。本研究将为洋葱软腐病的防治提供理论依据。

Abstract: A severe soft rot disease of onion (Allium cepa) bulbs occurred in Jiayuguan, Gansu province. The causal agent was isolated and identified based on morphological characteristics of two strains on KB culture medium and on molecular characters. The 16S rDNA region of the two strains had homoeology with the known species Serratia grimesii and Pectobacterium carotovorum subsp. carotovorum from the GenBank database. The sequence lengths were 1 379 bp and 1 386 bp, respectively with a maximum similarity of 99％and 100%. Both strains caused soft rot disease on onion bulbs in pathogenicity tests. The disease index from roots was higher than that from the tops and P. carotovorum subsp. carotovorum was more pathogenic than S. grimesii. The pathogens causing soft rot disease on onion bulbs in Jiayuguan, Gansu province were P. carotovorum subsp. carotovorum and S. grimesii. This is the first report of S. grimesii causing soft rot disease on onion bulbs and this study should provide a theoretical basis for controlling this disease.

中图分类号:

吕和平,魏晖,漆永红,张彦梅,曹素芳,杨发荣,李敏权,沈慧敏. 甘肃省嘉峪关市洋葱鳞茎软腐病病原鉴定[J]. 草业学报, 2013, 22(4): 153-159.

LV He-ping, WEI Hui, QI Yong-hong, ZHANG Yan-mei, CAO Su-fang, YANG Fa-rong, LI Min-quan, SHEN Hui-min. Pathogen identification from soft rot disease of onion bulbs in Jiayuguan, Gansu province[J]. Acta Prataculturae Sinica, 2013, 22(4): 153-159.

参考文献

唐成顺. 对嘉峪关市洋葱产业发展的思考. 蔬菜栽培, 2007, 8: 13.
Vandamme P, Pot B, Gillis M, et al. Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiological Reviews, 1996, 60: 407-438.
Taghavi M, Hayward C, Sly L I, et al. Analysis of the phylogenetic relationships of strains of Burkholderia solanacearum,Pseudomonas syzygii, and the blood disease bacterium of banana based on 16S rRNA gene sequence. International Journal of Systematic Bactedlogy, 1996, 46: 10-15.
刘长远, 赵奎华, 傅俊范, 等. 黄瓜细菌性白枯病CU-PV 07菌株鉴定及16S rDNA序列分析. 沈阳农业大学学报, 2008, 39(6): 682-685.
杨瑞先. 利用16S rDNA序列分析鉴定油菜内生细菌研究. 杨凌: 西北农林科技大学, 2005.
邓刚, 屈星, 陈秀蓉, 等. 甘肃番茄细菌性斑点病病原菌鉴定. 植物保护, 2008, 34(5): 47-51.
王霞, 陈哲, 袁红朝, 等. 应用16S rDNA克隆文库技术研究长期稻草还田对水稻土细菌多样性的影响. 生态学报, 2010, 30(14): 3865-3874.
方中达. 植病研究方法(第3版).北京: 中国农业出版社, 1996.
东秀珠, 蔡妙英. 常见细菌系统鉴定手册. 北京:科学出版社, 2001: 399-417.
奥斯伯F. 精编分子生物学指南. 颜子颖, 王海林, 译.北京: 科学出版社, 2001: 39.
王旭, 曾昭海, 朱波, 等. 燕麦与箭筈豌豆不同混作模式对根际土壤微生物数量的影响. 草业学报, 2009, 18(6): 151-157.
赵有翼, 蔡立群, 王静, 等. 不同保护性耕作措施对三种土壤微生物氮素类群数量及其分布的影响. 草业学报, 2009, 18(4): 125-130.
荣丽, 李贤伟, 朱天辉, 等. 光皮桦细根与扁穗牛鞭草草根分解的土壤微生物数量及优势类群. 草业学报, 2009, 18(4): 117-124.
Olsen G J, Larsen N, Woese C R. The ribosomal database project. Nucleic Acids Research, 1997, 25: 109-110.
Roberts P. A soft rot of imported onions caused by Pseudomonas gladioli. Plant Pathology, 1973, 12(4): 22-98.
Bazzi C. Identification of Pseudomonas cepacia on onion bulbs in Italy. Phytopathology, 1979, 95: 254-258.
Gitaitis R D, Baird R E. Bacterial blight of sweet onion caused by Pseudomonas viridiflava. Plant Disease, 1991, 75: 1180-1182.
Alvarez A M, Buddenhagen I W, Buddenhagen E S. Bacterial blight of onion, a new disease caused by Xanthomonas campestris. Phytopathology, 1978, 68: 1132-1136.
Schwartz H F, Mohan S K. Compendium of Onion and Garlic Disease. American: the American Phytopathological Society Press, 1996: 32.
任欣正. 植物病原细菌的分类和鉴定. 北京: 农业出版社, 1994: 1-240.
贾云鹤, 张俊华, 崔崇士. 软腐欧文氏菌致病性的研究进展. 东北农业大学学报, 2007, 38(1): 113-118.
王敏, 姬广海, 修建华, 等. 云南省马蹄莲细菌性软腐病原鉴定. 西南大学学报, 2007, 29(8): 79-82.
罗炎华, 鲁红学, 黄萍. 魔芋软腐病研究进展. 农村经济与科技, 2008, 19(5): 89-90.
何煜波. 三叶半夏病毒病和细菌病的鉴定, 检测和侵染特性及脱毒组培研究. 长沙: 湖南农业大学, 2006.
赵玲, 柴兆祥, 李金华, 等. 四株胡萝卜软腐欧文氏杆菌胡萝卜亚种新菌株的分离鉴定. 草业学报, 2011, 20(4): 244-251.
廖咏梅, 张桂英, 黄定安, 等. 甘蔗黑穗病菌拮抗细菌的筛选及鉴定. 广西农业科学, 2004, 23(3): 197-201.
马迎新, 刘晓光, 高克祥, 等. 根际细菌Serratia plymuthica的生防作用初探.云南大学学报, 2007, 22(1): 49-53.
张敏, 沈德龙, 饶小莉, 等. 甘草内生细菌多样性研究. 微生物学通报, 2008, 35(4): 524-528.
Weller D M. Biological control of soil-borne plant-pathogens in the rhizosphere with bacteria. Annual Review of Phytopathology, 1988, 26: 379-407.
Press C M, Wolon M, Tuzum S, et al. Salicylic acid produced by Serratia marcescens 90-166 is not the primary determinant of induced systemic resistance incucumber or tobacoo. Molecular Plant-Microbe Interactions, 1997, 10: 761-768.
Kamensk Y M, Ovadi S M, Cheti, et al. Soil- borne strain IC14 of Serratia plymuthica with multiple mechanisms of antifungal activity provides biocontrol of Botrytis cinerea and Sclerotinia sclerotiorum diseases. Soil Biology Biochemistry, 2003, 35: 323-331.

甘肃省嘉峪关市洋葱鳞茎软腐病病原鉴定

Pathogen identification from soft rot disease of onion bulbs in Jiayuguan, Gansu province

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics