Abstract: To identify heredity differences among 17 elite hybrid lines selected from three cross combinations of ‘1867’בLongshu.7’, ‘MB09’בLongshu.7’ and ‘MB09’בLongshu.6’ potatoes, we used four parent lines as controls and analyzed genetic differences using AFLP molecular markers. A total of 321 loci were amplified using 10 AFLP primer pairs, of which 277 were polymorphic, accounting for 87.29% of all loci. The experiment established an AFLP fingerprint map of elite hybrid lines. The average polymorphism information content (PIC) from 21 samples was 0.5617; Nei’s gene diversity index was 0.3623 and the Shannon information index was 0.5407. The average genetic distance (GD) of the tested lines was 0.5281 and were classified into four categories based on a GD value of 0.51: ‘1867’, A-10, A-12, A-21 and A-29; ‘Longshu.6’, ‘Longshu.7’, A-14, A-23, A-26, B-13 and B-14; ‘MB09’, B-1, B-2, B-7, B-15, B-20 and C-22, and C-2 and C-21. This study provided a basis for selection and utilization of potato hybrid parents and breeding of new hybrid varieties.