淹水对紫花苜蓿南北方品种抗氧化酶和无氧呼吸酶的影响

doi:10.11686/cyxb20150510

草业学报 ›› 2015, Vol. 24 ›› Issue (5): 84-90.DOI: 10.11686/cyxb20150510

淹水对紫花苜蓿南北方品种抗氧化酶和无氧呼吸酶的影响

全瑞兰^{1, 2}, 玉永雄^{1*, *}

1.西南大学动物科技学院,重庆 400716;
2.信阳市农业科学院,河南信阳 464000

收稿日期:2014-03-11 出版日期:2015-05-20 发布日期:2015-05-20
作者简介:全瑞兰(1985-),女,河南平顶山人,硕士。E-mail:qrlhe@163.com
基金资助:
973计划课题(2007CB108901),948项目(2014-Z31)和国家“十一五”支撑计划项目(2011BAD36B02,2011BAD36B03)资助

Effect of waterlogging on antioxidant and anaerobic respiratory enzymesin Medicago sativa varieties from southern and northern China

QUAN Rui-Lan^{1, 2}, YU Yong-Xiong^{1, *}

1.College of Animal Science and Technology, Southwest University, Chongqing 400716, China;
2.Xinyang City Academy of Agricultural Sciences, Xinyang 464000, China

Received:2014-03-11 Online:2015-05-20 Published:2015-05-20

摘要/Abstract

摘要： 以紫花苜蓿南方育成品种渝苜1号和北方地方品种新疆紫泥泉为材料,在幼苗长出4片真叶时进行淹水处理,在淹水的第10天测定植株的生物量,并分别在淹水的第0,2,4,6,8,10天测定叶片中的丙二醛(MDA)含量以及叶片抗氧化酶和根系无氧呼吸酶的活性,以探明两个品种对淹水胁迫的耐受性差异及其生理响应机制。结果表明,淹水胁迫使紫花苜蓿的生物量减少,但与新疆紫泥泉相比,渝苜1号生物量的降幅小。淹水时渝苜1号叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)等抗氧化酶活性比新疆紫泥泉的高,而叶片MDA含量比新疆紫泥泉的低;根系无氧呼吸酶活性在淹水胁迫中均增强,但渝苜1号的乙醇脱氢酶(ADH)和丙酮酸脱羧酶(PDC)活性增加明显,乙醇发酵更强;而新疆紫泥泉的乳酸脱氢酶(LDH)的活性增加更为突出,乳酸发酵强。淹水影响紫花苜蓿正常生长,但南方品种渝苜1号比北方品种新疆紫泥泉对淹水胁迫更具耐受性,因为前者具有比较高的抗氧化酶活性和比较强的乙醇发酵途径,有利于增强植株抗淹水胁迫能力。

Abstract: Two Medicago sativa varieties, Yumu No.1 (southern China) and Xinjiangziniquan (northern China), were compared. Six waterlogging treatments; 0, 2, 4, 6, 8 and 10 days of waterlogging were imposed on seedlings at the 4-leaf stage. The malondialdehyde (MDA) content, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity in the leaves and lactate dehydrogenase (LDH), pyruvate (PDC) and alcohol dehydrogenase (ADH) activity in the roots were evaluated in all treatments. Biomass was measured after 10 days of waterlogging. Waterlogging stress reduced the biomass of both varieties but Yumu No.1 was less affected than Xinjiangziniquan. The activities of SOD, POD and CAT in leaves of Yumu No.1 were higher than those of Xinjiangziniquan under waterlogging stress, but the MDA content in the leaves was lower than that of Xinjiangziniquan. The activity of respiratory enzymes in the roots was influenced by waterlogging; In Yumu No.1 ADH and PDC activity increased more than LDH activity with stronger alcohol dehydrogenaseactivity, while in Xinjiangziniquanwaterlogging resulted in increased LDH and lactic dehydrogenase activity. It was concluded that the greater tolerance of waterlogging observed in Yumu No.1 was primarily due to higher antioxidant enzyme and anaerobic respiratory enzyme activity.

