生长期内三种结缕草对盐胁迫的响应

doi:10.11686/cyxb2015161

摘要/Abstract

摘要： 试验以大穗结缕草、沟叶结缕草和日本结缕草为试材,研究了不同盐浓度(0,0.2%,0.4%,0.6%,0.8%)对生长期内3种结缕草叶片膜脂过氧化及生长的影响。结果表明,3种结缕草的生物量均随着盐浓度的增加逐渐减小;随着盐浓度的提高及处理时间的延长,3种结缕草的可溶性蛋白含量逐渐降低;相对电导率和丙二醛含量增加,在低盐浓度(0.2%~0.4%)下,两者增加幅度较小,当盐浓度为0.8%时,3种结缕草的相对电导率均达最大值,分别是38.71%,41.32%,40.97%,可溶性蛋白含量均达到最小值,分别为2.08,1.15和1.33 mg/g FW。Pro含量在处理后第10天达最大值,之后逐渐下降,后期变化比较平稳。在低盐浓度(0.2%~0.4%)下,CAT和SOD活性逐渐降低,而高盐浓度(0.6%~0.8%)下,两种酶活性呈上升-下降-上升的趋势。3种结缕草相比,大穗结缕草在高盐浓度胁迫下的相对电导率和丙二醛含量最低,而保护酶活性和可溶性蛋白含量最高。试验证明大穗结缕草抗盐能力最强,沟叶结缕草次之,日本结缕草较差。

Abstract: The effects of salt (NaCl) on the antioxidant system and soluble protein content during growth of Zoysia macrostachys, Z. matrella and Z. japonica was investigated. The biomass and the soluble protein contents of all three grasses reduced gradually with increasing salt concentration and duration of exposure, while leaf relative conductance and malondialdehyde (MDA) content increased. Under lower salt concentration (0.2%-0.4%), leaf relative conductance and MDA increased slightly, but both peaked at 0.8% salt. The proline content peaked 10 days after treatment application but subsequently declined. Under lower salt concentrations the activities of catalase and superoxide dismutase in all three grasses reduced gradually, while under 0.6%-0.8% salt, they initially increased, declined and then increased again. Z. macrostachys had the lowest leaf relative conductance and the lowest MDA, but the highest protective enzyme activity and soluble protein content. Consequently Z. macrostachys demonstrated greater salt resistance than Z. matrella and Z. japonica.

赵莹, 李静媛, 段艳欣, 董晓颖. 生长期内三种结缕草对盐胁迫的响应[J]. 草业学报, 2015, 24(11): 109-117.

ZHAO Ying, LI Jing-Yuan, DUAN Yan-Xin, DONG Xiao-Ying. Responses of three Zoysia grass species to salt stress[J]. Acta Prataculturae Sinica, 2015, 24(11): 109-117.

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