丛枝菌根真菌与植物共生修复陕北石油污染土

doi:10.11686/cyxb2015446

草业学报 ›› 2016, Vol. 25 ›› Issue (7): 87-94.DOI: 10.11686/cyxb2015446

丛枝菌根真菌与植物共生修复陕北石油污染土

山宝琴^*, 屈萌萌, 李皎, 李超

延安大学石油工程与环境工程学院,陕西延安 716000

收稿日期:2015-09-23 修回日期:2015-12-10 出版日期:2016-07-20 发布日期:2016-07-20
作者简介:山宝琴(1970-),女,新疆乌鲁木齐人,副教授,博士。E-mail:xiaoshanbao@163.com
基金资助:
陕西省高水平大学专项资金项目(No.2013SXTS03),延安市科技项目(No.2014KS-03),延安大学引导项目(No.YD2015-14)和国家大学生创新项目(No.201410719009)资助

Bioremediation of petroleum contaminated soil by plants and arbuscular mycorrhizal fungi in Northern Shaanx

SHAN Bao-Qin^*, QU Meng-Meng, LI Jiao, LI Chao

School of Petroleum Engineering and Environmental Engineering, Yan’an University, Yan’an 716000, China

Received:2015-09-23 Revised:2015-12-10 Online:2016-07-20 Published:2016-07-20

摘要/Abstract

摘要： 研究先期筛选了4种陕北乡土植物黄花蒿、艾蒿、柠条和沙打旺,及植物根围匹配的丛枝菌根(arbuscular mycorrhiza, AM)真菌菌种,通过盆栽试验测定AM真菌孢子密度、宿主植物根系菌丝侵染率、植物株高、干重和土壤总石油烃的降解率。旨在研究接种AM真菌对污染土壤中总石油烃的降解率以及对宿主植物生长的影响, 验证菌根修复效果并筛选最佳宿主植物和菌种组合。结果表明,1) AM真菌孢子密度最大值出现在石油浓度0~5000 mg/kg土壤,并随污染浓度加大而显著减少,40000 mg/kg 时达最小值。植物菌根侵染率在土壤被污染的情况下显著高于无污染对照,同时接种AM后孢子密度及菌根侵染率均显著高于未接种;2) 植物株高和干重都随石油污染浓度加大而显著降低,接种处理后植物株高平均增加16.77%,植物鲜重平均增加了22.56%; 3) 植物接种AM真菌后对石油烃降解率明显提高,比不接种平均增加15.35%。其中,柠条-地球囊霉组合的石油烃降解率最高(平均为73.81%);4) 相关分析表明,接种AM真菌后石油烃降解率与石油烃浓度呈极显著负相关,与植物株高、干重和真菌孢子密度呈显著正相关。

Abstract: A new bioremediation technique utilizing the symbiotic function between host plant and arbuscular mycorrhizal fungi (AMF) for petroleum contaminated soil was investigated in Northern Shaanxi in 2015. Four species of native plants in Northern Shaanxi studied were Artemisia annu, Artemisia argyi, Caragana korshinskii and Astragalus adsurgens, and AMF species were isolated from the rhizosphere of each species and used to inoculate field grown plants in contaminated soil to test effects of AMF on the degradation of petroleum hydrocarbon in soil and to select the best plant-AMF match. It was found that AMF spore densities were highest in soil with 0-5000 mg petroleum/kg soil, and significantly decreased with increasing petroleum concentration. AMF hyphal densities were higher in petroleum contaminated soil than in uncontaminated soil. The inoculation with AMF increased spore density and the percentage of AM colonization. Plant height and dry weight were decreased with increasing petroleum concentration, but both were significantly promoted by AMF inoculation AMF, with plant height increased by 16.77% and dry weight by 22.56%. Degradation efficiency of petroleum hydrocarbon in soil was enhanced by AMF inoculation, and was 15.35% higher than that of uninoculated soil. Among the plant-AMF combinations, Caragana korshinskii and Glomus geosporum was the best for petroleum degradation, and the degradation rate was 73.81% after 2 months. After inoculation with the selected AMF species, degradation rates of petroleum hydrocarbon and petroleum concentration in soil showed an obvious negative correlation, but degradation rates were significantly positively correlated with spore density, plant height and plant dry weight.

山宝琴, 屈萌萌, 李皎, 李超. 丛枝菌根真菌与植物共生修复陕北石油污染土[J]. 草业学报, 2016, 25(7): 87-94.

