纤维素降解细菌的筛选,生物学特性及降解效果

doi:10.11686/cyxb2014172

草业学报 ›› 2016, Vol. 25 ›› Issue (3): 60-66.DOI: 10.11686/cyxb2014172

纤维素降解细菌的筛选,生物学特性及降解效果

贾辉¹, 陈秀蓉^{1, *}, 芦光新^{2, *}, 孔雅丽¹, 杨成德¹

1.甘肃农业大学草业学院,草业生态系统重点实验室,甘肃兰州 730070;
2.青海大学农牧学院草业科学系,青海西宁 810016

收稿日期:2014-04-08 出版日期:2016-03-20 发布日期:2016-03-20
通讯作者: E-mail:chenxiurong@gsau.edu.cn,lugx74@qq.com
作者简介:贾辉(1981-),男,甘肃古浪人,在读博士.E-mail:jiah66@163.com
基金资助:
国家自然科学基金项目(31460152,41261064)和青海省科技厅项目(2014-ZJ-924Q)资助

Isolation of cellulose-degrading bacteria and determination of their degradation activity

JIA Hui¹, CHEN Xiu-Rong^{1, *}, LU Guang-Xin^{2, *}, KONG Ya-Li¹, YANG Cheng-De¹

1.Key Lab of Grassland Ecosystem of MOE, College of Prataculture, Gansu Agricultural University, Lanzhou 730070, China;
2.Department of Grassland Science,Agriculture and Animal Husbandry College of Qinghai University, Xining 810016, China

Received:2014-04-08 Online:2016-03-20 Published:2016-03-20

摘要/Abstract

摘要： 为了从东祁连山高寒草甸土壤中分离筛选纤维素分解细菌,本研究根据在羧甲基纤维素钠培养基和滤纸平板培养基上的生长情况,初步筛选出3株具有较强纤维素分解能力的细菌,并对其生长条件进行了初步研究,结果表明,3株菌的最适生长温度范围为25~30℃;最适生长pH因菌种不同位于5~8之间;最适生长盐浓度位于4%~5%.菌株X1-2具有较好降解特性,根据形态观察,革兰氏染色及16S rRNA系统发育比较,鉴定该菌为芽孢杆菌(Bacillus sp.),是一株十分具有开发生产纤维素酶能力的菌株.

Abstract: Eight bacterial strains were isolated from soil in the east Qilian Mountains. Of these, four strains had a higher capacity for degradation of plant cellulose when cultured on a CMC-Na plate medium. The growth characteristics of the four strains were studied, and the optimum temperature range of the 4 active strains was 25-30℃, the optimum pH for growth was 5-8, and the optimum sodium concentration was 4% to 5%. A strain designated X1-2 possessed the highest cellulose degradation ability. Based on morphology, gram stain reaction, and 16S rRNA phylogenetic comparison, this strain was identified as a member of the genus Bacillus (Bacillus sp.). It is a bacterium with strong plant cellulose degradation ability.

贾辉, 陈秀蓉, 芦光新, 孔雅丽, 杨成德. 纤维素降解细菌的筛选,生物学特性及降解效果[J]. 草业学报, 2016, 25(3): 60-66.

JIA Hui, CHEN Xiu-Rong, LU Guang-Xin, KONG Ya-Li, YANG Cheng-De. Isolation of cellulose-degrading bacteria and determination of their degradation activity[J]. Acta Prataculturae Sinica, 2016, 25(3): 60-66.

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纤维素降解细菌的筛选,生物学特性及降解效果

Isolation of cellulose-degrading bacteria and determination of their degradation activity

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