硫素形态对苗期菘蓝生长生理特性及次生代谢的影响

doi:10.11686/cyxb2018223

摘要/Abstract

摘要： 为探讨不同硫素形态对菘蓝生长、生理及次生代谢的影响,设置CK(无硫)、Na₂SO₄、Na₂SO₃、NaHSO₃、Na₂S和Na₂S₂O₃ 6个试验处理,通过盆栽试验研究不同硫素形态对菘蓝生长、光合生理、氮硫代谢、营养物质和靛蓝、靛玉红含量的影响。结果表明,Na₂SO₄处理最有利于菘蓝叶片鲜重和干重的积累,NaHSO₃处理次之,与CK相比其他处理组对其生长和生物量积累无显著促进作用。硫素处理后叶片的光合色素含量及光合效率均显著高于无硫处理,其中以NaHSO₃处理后净光合速率最高,Na₂S处理叶绿素含量最高。不同形态硫素处理对氮硫代谢的影响显著不同,其中以Na₂SO₃处理对氮代谢酶活性促进作用最显著,Na₂SO₄处理可显著提高硫代谢酶活性,而缺硫(CK)对氮硫代谢酶活性无显著的抑制作用。叶片中营养物质含量对不同硫素的响应存在差异,其中缺硫显著降低叶片中可溶性蛋白和游离氨基酸含量,可溶性糖和可溶性蛋白含量以NaHSO₃处理下最高,而游离氨基酸含量以Na₂SO₄处理最高。不同于生理响应,次生代谢物质靛蓝和靛玉红含量以Na₂S处理最高。综合分析不同硫素形态对菘蓝叶采收品质的影响,以Na₂SO₄处理菘蓝后其生长状态处于最佳水平,且叶内活性成分也相对较高。可为菘蓝栽培选用适宜的硫素形态提供理论参考。

关键词: 硫素形态, 菘蓝, 光合生理, 氮硫代谢, 营养物质, 次生代谢物质

Abstract: To investigate the effect of S in different chemical forms on the growth, physiology and secondary metabolism of Isatis indigotica, an experiment with six treatments [CK (no added S), Na₂SO₄, Na₂SO₃, NaHSO₃, Na₂S and Na₂S₂O₃] was set up, and the changes in leaf fresh and dry weight, photosynthetic physiology, nitrogen and sulfur metabolism, nutritional components and content of indigo and indirubin studied, using a pot trial. It was found that the fresh and dry weight were greatest in the Na₂SO₄ treatment, with the NaHSO₃ treatment being second highest. Compared with CK, the other S treatments did not significantly promote biomass accumulation. The net photosynthetic rates and chlorophyll contents in sulfur treatments were higher than those observed in CK, and the highest observed net photosynthetic rate and chlorophyll contents occurred in the NaHSO₃ and Na₂S, respectively. Na₂S₃ and Na₂SO₄ increased the activities of nitrogen and sulfur metabolism enzymes respectively compared to other treatments, with the CK being no inhibiting obvious in their effect. The responses of nutritional components to sulfur forms differed. Soluble protein and soluble amino acid concentrations were decreased in CK plants compared to S-treated plants, with the highest soluble protein, free amino acid, and soluble sugar concentrations observed in the NaHSO₃, Na₂SO₄ and NaHSO₃ treatments, respectively. In contrast to the physiological responses, the levels of secondary metabolites indigo and indirubin were the highest in Na₂S treatment. Considering both growth stimulation and secondary metabolite accumulation, the Na₂SO₄ treatment is recommended as the most beneficial in the cultivation of I. indigotica seedlings.

Key words: sulfur form, Isatis indigotica, photosynthetic physiology, nitrogen and sulfur metabolism, nutritional components, secondary metabolites

缪雨静, 关佳莉, 曹艺雯, 沙俊涛, 唐晓清, 耿丽, 王磊. 硫素形态对苗期菘蓝生长生理特性及次生代谢的影响[J]. 草业学报, 2019, 28(3): 101-110.

MIAO Yu-jing, GUAN Jia-li, CAO Yi-wen, SHA Jun-tao, TANG Xiao-qing, GENG Li, WANG Lei. The effect of sulfur forms on growth, physiology and secondary metabolism of Isatis indigotica at the seedling stage[J]. Acta Prataculturae Sinica, 2019, 28(3): 101-110.

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