一株抗马铃薯坏疽病莫海威芽孢杆菌(Bacillusmojavensis ZA1)培养条件的优化

doi:10.11686/cyxb2015142

草业学报 ›› 2016, Vol. 25 ›› Issue (2): 77-86.DOI: 10.11686/cyxb2015142

一株抗马铃薯坏疽病莫海威芽孢杆菌(Bacillusmojavensis ZA1)培养条件的优化

冯中红, 畅涛, 杨成德^*, 薛莉, 李旭, 杨小利

草业生态系统教育部重点实验室,甘肃农业大学草业学院,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

收稿日期:2015-03-17 出版日期:2016-02-20 发布日期:2016-02-20
通讯作者: E-mail:yangcd@gsau.edu.cn
作者简介:冯中红(1991-),女,甘肃临夏人,在读硕士。 E-mail:1142595813@qq.com
基金资助:
国家自然科学基金(No.31160122)资助

Optimization of culture conditions of a biocontrol agent Bacillus mojavensis ZA1 to antagonize Phoma foveata

FENG Zhong-Hong, CHANG Tao, YANG Cheng-De^*, XUE Li, LI Xu, YANG Xiao-Li

Key Laboratory of Grassland Ecosystem Gansu Agricultural University, Ministry of Education, College of Pratacultural Science, Gansu Agricultural University, Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S. Center for Grazingland Ecosystem Sustainability, Lanzhou 730070, China

Received:2015-03-17 Online:2016-02-20 Published:2016-02-20

摘要/Abstract

摘要： 为了提高生防菌株莫海威芽孢杆菌(Bacillus mojavensis ZA1)液体发酵的生物量,利用单因素试验设计与响应曲面试验设计相结合的方法对ZA1摇瓶发酵条件进行了优化。结果表明,ZA1的最佳培养基配比为氯化铵14.25 g、玉米粉19 g、马铃薯237 g、水1000 mL,最佳发酵条件为pH 7.7、培养温度28℃、转速180 r/min及发酵时间36 h,ZA1优化后活菌数为4.12×10¹⁰ CFU/mL。通过中心组合试验设计确定ZA1在10 L发酵罐中的最佳溶氧量为60%和转速为180 r/min;最优条件下,发酵ZA1的放罐时间确定为36 h,活菌数达到1.59×10¹¹ CFU/mL。该结果为利用ZA1开发生防制剂奠定了基础。

Abstract: This work aimed to identify optimum fermentation shaker-flask culture conditions for Bacillus mojavensis strain ZA1. Various single culture factors were combined according to a Box-Behnken design. The optimum ratio of ingredients for the culture medium for ZA1was found to be: 14.25 g ammonium chloride, 19 g corn flour, and 237 g potato, in 1000 mL distilled water. The optimum cultivation conditions were pH 7.7, temperature 28℃, and shaken at 180 r/min for 36 h. Under these conditions, the viable count of B. mojavensis strain ZA1 was 4.12×10¹⁰ colony-forming units per mL (CFU/mL). Further testing using the central composite design method in a 10 L fermentation tank found the optimum for dissolved oxygen level and shaker rotation speed to be 60% saturation and 180 r/min respectively. Under these conditions, when fermentation time was 36 h in the fermentation tank, the viable count of B. mojavensis strain ZA1 reached 1.59×10¹¹ CFU/mL. The results of this study lay a solid foundation for the development of methodology for large scale production of biocontrol agents.

冯中红, 畅涛, 杨成德, 薛莉, 李旭, 杨小利. 一株抗马铃薯坏疽病莫海威芽孢杆菌(Bacillusmojavensis ZA1)培养条件的优化[J]. 草业学报, 2016, 25(2): 77-86.

FENG Zhong-Hong, CHANG Tao, YANG Cheng-De, XUE Li, LI Xu, YANG Xiao-Li. Optimization of culture conditions of a biocontrol agent Bacillus mojavensis ZA1 to antagonize Phoma foveata[J]. Acta Prataculturae Sinica, 2016, 25(2): 77-86.

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一株抗马铃薯坏疽病莫海威芽孢杆菌(Bacillusmojavensis ZA1)培养条件的优化

Optimization of culture conditions of a biocontrol agent Bacillus mojavensis ZA1 to antagonize Phoma foveata

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