SHAN Bao-Qin, QU Meng-Meng, LI Jiao, LI Chao. Bioremediation of petroleum contaminated soil by plants and arbuscular mycorrhizal fungi in Northern Shaanx[J]. Acta Prataculturae Sinica, 2016, 25(7): 87-94.

参考文献

[1] Li Q, Huang T L, Shang X Q, et al . Distribution and sources of polycyclic aromatic hydrocarbon (PAils) pollutants in soils of Yanhe River basin, north of Shaanxi province. Journal of Shaanxi Normal University, 2011, 39(2): 76-80.
[2] Volante A, Lingua G, Cesaro P, et al . Influence of three species of arbuscular mycorrhizal fungi on the persistence of aromatic hydrocarbons in contaminated substrates. Mycorrhiza, 2005, 16: 43-50.
[3] Chen L, Zhang F W, Liu S Y, et al . Experimental study on in-situ contaminated soil in bioremediation technique for oil Zhongyuan Oil Field. Chinese Journal of Environmental Engineering, 2011, 5(10): 2385-2389.
[4] Wu Q S, Yuan F Y, Fei Y J, et al . Effects of arbuscular mycorrhizal fungi on aggregate stability, GRSP, and carbohydrates of white clover.Acta Prataculturae Sinica, 2014, 23(4): 269-275.
[5] Liu R J, Chen Y L. Mycorrhizology[M]. Beijing: Science Press, 2007.
[6] Smith M R, Charvat I, Jacobson R L. Arbuscular mycorrhizae promote establishment of prairie species in a tallgrass prairie restoration. Canadian Journal of Botany, 1998, 76: 1947-1954.
[7] Noyd R K, Pflegar F L, Norland M R. Field response to added organic matter, arbuscular mycorrhizal fungi and fertilizer in reclamation of taconite iron ore tailing. Plant and Soil, 1996, 179: 89-97.
[8] Luo Q Y, Wang X J, Lin S S, et al . Mechanism and application of bioremediation to heavy metal polluted soil using arbuscular mycorrhizal fungi. Acta Ecologica Sinica, 2013, 33(13): 3898-3906.
[9] Bi Y L, Wu F Y, Wu Y K. Application of arbuscular mycorrhizas in ecological restoration of areas affected by coal mining. Acta Ecologica Sinica, 2005, 25(8): 2068-2073.
[10] Geng C N, Li P J, Chen S H, et al . Effects of different arbuscular mycorrhizal fungi on oil tolerance of Trifolium subterraneum L. Chinese Journal of Applied & Environmental Biology, 2002, 8(6): 648-652.
[11] Geng C N, Li P J, Chen S H, et al . Effects of different arbuscular mycorrhizal fungi on Tagetes erecta growth and diesel degradation. Chinese Journal of Applied Ecology, 2003, 14(10): 1775-1779.
[12] Wang L P, Guo G X, Hua S L, et al . Bioremediation of petroleum-contaminated soil with arbuscular mycorrhizal fungi and plant. Journal of China University of Mining & Technology, 2009, 38(1): 90-95
[13] He X, Wei W, Wu H. Microbe and mycorrhizal bioremediation of oil-contaminated soil. Soil, 2004, 36(6): 675-677.
[14] Fang F, He X L. Ecological research on arbuscular mycorrhizal fungi from the rhizospere of Caragana korshinskii in Northwest arid region. Agricultural Research in the Arid Areas, 2007, 25(2): 201-206.
[15] Han H L, Tang J, Jiang H, et al . Synergy between fungi and bacteria in fungi-bacteria augmented remediation of petroleum-contaminated soil. Environment Science, 2008, 29(1): 189-196.
[16] Ianson D C, Allen M F. The effects of soil texture on extraction of vesicular arbuscular mycorrhizal spores from arid soils. Mycologia, 1986, 78: 164-168.
[17] Phillips J M, Hayman D S. Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society, 1970, 55: 158-161.
[18] Shan B Q, Zhang Y T, Cao Q L, et al . Content and distribution characteristics of heavy metals in different soil layers of petroleum polluted soil in Northern Shaanxi. Environment Pollution and Control, 2014, 36(3): 20-25.
[19] Zhang Y F, Bi Q, Yang Y F, et al . Arbuscular mycorrhizal fungi diversity in saline-alkaline Leymus chinensis grasslands on the Songnen Plain. Acta Prataculturae Sinica, 2015, 24(9): 80-88.
[20] Shan B Q, Zhang Y T, Cao Q L, et al . Growth responses of six Leguminous plants adaptable in Northern Shaanxi to petroleum contaminated soil. Environment Science, 2014, 35(3): 1125-1130.
[21] Chen Y L, Chen B D, Liu L, et al . The role of arbuscular mycorrhizal fungi in soil nitrogen cycling. Acta Ecologica Sinica, 2014, 34(17): 4807-4815.
[22] Jussi H, Kirsten S J, Kielo H, et al . Effect of pinus syluestris root growth and mycorrhizosphere development on bacterial carbon source utilization and hydrocarbon oxidation in forest and petroleum-contaminated soils. Canadian Journal of Microbiology, 1998, 46: 451-464.
[23] Xiao M, Gao Y Z, Ling W T, et al . Effects of arbuscular mycorrhizal fungi on enzymes activity in soils contaminated by phenanthrene and pyrene. China Environmental Science, 2009, 29(6): 668-672.
[24] Siciliano S D, Germida J J, Banks K, et al . Changes in microbial community composition and function during a polyaromatic hydrocarbon phytoremediation field trial. Applied and Environmental Microbiology, 2003, 69: 483-489.
[1] 李琦, 黄廷林, 尚晓青, 等. 陕北延河流域土壤多环芳烃分布特征及来源. 陕西师范大学学报, 2011, 39(2): 76-80.
[3] 陈立, 张发旺, 刘少玉, 等. 中原油田石油污染土壤原位生物修复技术实验研究. 环境工程学报, 2011, 5(10): 2385-2389.
[4] 吴强盛, 袁芳英, 费永俊, 等. 菌根真菌对白三叶根际团聚体稳定性、球囊霉素相关土壤蛋白和糖类物质的影响. 草业学报, 2014, 23(4): 269-275.
[5] 刘润进, 陈应龙. 菌根学[M]. 北京: 科学出版社, 2007.
[8] 罗巧玉, 王晓娟, 李双双, 等. AM真菌对重金属污染土壤生物修复的应用与机理. 生态学报, 2013, 33(13): 3898-3906.
[9] 毕银丽, 吴福勇, 武玉坤. 丛枝菌根在煤矿区生态重建中的应用. 生态学报, 2005, 25(8): 2068-2073.
[10] 耿春女, 李培军, 陈素华, 等. 不同AM真菌对三叶草耐油性的影响. 应用与环境生物学报, 2002, 8(6): 648-652.
[11] 耿春女, 李培军, 陈素华, 等. 不同丛枝菌根真菌对万寿菊生长及柴油降解率的影响. 应用生态学报, 2003, 14(10): 1775-1779.
[12] 王丽萍, 郭光霞, 华素兰, 等. 菌根真菌-植物对石油污染土壤修复实验研究. 中国矿业大学学报, 2009, 38(1): 90-95.
[13] 何翊, 魏薇, 吴海. 菌剂-菌根联合修复石油污染土壤的实验研究. 土壤, 2004, 36(6): 675-677.
[14] 方菲, 贺学礼. 旱生条件下柠条锦鸡儿AM真菌生态学研究. 干旱地区农业研究, 2007, 25(2): 201-206.
[15] 韩慧龙, 汤晶, 江皓, 等. 真菌-细菌修复石油污染土壤的协同作用机制研究. 环境科学, 2008, 29(1): 189-196.
[18] 山宝琴, 张永涛, 曹巧玲, 等. 陕北原油污染土壤重金属含量及分布特性分析. 环境污染与防治, 2014, 36(3): 20-25.
[19] 张义飞, 毕琪, 杨允菲, 等. 松嫩平原盐碱化羊草群落中AM真菌物种资源及侵染率研究. 草业学报, 2015,24(9): 80-88.
[20] 山宝琴, 张永涛, 曹巧玲, 等. 6种陕北适生豆科植物生长对原油污染土壤的响应. 环境科学, 2014, 35(3): 1125-1130.
[21] 陈永亮, 陈保冬, 刘蕾, 等. 丛枝菌根真菌在土壤氮素循环中的作用. 生态学报, 2014, 34(17): 4807-4815.
[23] 肖敏, 高彦征, 凌婉婷, 等. 菲、芘污染土壤中丛枝菌根真菌对土壤酶活性的影响. 中国环境科学, 2009, 29(6): 668-672.

丛枝菌根真菌与植物共生修复陕北石油污染土

Bioremediation of petroleum contaminated soil by plants and arbuscular mycorrhizal fungi in Northern Shaanx

RichHTML

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics