甘肃省张掖地区苜蓿花叶病病原的检测

doi:10.11686/cyxb20150414

草业学报 ›› 2015, Vol. 24 ›› Issue (4): 121-126.DOI: 10.11686/cyxb20150414

甘肃省张掖地区苜蓿花叶病病原的检测

文朝慧^1,2,南志标^1*

1.兰州大学草地农业科技学院,草地农业生态系统国家重点实验室,甘肃兰州 730020;
2.甘肃出入境检验检疫局,甘肃兰州 730010

收稿日期:2013-10-11 修回日期:2013-12-16 出版日期:2015-04-20 发布日期:2015-04-20
通讯作者: 南志标,E-mail:zhibiao@lzu.edu.cn
作者简介:文朝慧(1969-),女,四川简阳人,高级农艺师,在读博士。E-mail:wzhhli@163.com
基金资助:
公益性行业(农业)科技专项(No.201303057),国家质检总局科技计划项目(2012IK270)和甘肃出入境检验检疫局科技计划项目(2011GK001)资助。

Detection of pathogenic organisms in Medicago sativa in Zhangye, Gansu Province

WEN Zhao-Hui^1,2, NAN Zhi-Biao^1*

1.College of Pastoral Agricultural Science and Technology, State Key Laboratory of Grassland Agro-Ecosystems, Lanzhou University, Lanzhou 730020, China;
2.Gansu Exit-Entry Inspection and Quarantine Bureau, Lanzhou 730010, China

Received:2013-10-11 Revised:2013-12-16 Online:2015-04-20 Published:2015-04-20

摘要/Abstract

摘要： 2011年7月在甘肃省张掖地区发现发生花叶病的苜蓿田块,地块中病株呈现黄斑花叶、叶柄扭曲及整株矮化的症状。为明确其病原,采集病株后利用血清学和分子生物学方法对病样进行了检测。 DAS-ELISA、RT-PCR-RFLP及病毒CP基因序列测定分析结果表明,苜蓿病样受到番茄花叶病毒(Tomato mosaic virus,ToMV)和苜蓿花叶病毒(Alfalfa mosaic virus,AMV)的复合侵染。这是国际上首次报道番茄花叶病毒对苜蓿的侵染,讨论了由这两种病毒引致病害的发生与防治。

Abstract: Two Medicago sativa plants with severe leaf mosaic symptoms were collected from alfalfa fields in Linze County, Gansu Province, in July 2011. The two samples were tested by enzyme-linked-immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR). The tested specimens were negative for Cucumber mosaic virus, Tobacco streak virus, Watermelon mosaic virus, Potato virus X, Potato virus Y, Tobacco ringspot virus, Tomato ringspot virus and Bean yellow mosaic virus. However, Tomato mosaic virus (ToMV) and Alfalfa mosaic virus (AMV) were detected. The 687 and 351 bp fragments obtained from PCR with appropriate species-specific ToMV and AMV primers showed 99% nucleotide similarity with published ToMV CP and AMV CP gene sequences. A 805 bp PCR products was obtained using genus-specific Tobamovirus primers and the presence of ToMV was confirmed by RT-PCR-RFLP. To the best of our knowledge, this is the first report of ToMV in alfalfa.

文朝慧,南志标. 甘肃省张掖地区苜蓿花叶病病原的检测[J]. 草业学报, 2015, 24(4): 121-126.

WEN Zhao-Hui, NAN Zhi-Biao. Detection of pathogenic organisms in Medicago sativa in Zhangye, Gansu Province[J]. Acta Prataculturae Sinica, 2015, 24(4): 121-126.

参考文献

Geng H Z, Wu Y F, Cao Z Z. Alfalfa in China. Beijing: China Agriculture Press, 1995: 1-50.
Xie K Y, Zhao Y, Li X L, et al. Relationship between grasses and legumes in mixed glassland: a review. Acta Prataculturae Sinica, 2013, 22(3): 284-296.
Yang S Q, Sun Z M, Yang X P. Developing alfalfa industrialization to promote agricultural structure adjustment in the northwest arid area of China. Acta Agriculturae Boreali-occidentalis Sinica, 2003, 12(4): 157-160.
Cao L, Qin S H, Zhang J L, et al. Effect of leguminous forage rotations on soil microbe consortiums and enzyme activity in continuously cropped potato fields. Acta Prataculturae Sinica, 2013, 22(3): 139-145.
Ren J Z. Establishment of an agro-grassland systems for grain storage——A thought on restructure of agricultural framework in Western China. Acta Prataculturae Sinica, 2002, 11(1): 1-3.
Freeman A J, Aftab M. Effective management of viruses in pulse crops in south eastern Australia should include management of weeds. Australasian Plant Pathology, 2011, 40: 430-441.
Stuteville D L, Erwin D C (ed.). Compendium of Alfalfa Diseases, 2nd edn. St. Paul, MN, USA: APS Press, 1990: 51-53.
Cai F X, Mang K Q. Study of alfalfa mosaic virus isolate H-10 I. The biological identification, purification and physical, chemical properties. Acta Microbiologica Sinica, 1982, 22(3): 233-240.
Zhang X L, Yin Y Q, Li G Y, et al. Study on bean yellow mosaic virus isolated from alfalfa. Virologica Sinica, 1990, (1): 88-96.
Zhang X L, Yin Y Q, Cui X M, et al. Studies of Cowpea mosaic virus on alfalfa. Acta Phytopathologica Sinica, 1994, 24(1): 69-75.
Shang W J. Alfalfa leaf disease survey and pathogen identification in Ningxia. Pratacultural Science, 1997, 14(1): 23-25.
Wang X W, Yu N L, Ma D C.The preliminary study of the types and distributions of lucerne disease in Xinjiang. Acta Prataculturae Sinica, 1998, 7(2): 48-52.
Nan Z B. Lucerne diseases found in China and their integrated management system. Animal Science and Veterinary Medicine, 2001, 18(4): 1-4.
Clark M F, Adams A N. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 1977, 34(3): 475-485.
Letschert B, Adam G, Lesemann D E, et al. Detection and differentiation of serologically cross-reacting tobamoviruses of economical importance by RT-PCR and RT-PCR-RFLP. Journal of Virological Methods, 2002, 106: 1-10.
Xu H, Nie J. Identification, characterization and molecular detection of Alfalfa mosaic virus in potato. Phytopathology, 2006, 96: 1237-1242.
Hammond J, Hammond R W. Molecular cloning, sequencing and expression in Escherichia coli of the bean yellow mosaic virus coat protein gene. Journal of General Virology, 1989, 70: 1961-1974.
Strasser M, Pfitzner A J. The double-resistance-breaking Tomato mosaic virus strain ToMV1-2 contains two independent single resistance-breaking domains. Archives of Virology, 2007, 152(5): 903-914.
Parrella G, Acanfora N, Bellardi M G. First record and complete nucleotide sequence of Alfalfa mosaic virus from Lavandula stoechas in Italy. Plant Disease, 2010, 94(7): 924-924.
Hemmati K, Mclean D L. Gamete-seed transmission of alfalfa mosaic virus and its effect on seed germination and yield of alfalfa plants. Phytopathology, 1977, 67: 576-579.
Ohki S T, Leps W T, Hiruki C. Effects of alfalfa mosaic virus infection on factors associated with symbiotic N₂ fixation in alfalfa. Canadian Journal of Plant Pathology, 1986, 8: 277-281.
Tu J C, Holmes T M. Effect of alfalfa mosaic virus infection on nodulation, forage yield, forage protein and over wintering of alfalfa. Journal of Phytopathology, 1980, 97(1): 1-9.
Hollings M, Huttinga H. Tomato Mosaic Virus. Descriptions of Plant Viruses. Kew, England: Commonwealth Mycology Institute/Association for Applied Biology, 1976.
Brunt A A. Tomato Mosaic Virus. In: Van Regenmortel M H V, Fraenkel-Conrat H, eds. The Plant Viruses. Vo1. 2. New York: Plenum Press, 1986: 181-203.
Zhou X P, Qian X H, Liu Y, et al. Studies of Tomato mosaic virus on tomato. Virologica Sinica, 1996, 1(3): 268-276.
Zhou X P, Xue Z Y, Liu Y, et al. Biology and serological comparison of ToMV tomato isolate and TMV broad bean isolate and specific detection with polymerase chain reaction. Acta Phytopathologica Sinica, 1997, 27(1): 53-58.
Yu C, Wu J X, Zhou X P. Production of monoclonal antibodies to Tomato mosaic virus and application in virus detection. Acta Microbiologica Sinica, 2002, 42(4): 453-457.
Yuan Q H. Advances in alfalfa diseases in China. Plant Protection, 2007, 33(1): 6-10.
Hull R. Matthews’ Plant Virology, 4th edn. Beijing: Science Press, 2007: 736.
Wen Z H, Liu Y L, Liu Q, et al. Identification of viruses infecting tomato in Hexi region of Gansu Province. Acta Agriculturae Boreali-occidentalis Sinica, 2009, 18(6): 291-294.
耿华珠, 吴永敷, 曹致中. 中国苜蓿. 北京: 中国农业出版社, 1995: 1-50.
谢开云, 赵云, 李向林, 等. 豆－禾混播草地种间关系研究进展.草业学报, 2013, 22(3): 284-296.
杨世琦, 孙兆敏, 杨雄平. 发展苜蓿草产业促进西北干旱地区农业结构调整. 西北农业学报, 2003, 12(4): 157-160.
曹莉, 秦舒浩, 张俊莲, 等. 轮作豆科牧草对连作马铃薯田土壤微生物菌群及酶活性的影响. 草业学报, 2013, 22(3): 139-145.
任继周. 藏粮于草施行草地农业系统——西部农业结构改革的一种设想.草业学报, 2002, 11(1): 1-3.
蔡发兴, 莽克强. 北京苜蓿花叶病毒H-10分离物的研究-Ⅰ.病毒的生物学测定、提纯及其理化性质. 微生物学报, 1982, 22(3): 233-240.
张祥林, 尹玉琦, 李国英, 等. 菜豆黄色花叶病毒(BYMV)-新疆苜蓿分离株的鉴定. 病毒学杂志, 1990, (1): 88-96.
张祥林, 尹玉琦, 崔星明, 等. 侵染苜蓿的豇豆花叶病毒的研究. 植物病理学报, 1994, 24(1): 69-75.
商文静. 宁夏紫花苜蓿叶部病害调查和病原菌鉴定. 草业科学, 1997, 14(1): 23-25.
王雪薇, 喻宁莉, 马德成. 新疆苜蓿病害的种类和分布的初步研究. 草业学报, 1998, 7(2): 48-52.
南志标. 我国的苜蓿病害及其综合防治体系. 动物科学与动物医学, 2001, 18(4): 1-4.
周雪平, 钱秀红, 刘勇, 等. 侵染番茄的番茄花叶病毒的研究. 中国病毒学, 1996, 1(3): 268-276.
周雪平, 薛朝阳, 刘勇, 等. 番茄花叶病毒番茄分离物与烟草花叶病毒蚕豆分离物生物学、血清学比较及PCR特异性检测.植物病理学报, 1997, 27(1): 53-58.
于翠, 吴建祥, 周雪平. 番茄花叶病毒单克隆抗体的制备及检测应用. 微生物学报, 2002, 42(4): 453-457.
袁庆华. 我国苜蓿病害研究进展. 植物保护, 2007, 33(1): 6-10.
赫尔R. 马修斯植物病毒学(原书第4版). 北京: 科学出版社, 2007: 736.
文朝慧, 刘雅莉, 刘箐, 等. 甘肃河西地区番茄病毒病病原种类鉴定. 西北农业学报, 2009, 18(6): 291-294.

甘肃省张掖地区苜蓿花叶病病原的检测

Detection of pathogenic organisms in Medicago sativa in Zhangye, Gansu Province

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics