镉污染和接种丛枝菌根真菌对紫花苜蓿生长和氮吸收的影响

doi:10.11686/cyxb2016133

摘要/Abstract

摘要： 采用盆栽试验研究在3个镉污染(0,6和12 mg Cd/kg)水平下,接种5种丛枝菌根真菌[分别接种聚丛球囊 Glomus aggregatum(Ga)、幼套球囊霉 G. etunicatum (Ge)、扭形球囊霉 G. tortuosum(Gt)、根内球囊霉 G. intraradices(Gi)和地表球囊霉 G. versiforme(Gv),以不接种为对照]对紫花苜蓿生长和氮吸收的影响。结果表明:与不加镉(0 mg Cd/kg)处理相比,接种Ga、Gi 和Gt菌种处理的紫花苜蓿菌根侵染率在12 mg Cd/kg条件下降低了33.90%、19.17%和31.95%;0 mg Cd/kg水平下接种Gt菌种紫花苜蓿总生物量分别比接种 Ga、Ge、Gi和Gv菌种处理显著高出33.19%、67.74%、57.29%和34.91%,但在12 mg Cd/kg水平时总生物量与以上菌种处理相比,分别降低16.67%、34.07%、32.96%和52.76%;在镉浓度为12 mg Cd/kg时,接种Gv菌种处理的紫花苜蓿株高、根瘤菌数量、地上生物量、总生物量、地上植株氮含量和整株含氮量与不接种处理相比,分别增加 65.41%、95.24%、61.87%、50.30%、5.83%和71.55%;随镉浓度增加接种Gv菌种处理紫花苜蓿土壤中铵态氮(NH₄⁺-N)和硝态氮(N₃^--N)浓度显著下降。综上分析,在镉污染条件下,接种Gv菌种能促进紫花苜蓿生长和氮吸收;当土壤镉浓度超过6 mg Cd/kg时,接种Gt菌种不利于紫花苜蓿的生长。

Abstract: A pot experiment in a greenhouse was conducted to investigate the growth and nitrogen (N) uptake of Medicago sativa inoculated with different arbuscular mycorrhizal fungi [Glomus aggregatum (Ga), Glomus etunicatum (Ge), Glomus intraradices (Gi), Glomus tortuosum (Gt), Glomus versiforme (Gv)] or un-inoculated in soil without or with cadmium at two concentrations (6 and 12 mg Cd/kg). Mycorrhizal colonization of alfalfa inoculated with Ga, Gi, and Gt was 33.90%, 19.17%, and 31.95% lower, respectively, in 12 mg Cd/kg soil than in 0 mg Cd/kg soil. In soil without Cd, the biomass of the Gt-inoculated plants was 33.19%, 64.74%, 57.29%, and 34.91% higher than that in the Ga, Ge, Gi, and Gv treatments, respectively. In 12 mg Cd/kg soil, the biomass of the Gt-inoculated plants was 16.67%, 34.07%, 32.96%, and 52.76% lower than that of those inocluated with Ga, Ge, Gi, and Gv, respectively. Compared with uninoculated plants, those inoculated with Gv showed significantly increased shoot height, number of nodules, shoot biomass, total biomass, shoot N content, and total N content (by 65.41%, 95.24%, 61.87%, 50.30%, 5.83%, and 71.55%, respectively) in soil containing 12 mg Cd/kg. The N₄⁺-N and N₃^--N concentrations in soil significantly decreased with increasing Cd levels in the Gv-inoculation treatments. These results showed that Gv inoculation promoted the growth and N uptake of alfalfa in Cd-contaminated soils, whereas Gt inoculation could not benefit the growth of alfalfa grown in soils containing more than 6 mg Cd/kg.

刘芳, 景戍旋, 胡健, 肖燕, 张英俊. 镉污染和接种丛枝菌根真菌对紫花苜蓿生长和氮吸收的影响[J]. 草业学报, 2017, 26(2): 69-77.

LIU Fang, JING Shu-Xuan, HU Jian, XIAO Yan, ZHANG Ying-Jun. Effects of cadmium and arbuscular mycorrhizal fungi inoculation on the growth and nitrogen uptake of alfalfa (Medicago sativa)[J]. Acta Prataculturae Sinica, 2017, 26(2): 69-77.

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