日粮NDF水平对断奶獭兔血液免疫细胞与肠道特性的影响

doi:10.11686/cyxb2015151

草业学报 ›› 2016, Vol. 25 ›› Issue (9): 197-203.DOI: 10.11686/cyxb2015151

• 研究简报 • 上一篇

日粮NDF水平对断奶獭兔血液免疫细胞与肠道特性的影响

任战军^1,2, 高玉琪², 王金丽², 李发弟^1*

1.甘肃农业大学动物科技学院,甘肃兰州 730070;
2.西北农林科技大学动物科技学院,陕西杨凌 712100

收稿日期:2015-03-17 出版日期:2016-09-20 发布日期:2016-09-20
通讯作者: E-mail: lifadi@gsau.edu.cn
作者简介:任战军(1966-),男,陕西淳化人,副教授。E-mail: renzhanjun@nwsuaf.edu.cn
基金资助:
陕西省农业公关项目(2010K01-16)和西安市农业攻关项目(NC09049-4)资助

The effect of dietary NDF levels on blood immune system cells and intestinal characteristics of Rex rabbits at weaning

REN Zhan-Jun^1,2, GAO Yu-Qi², WANG Jin-Li², LI Fa-Di^1,*

1.College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2.College of Animal Science and Technology, Northwest Agriculture & Forestry University, Yangling 712100, China

Received:2015-03-17 Online:2016-09-20 Published:2016-09-20

摘要/Abstract

摘要： 研究日粮中性洗涤纤维(NDF)水平对断奶獭兔血液免疫细胞、小肠分泌型免疫球蛋白A(sIgA)、盲肠微生物及盲肠pH的影响,为揭示日粮NDF和肠道健康关系和断奶獭兔日粮的配制提供理论依据。选择35日龄体重差异不显著的断奶獭兔56只,随机分成4组,每组14只(每只为一个重复)。分别饲喂NDF水平为29%,33%,37%,41%的日粮。预试7 d,正式试验28 d。试验结束后各组随机选取6只屠宰采样,测量血液及肠道各项指标。结果表明,随着日粮NDF水平提高,断奶獭兔白细胞数无显著差异 (P>0.05);血液淋巴细胞数上升(P<0.05);十二指肠和空肠sIgA+细胞数显著增加(P<0.05);盲肠总细菌数无显著差异(P>0.05);拟杆菌数先增加后减少,33%组显著高于其他各组(P<0.05);乳酸菌和大肠杆菌数降低,且乳酸菌29%组显著高于37%组和41%组(P<0.05),大肠杆菌41%组显著低于29%和33%组(P<0.05);盲肠pH值升高;腹泻率和死亡率降低。日粮NDF 水平提高对断奶獭兔的肠道健康有利,NDF 41%组断奶獭兔肠道最健康。

Abstract: This research evaluated the effect of dietary neutral detergent fiber (NDF) level on blood immune cells and intestinal characteristics of Rex rabbits at weaning in order to define the relationship between dietary NDF and intestinal health, and provide a theoretical basis for diet formulation for Rex rabbits at weaning. Fifty-six newly weaned rabbits (35 d) were randomly assigned into 4 groups (14 rabbits per group with each rabbit considered a replicate for statistical purposes), and the groups received different diets with NDF levels of 29%, 33%, 37% and 41%, respectively. The experiment comprised a 5 d pre-trial period and a 28 d experiment period. Diarrhea rate and mortality were recorded during the entire trial. At the end of the trial, 6 rabbits from each group were slaughtered for sampling of blood and intestinal indicators. It was found that with increasing dietary NDF, the count of white blood cells did not differ between treatments (P>0.05), while the lymphocyte count was significantly increased (P<0.05). sIgA+ cell counts in the duodenum and jejunum were increased with dietary NDF level (P<0.05), but this trend was not observed in the ileum (P>0.05). Moreover, the total number of intestinal bacteria was similar for the 4 treatments (P>0.05). Bacteroides was increased at lower MDF with the 33% NDF group being significantly higher than the other groups (P<0.05). Cecum Lactobacillus and Escherichia coli numbers generally decreased with increasing dietary NDF level: specifically, the 29% NDF group got the highest Lactobacillus count while 41% NDF group had the lowest E. coli number (P<0.05). Furthermore, cecum pH was increased with dietary NDF level while diarrhea rate and mortality were decreased. In summary, among tested treatments, the 41% dietary NDF level optimized the intestinal health of Rex rabbits at weaning.

