中型狼尾草幼苗对PEG、低温和盐胁迫的生理应答

doi:10.11686/cyxb20140222

草业学报 ›› 2014, Vol. 23 ›› Issue (2): 180-188.DOI: 10.11686/cyxb20140222

中型狼尾草幼苗对PEG、低温和盐胁迫的生理应答

张怀山^1,2,赵桂琴^1*,栗孟飞³,夏曾润⁴,王春梅²

1.甘肃农业大学草业学院,甘肃兰州 730070;
2.中国农业科学院兰州畜牧与兽药研究所农业部兰州黄土高原生态环境重点野外科学观测试验站,甘肃兰州 730050;
3.甘肃农业大学生命科学技术学院干旱生境作物学重点实验室,甘肃兰州 730070;
4.兰州大学草地农业科技学院草地农业生态系统国家重点实验室,甘肃兰州 730020

收稿日期:2013-02-21 出版日期:2014-02-25 发布日期:2014-04-20
通讯作者: E-mail:zhaogq@gsau.edu.cn
作者简介:张怀山(1969-),男,甘肃兰州人,在读博士。E-mail:zhanglz2007@163.com
基金资助:
国家自然科学基金项目(31201841), 甘肃省农业科技创新项目(GNCX-2013-58)和兰州市科技计划项目(2011-1-167)资助。

Physiological responses of Pennisetum longissimum var. intermedium seedlings to PEG, low temperature and salt stress treatments

ZHANG Huai-shan^1,2, ZHAO Gui-qi¹, LI Meng-fei³, XIA Zeng-run⁴, WANG Chun-mei²

1.College of Pratacultural Science, Gansu Agriculture University, Lanzhou 730070, China;
2.Lanzhou Institute of Husbandry and Pharmaceutical Sciences CAAS, The Lanzhou Scientific Observation and Experiment Field Station of Ministry of Agriculture for Ecological System in Loess Plateau Areas, Lanzhou 730050, China;
3.College of Life Science and Technology, Gansu Agricultural University, Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070, China;
4.College of Pastoral Agriculture Science and Technology, Lanzhou University, State Key Laboratory of Grassland Agro-ecosystems, Lanzhou 730020, China

Received:2013-02-21 Online:2014-02-25 Published:2014-04-20

摘要/Abstract

摘要： 采用PEG-6000、低温和NaCl处理模拟逆境胁迫的方法,系统研究了中型狼尾草幼苗在3种不同胁迫条件下的生理生化特性的变化,并对其抗逆性、各生理生化指标的相关性进行了综合评价与分析。结果显示,1)随着PEG浓度的增加,可溶性蛋白含量表现为先增后降再增的趋势;可溶性糖含量呈现先增后降,在PEG 5%时达到最大;脯氨酸含量显著升高,在PEG 20%时含量是CK的6.0倍;MDA含量呈现“急剧增加—相对稳定—迅速下降”的变化趋势,PEG 5%时MDA累积量达到最大;SOD活性总体上呈“急剧下降—微增—迅速上升”的趋势,PEG 5%时最低,PEG 20%时达到最大。2)低温胁迫过程中,可溶性蛋白和可溶性糖含量均随温度降低总体呈现先升高后下降的趋势;脯氨酸含量随温度的降低均呈升高趋势;MDA含量随低温处理时间的延长而增大;SOD活性随温度的降低总体呈现先升高后下降的趋势。3) 随着NaCl胁迫水平的加强,可溶性蛋白含量表现为先增后降的变化趋势;可溶性糖、脯氨酸和MDA含量均随着NaCl浓度的增大而增加;SOD活性则呈现“微增—缓慢降低—急剧下降”的变化趋势。相关性分析结果显示:PEG胁迫下,脯氨酸含量与可溶性糖含量呈显著负相关;低温4 h胁迫下,MDA含量与可溶性糖含量显著正相关,与脯氨酸含量极显著正相关;NaCl胁迫下,可溶性糖含量与脯氨酸含量和MDA含量极显著正相关,SOD活性与可溶性糖含量、MDA含量呈显著负相关,而与可溶性蛋白显著正相关。以上结果表明,中型狼尾草适应逆境胁迫受到多种生理生化的调节,且各生理生化物质之间存在一定的相关性,其生理指标的动态变化是中型狼尾草应答逆因子胁迫的重要生理调节机制,体现了其抵抗逆境胁迫、减轻伤害的适应性反应。

Abstract: The physiological characteristics of Pennisetum longissimum var. intermedium seedlings under three treatments of PEG-6000, low temperature and salt stress were studied using simulation adversity stress methods. The resistance and ecological adaptability were analyzed and comprehensively evaluated: 1) With increased concentrations of PEG the soluble proteins content initially increased but then decreased before increasing again. Soluble sugar initially increased but then decreased and had a maximal value when PEG was 5%. The content of MDA sharply increased at first, then remained stable and finally decreased significantly after a maximal value when PEG was 5%. The activity of SOD sharply decreased at first, then increased slowly then increased significantly. It had a minimal value when PEG was 5% and a maximal value with 20% PEG. 2) The contents of soluble protein and soluble sugar and the activity of SOD increased at first but then decreased with low temperature stress. The content of proline increased with increasing temperatures and MDA also showed an increasing trend. 3) With increased concentrations of salt, the soluble protein content initially increased but then decreased while the contents of soluble sugar, proline and MDA increased. SOD activity showed a slight increase at first, then slowly reduced followed by a rapid decline. Correlation analysis showed that the content of proline was significantly negatively correlated with soluble sugar concentration under PEG treatment. With low temperature stress of 4 hours, the contents of MDA were significantly positively correlated with soluble sugar and proline. With NaCl treatment, the content of soluble sugar was significantly positively correlated with MDA and proline, the activity of SOD was significantly negatively correlated with soluble sugar and MDA while it was significantly positively correlated with soluble protein. To sum up, the physiological dynamic changes of P. longissimum var. intermedium in its response to inverse factor stress reflects its adaptive response of resistance to adversity stresses and reducing injury.

中图分类号:

张怀山,赵桂琴,栗孟飞,夏曾润,王春梅. 中型狼尾草幼苗对PEG、低温和盐胁迫的生理应答[J]. 草业学报, 2014, 23(2): 180-188.

ZHANG Huai-shan, ZHAO Gui-qi, LI Meng-fei, XIA Zeng-run, WANG Chun-mei. Physiological responses of Pennisetum longissimum var. intermedium seedlings to PEG, low temperature and salt stress treatments[J]. Acta Prataculturae Sinica, 2014, 23(2): 180-188.

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中型狼尾草幼苗对PEG、低温和盐胁迫的生理应答

Physiological responses of Pennisetum longissimum var. intermedium seedlings to PEG, low temperature and salt stress treatments

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