全瑞兰, 玉永雄. 淹水对紫花苜蓿南北方品种抗氧化酶和无氧呼吸酶的影响[J]. 草业学报, 2015, 24(5): 84-90.

QUAN Rui-Lan, YU Yong-Xiong. Effect of waterlogging on antioxidant and anaerobic respiratory enzymesin Medicago sativa varieties from southern and northern China[J]. Acta Prataculturae Sinica, 2015, 24(5): 84-90.

参考文献

[1] Liu T. The role of alfalfa in agri-ecological systems. Jilin Animal Husbandry and Veterinary Medicine, 2005, (7): 21-23.
[2] Yue Y H, Qi X, Wang Y R, et al . Persistence of 35 Medicago sativa varieties at the 10th year after establishment. Acta Prataculturae Sinica, 2014, 23(1): 58-64.
[3] He Z Q, Huan H F, He H X, et al . Problems and solutions of the grass industry in south China. Tropical Agricultural Engineering, 2009, 33(3): 83-86.
[4] An Y, Cheng F Y, Wang J, et al . Studies on waterlogging tolerance of semi-fall and non-fall dormant alfalfa cultivars. Grassland of China, 2004, 26(4): 31-36.
[5] Zhou G S, Zhu X T. Changes of physiological characters of wheat after waterlogging and relations between physiological characters and waterlogging tolerance of different varieties. Scientia Agricultura Sinica, 2002, 35(7): 777-783.
[6] Li L, Zou D S, Liu D W, et al . Research progress on waterlogging in peanut and other crops. Chinese Journal of Oil Crop Sciences, 2004, 26(3): 105-110.
[7] Liu W G, Yan Z H, Wang C, et al . Response of antioxidant defense system in watermelon seeding subjected to waterlogged stress. Journal of Fruit Science, 2006, 23(6): 860-864.
[8] Chen L Z, Lin P, Wang W Q. Mechanisms of mangroves waterlogging resistance. Acta Ecologica Sinica, 2006, (2): 586-593.
[9] Ahmed S, Nawata E, Hosokawa M, et al . Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging. Plant Science, 2002, 163(1): 117-123.
[10] Mustroph A, Albrecht G. Tolerance of crop plants to oxygen deficiency stress: fermentative activity and photosynthetic capacity of entire seedlings under hypoxia and anoxia. Acta Physiologiae Plantarum, 2003, 117(4): 508-520.
[11] Ismail A M, Ella E S, Vergara G V, et al . Mechanisms associated with tolerance to flooding during germination and early seedling growth in rice ( Oryza sativa ). Annals of Botany, 2009, 103(2): 197-209.
[12] Kang Y Y, Guo S R, Li J, et al . Effect of root applied 24-epibrassinolide on carbohydrate status and fermentative enzyme activities in cucumber ( Cucumis sativus L.) seedlings under hypoxia. Plant Growth Regulation, 2009, 57: 259-269.
[13] Wang X K. The Principle and Technology of Plant Physiology and Biochemistry Experiment[M]. Beijing: Higher Education Press, 2006: 228-281.
[14] Wang Q F, Yi Q, Hu Q Q, et al . Simultaneous overexpression of citrate synthase and phosphoenolpyruvate carboxylase in leaves augments citrate exclusion and Alresistance in transgenic tobacco. Plant Molecular Biology Reporter, 2012, 30: 992-1005.
[15] Yang J H, Gao Y, Li Y M, et al . Salicylic acid-induced enhancement of cold tolerance through activation of antioxidative capacity in watermelon. Scientia Horticultrae, 2008, 118: 200-205.
[16] Wang K, Bian S, Jiang Y. Anaerobic metabolism in roots of Kentucky bluegrass in response to short-term waterlogging alone and in combination with high temperatures. Plant and Soil, 2009, 314: 221-229.
[17] Li C Y, Bai T H, Ma F W, et al . Hypoxia tolerance and adaptation of anaerobic respiration to hypoxia stress in two Malus species. Scientia Horticulturae, 2010, 124(2): 274-279.
[18] Bradford M M. A rapid and sensitive method for the quantitation of icrogram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 1976, 72: 248-254.
[19] Liu J, Cai H, Liu Y, et al . A study on physiological characteristics and comparison of salt tolerance of two Medicago sativa at the seeding stage. Acta Prataculturae Sinica, 2013, 22(2): 250-256.
[20] Li Y, Liu G B, Gao H W, et al . A comprehensive evaluation of salt-tolerance and the physiological response of Medicago sativa at the seeding stage. Acta Prataculturae Sinica, 2010, 19(4): 79-86.
[21] Wu J, Cai H, Bai X, et al . An analysis of salt tolerance of transgenic alfalfa with the GsGST 13 /SCMRP gene. Acta Prataculturae Sinica, 2014, 23(1): 257-265.
[22] Li Z, Mei S F, Liu X L, et al . Research progress in waterlogging and submergence tolerance of crops. Hubei Agricultural Sciences, 2009, 48(11): 2866-2868.
[23] Nada K, Iwatani E, Doi T, et al . Effect of putrescine pretreatment to roots on growth and lactate metabolism in the root of tomato ( Lycopersicon esculentum Mill.) under root-zone hypoxia. Journal of the Japanese Society for Horticultural Science, 2004, 73(4): 337-339.
[24] Ismond K P, Dolferus R, Pauw M, et al . Enhanced lowoxygen survival in Arabidopsis through increased metabolic flux in the fermentative pathway. Plant Physiology, 2003, 132(3): 1292-1302.
[25] Mittler R. Oxidative stress, antioxidants and stress tolerance.Trends in Plant Science, 2002, 7(9): 405-410.
[26] Yin D M, Chen S M, Chen F D, et al . Morpho-anatomical and physiological responses of two Dendranthema species to waterlogging. Environmental and Experimental Botany, 2010, 68: 122-130.
[1] 刘婷. 紫花苜蓿在农业生态系统中的作用. 吉林畜牧兽医, 2005, (7): 21-23.
[2] 岳彦红, 齐晓, 王彦荣, 等. 35个10龄紫花苜蓿品种的持久性比较. 草业学报, 2014, 23(1): 58-64.
[3] 何忠曲, 郇恒福, 何华玄, 等.我国南方草业发展存在的问题与对策. 热带农业工程, 2009, 33(3): 83-86.
[4] 安渊, 陈凡毅, 王俊, 等.半秋眠和非秋眠紫花苜蓿品种耐涝性能研究. 中国草地, 2004, 26(4): 31-36.
[5] 周广生, 朱旭彤.湿害后小麦生理变化与品种耐湿性的关系. 中国农业科学, 2002, 35(7): 777-783.
[6] 李林, 邹冬生, 刘登望, 等.花生等农作物耐湿涝性研究进展.中国油料作物学报, 2004, 26(3): 105-110.
[7] 刘文革, 阎志红, 王川, 等.西瓜幼苗抗氧化系统对淹水胁迫的响应. 果树学报, 2006, 23(6): 860-864.
[8] 陈鹭真, 林鹏, 王文卿.红树植物淹水胁迫响应研究进展. 生态学报, 2006, (2): 586-593.
[13] 王学奎. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2006: 228-281.
[19] 刘晶, 才华, 刘莹, 等. 两种紫花苜蓿苗期耐盐生理特性的初步研究及其耐盐性比较. 草业学报, 2013, 22(2): 250-256.
[20] 李源, 刘贵波, 高洪文, 等.紫花苜蓿种质耐盐性综合评价及盐胁迫下的生理反应. 草业学报, 2010, 19(4): 79-86.
[21] 吴婧, 才华, 柏锡, 等. 转 GsGST 13 /SCMRP 基因双价苜蓿的耐盐性分析. 草业学报, 2014, 23(1): 257-265.
[22] 李真, 梅淑芳, 刘向蕾, 等.作物耐湿涝性研究进展. 湖北农业科学, 2009, 48(11): 2866-2868.

淹水对紫花苜蓿南北方品种抗氧化酶和无氧呼吸酶的影响

Effect of waterlogging on antioxidant and anaerobic respiratory enzymesin Medicago sativa varieties from southern and northern China

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