任战军, 高玉琪, 王金丽, 李发弟. 日粮NDF水平对断奶獭兔血液免疫细胞与肠道特性的影响[J]. 草业学报, 2016, 25(9): 197-203.

REN Zhan-Jun, GAO Yu-Qi, WANG Jin-Li, LI Fa-Di. The effect of dietary NDF levels on blood immune system cells and intestinal characteristics of Rex rabbits at weaning[J]. Acta Prataculturae Sinica, 2016, 25(9): 197-203.

参考文献

[1] Peng Q H. Effect of Different Levels of ADF Diets on Growth Performance, Intestinal Mucosal Immunity and Caecum Fermentation of Weaning Rabbits[D]. Ya’an: Sichuan Agricultural University, 2008.
[2] Laissue J, Gebbers J. The intestinal barrier and the gut-associated lymphoid tissue. Current Studies in Hematology & Blood Transfusion, 1991, (59): 19-43.
[3] Tao Z Y, Li F C. Effect of dietary NDF levels on production performance, digestibility, metabolism and caecunm fermentation of weaning to two month old meat rabbits. Acta Zoonutrimenta Sinica, 2005, 17(4): 56-61.
[4] Chao H Y, Jing W Q, Xia C F, et al . Effects of dietary NDF levels on reproductive performance, serum biochemical indices and hormone levels of gestating rabbits. Journal of China Agricultural University, 2015, 20(6): 181-188.
[5] Gu Z L, Huo G C, Zhang Y H, et al . The diet crude fiber and physiological function of rabbit. Feed Industry, 2002, 23(11): 16-18.
[6] Gu Z L, Li J, Zhang Y H, et al . Effect of crude fiber level on diarrhea, intestinal pH and caecal microbe of rex rabbit. Chinese Agricultural Science Bulletin, 2008, 24(2): 21-25.
[7] Carlos, Julian Wiseman. Nutrition of the Rabbit[M]. Tang L M, translation. Beijing: China Agriculture Press, 2015: 7.
[8] Wang J L, Ren Z J, Song H X, et al . Effect of diets NDF levels on growth performance and intestinal mucosa morphology of weaned rex rabbit. Journal of Northwest A-F University: Natural Science Edition, 2012, 40(8): 34-38.
[9] Kaufhold J, Hammon H, Blum J. Fructo-oligosaccharide supplementation: effects on metabolic, endocrine and hematological traits in veal calves. Journal of Veterinary Medicine Series A, 2000, 47(1): 17-29.
[10] Kagnoff M. Immunology of the intestinal tract. Gastroenterology, 1993, 105(5): 1275.
[11] Wang S Q, Li C, Li F D, et al . Effects of neutral detergent fiber level of starter on growth performance and digestive tract development of suckling lambs. Chinese Journal of Animal Nutrition, 2014, 26(8): 2169-2175.
[12] Manhart N, Spittler A, Bergmeister H, et al . Influence of fructooligosaccharides on Peyer’s patch lymphocyte numbers in healthy and endotoxemic mice. Nutrition, 2003, 19(7/8): 657-660.
[13] Schley P D, Field C J. The immune-enhancing effects of dietary fibres and prebiotics. British Journal of Nutrition, 2002, 87: 221-230.
[14] Shen X F. Histology and Embryology of Domestic Animal[M]. Beijing: China Agriculture Press, 2001.
