转DREB1A/Bar双价基因马铃薯的耐旱性及除草剂抗性分析

doi:10.11686/cyxb2014533

草业学报 ›› 2015, Vol. 24 ›› Issue (11): 58-64.DOI: 10.11686/cyxb2014533

转DREB1A/Bar双价基因马铃薯的耐旱性及除草剂抗性分析

贾小霞^{1, 2, *}, 齐恩芳^{1, 2}, 马胜^{1, 2}, 胡新元^{1, 2}, 王一航^{1, 2}, 文国宏^{1, 2}, 龚成文^{1, 2}, 李建武^{1, 2}

1.甘肃省农业科学院马铃薯研究所,甘肃省马铃薯种质资源创新工程实验室,甘肃兰州 730070; 2.农业部西北旱作马铃薯科学观测实验站,甘肃渭源 748201

收稿日期:2014-12-19 出版日期:2015-11-20 发布日期:2015-11-20
通讯作者: E-mail:jiaxx0601@163.com
作者简介:贾小霞(1978-),女,甘肃定西人,副研究员,博士。
基金资助:
甘肃省自然科学基金(145RJZA088),国家自然科学基金(31060200),甘肃省农业科学院中青年基金(2014GAAS20)和国家马铃薯产业技术体系(CARS-10-P05)资助

Analysis of drought tolerance and herbicide resistance in transgenic potato plants over-expressing DREB1A/Bar

JIA Xiao-Xia^{1, 2, *}, QI En-Fang^{1, 2}, MA Sheng^{1, 2}, HU Xin-Yuan^{1, 2}, WANG Yi-Hang^{1, 2}, WEN Guo-Hong^{1, 2}, GONG Cheng-Wen^{1, 2}, LI Jian-Wu^{1, 2}

1.Potato Research Institute, Gansu Academy of Agricultural Sciences, Gansu Engineering Laboratory of Potato Germplasm Resources Innovation, Lanzhou 730070, China; 2.The Ministry of Agriculture, Scientific Observation and Experiment Station of Dry potato in the Northwest, Weiyuan 748201, China

Received:2014-12-19 Online:2015-11-20 Published:2015-11-20

摘要/Abstract

摘要： 在前期获得DREB1A/Bar双价转基因马铃薯的基础上,对转基因植株进行了耐旱性和除草剂抗性分析。耐旱性分析显示,在正常浇水条件下,对照和各转基因马铃薯株系生长状态良好且大致相同,各株系的丙二醛含量、相对电导率和SOD酶活性无显著差异(P>0.05)。经过控水10 d后,非转基因对照植株叶片明显萎蔫卷曲,而转基因植株仍然保持良好的生长状态;转基因株系的丙二醛含量和相对电导率显著低于非转基因株系(P<0.05),而SOD酶活性显著高于非转基因对照(P<0.05)。控水18 d时,大部分对照植株死亡,死亡率为74.33%;转基因植株只有极少数植株死亡,DR2和DR5的死亡率分别为20.43%和5.65%。用0.3%的市售草铵膦喷施各株系,10 d后,对照植株全部枯死,转基因株系的个别叶片干枯,绝大多数叶片及所有茎秆生长状态良好。以上分析表明,DREB1A和Bar基因的导入,明显增强了转基因马铃薯对干旱和除草剂的抗性。

Abstract: In order to compare drought resistance of DREB1A/Bar transgenic potato plants, with a control cultivar (non-transgenic Longshu 10), 5-7 cm seedlings of DREB1A transgenic lines were grown in pots using vermiculite and regularly watered. Watering continued to the 15-16 leaf stage after which plants were not watered for 18 days to impose for drought stress. During the drought stress, plant phenotypic features were recorded using visual observation and digital camera images. After 10 days of drought stress, stress-related physiological and biochemical parameters were determined. During the normal watering phase both the non-transgenic control and the transgenic potato lines grew well, the MDA content, relative electrical conductivity and superoxide dismutase (SOD) activity were not significantly different (P>0.05). After 10 days of drought stress the control plants began to wilt, but the transgenic lines remained in good condition. MDA content and relative electrical conductivity in the transgenic line was significantly lower than those of the non-transgenic plants (P<0.05), while the SOD activity was significantly higher than that of non-transgenic plants (P<0.05). After for 18 days of drought stress 74.33% of the control plants were dead whereas fewer transgenic plants had died; the mortality of DR2 and DR5 was 20.43% and 5.65% respectively. The resistance of DREB1A/Bar transgenic potatoes to glufosinate, a systemic non-selective herbicide, compared with non-transgenic lines was determined by treating plants with a 0.3% glufosinate spray. Ten days after treatment all non-transgenic plants had died, while the transgenic plants were affected slightly. The study indicated that introduction of DREB1A and Bar genes significantly enhanced drought tolerance and herbicide resistance in transgenic potato plants.

贾小霞, 齐恩芳, 马胜, 胡新元, 王一航, 文国宏, 龚成文, 李建武. 转DREB1A/Bar双价基因马铃薯的耐旱性及除草剂抗性分析[J]. 草业学报, 2015, 24(11): 58-64.

JIA Xiao-Xia, QI En-Fang, MA Sheng, HU Xin-Yuan, WANG Yi-Hang, WEN Guo-Hong, GONG Cheng-Wen, LI Jian-Wu. Analysis of drought tolerance and herbicide resistance in transgenic potato plants over-expressing DREB1A/Bar[J]. Acta Prataculturae Sinica, 2015, 24(11): 58-64.

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转DREB1A/Bar双价基因马铃薯的耐旱性及除草剂抗性分析

Analysis of drought tolerance and herbicide resistance in transgenic potato plants over-expressing DREB1A/Bar

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