东祁连山高寒草地几种禾本科牧草根际促生菌研究

doi:10.11686/cyxb2016090

草业学报 ›› 2016, Vol. 25 ›› Issue (11): 173-177.DOI: 10.11686/cyxb2016090

东祁连山高寒草地几种禾本科牧草根际促生菌研究

李建宏^{1, 2}, 李雪萍^{1, 2}, 马文文^{1, 2}, 姚拓^{1, 2*, *}, 孙建军³, 蒋永梅^{1, 2}, 张建贵^{1, 2}, 师尚礼^{1, 2}

1.甘肃农业大学草业学院,甘肃兰州 730070;
2.草业生态系统教育部重点实验室,甘肃兰州 730070;
3.临洮县农业技术推广中心,甘肃临洮 730500

收稿日期:2016-03-04 出版日期:2016-11-20 发布日期:2016-11-20
通讯作者: *通信作者Corresponding author. E-mail:yaotuo@gsau.edu.cn
作者简介:李建宏(1986-),男,甘肃临潭人,在读博士。E-mail:lijianhong123@126.com
基金资助:
国家自然科学基金项目(31360584)和农业部国家牧草产业技术体系(CARS-35)项目资助

The study of plant growth promoting rhizobacteria (PGPR) from several Poaceous species in the East Chi-lien Mountains of China

LI Jian-Hong^{1, 2}, LI Xue-Ping^{1, 2}, MA Wen-Wen^{1, 2}, YAO Tuo^{1, 2, *}, SUN Jian-Jun³, JIANG Yong-Mei^{1, 2}, ZHANG Jian-Gui^{1, 2}, SHI Shang-Li^{1, 2}

1.College of Prataculture, Gansu Agricultural University, Lanzhou 730070, China;
2.Key Laboratory of Grassland Ecosystem, Ministry of Education, Lanzhou 730070, China;
3.Lintao County Agricultural Technology Promotion Center, Lintao 730500, China

Received:2016-03-04 Online:2016-11-20 Published:2016-11-20

摘要/Abstract

摘要： 本研究以东祁连山高寒草地常见的7种禾本科牧草为材料,通过分离培养的方法研究了其根际细菌的数量和分布,重点研究了其有固氮作用的细菌数量和分布。结果发现,7种植物根际均存在大量的细菌,总数在2.50×10⁶~17.07×10⁶ cfu/g,不同植物根际细菌的数量和分布不同,以高原早熟禾根际细菌数量最多,冰草和赖草根际细菌最少,其余禾草居中;根际固氮菌的数量和分布也因牧草种类不同而不同。7种供试植物根际分离到固氮菌201株。且细菌和PGPR菌株的数量均呈现“根系表面(RP)>根际土壤(RS)>根内(HP)”的分布趋势,表现出强烈的根际效应。

Abstract: This research studied the quantity and distribution of bacteria in the rhizosphere of seven Poaceous species from the east Chi-lien Mountains. The research employed a cultivation method, and mainly studied nitrogen fixing bacteria. It was found that there were large numbers of bacteria in rhizospheres of all 7 plant species studied. The total number of rhizosphere bacteria counted ranged from 2.5×10⁶ cfu/g to 17.07×10⁶ cfu/g, with Agropyron cristatum and Leymus secalinus having the lowest rhizosphere nitrogen-fixing bacterial populations and Poa alpigena having the highest, with the other 4 species intermediate. A total of 201 nitrogen-fixing strains were isolated from the 7 grass species studied. The quantity of bacteria and PGPR present a distribution trend: “root system surface (RP)>root surface soil (RS)>histoplan or interior of roots (HP)”.

李建宏, 李雪萍, 马文文, 姚拓, 孙建军, 蒋永梅, 张建贵, 师尚礼. 东祁连山高寒草地几种禾本科牧草根际促生菌研究[J]. 草业学报, 2016, 25(11): 173-177.

LI Jian-Hong, LI Xue-Ping, MA Wen-Wen, YAO Tuo, SUN Jian-Jun, JIANG Yong-Mei, ZHANG Jian-Gui, SHI Shang-Li. The study of plant growth promoting rhizobacteria (PGPR) from several Poaceous species in the East Chi-lien Mountains of China[J]. Acta Prataculturae Sinica, 2016, 25(11): 173-177.

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东祁连山高寒草地几种禾本科牧草根际促生菌研究

The study of plant growth promoting rhizobacteria (PGPR) from several Poaceous species in the East Chi-lien Mountains of China

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