[15] Gouet P, Fonty G. Evolution de la microflore digestive du lapin holoxénique de la naissance au sevrage. Annales De Biologie Animale Biochimie Biophysique, 1973, 13: 733-735.
[16] Chadwick V, Anderson R. Microorganisms and Their Products in Inflammatory Bowel Disease[M]. New York: Elsevier, 1992: 241-258.
[17] Aries V, Hill M. Degradation of steroids by intestinal bacteria I. Deconjugation of bile salts. Biochimica et Biophysica Acta (BBA)-Lipids and Lipid Metabolism, 1970, 202(3): 526-534.
[18] Hill M J. Role of Gut Bacteria in Human Toxicology and Pharmacology[M]. London: Taylor & Francis, 1995.
[19] Brownlee I A. The physiological roles of dietary fibre. Food Hydrocolloids, 2011, 25(2): 238-250.
[20] García J, Gidenne T, Falcao-e-Cunha L, et al . Identification of the main factors that influence caecal fermentation traits in growing rabbits. Animal Research, 2002, 51(2): 165-173.
[21] Gomez-Conde M S, Garcia J, Chamorro S, et al . Neutral detergent-soluble fiber improves gut barrier function in twenty-five-day-old weaned rabbits. Journal of Animal Science, 2007, 85(12): 3313-3321.
[22] Zhang L T, Diao Q Y, Li Y L, et al . Recent advances in nutrition mechanism and requirement of neutral detergent fiber. China Plant-eating Animal Science, 2013, 33(1): 57-61.
[23] Zhu Y L. The Effect of Dietary Piber. Starch on Growing Rabbits’ Growth, Immune Response, Intestinal Bacterial Community Composition and Intestinal Proteomics Study[D]. Jinan: Shandong Agricultural University, 2013.
[24] Zhan J S, Zhan K, Liu M M, et al . Effects of alfalfa pellet feed on slaughter performance, organ weights and blood biochemical indices of gees. Acta Prataculturae Sinica, 2015, 24(8): 181-187.
[1] 彭全辉. 不同 ADF水平饲粮对肉兔生产性能, 肠道粘膜免疫及盲肠发酵的影响[D]. 雅安: 四川农业大学, 2008.
[3] 陶志勇, 李福昌. 日粮中性洗涤纤维水平对断奶至 2 月龄肉兔生产性能, 消化代谢和盲肠发酵的影响. 动物营养学报, 2005, 17(4): 56-61.
[4] 晁洪雨, 井文倩, 夏春峰, 等. 日粮NDF水平对妊娠母兔繁殖性能、血清生化指标及激素的影响. 中国农业大学学报, 2015, 20(6): 181-188.
[5] 谷子林, 霍贵成, 张玉华, 等. 日粮粗纤维及对家兔的生理功能. 饲料工业, 2002, 23(11): 16-18.
[6] 谷子林, 李江, 张玉华, 等. 日粮粗纤维水平对断乳力克斯兔腹泻率、消化道pH和盲肠菌群的影响. 中国农学通报, 2008, 24(2): 21-25.
[7] Carlos, Julian Wiseman. 家兔营养[M]. 唐良美, 译. 北京: 中国农业出版社, 2015: 7.
[8] 王金利, 任战军, 宋洪新, 等. 日粮 NDF 水平对断奶獭兔生长性能和肠道形态的影响. 西北农林科技大学学报: 自然科学版, 2012, 40(8): 34-38.
[11] 王世琴, 李冲, 李发弟, 等. 开食料中性洗涤纤维水平对哺乳羔羊生长性能和消化道发育的影响. 动物营养学报, 2014, 26(8): 2169-2175.
[14] 沈霞芬. 家畜组织学与胚胎学[M]. 北京: 中国农业出版社, 2001.
[22] 张立涛, 刁其玉, 李艳玲, 等. 中性洗涤纤维生理营养与需要量的研究进展.中国草食动物, 2013, 33(1): 57-61.
[23] 朱岩丽. 日粮纤维/淀粉对生长肉兔生长发育、免疫应答、肠道菌群构成的影响及肠道蛋白质组学初探[D]. 济南: 山东农业大学, 2013.
[24] 占今舜, 詹康, 刘明美, 等. 苜蓿草颗粒饲料对鹅屠宰性能、器官和血液生化指标的影响. 草业学报, 2015, 24(8): 181-187.

日粮NDF水平对断奶獭兔血液免疫细胞与肠道特性的影响

The effect of dietary NDF levels on blood immune system cells and intestinal characteristics of Rex rabbits at weaning

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics