马铃薯贮藏期主要病害拮抗内生细菌的筛选、鉴定及功能评价

doi:10.11686/cyxb20140332

摘要/Abstract

摘要： 马铃薯坏疽病、炭疽病和枯萎病是甘肃省马铃薯贮藏期最主要的病害,为了获得马铃薯贮藏期病害防治的生防菌株,利用对峙培养法从东祁连山高寒草地线叶嵩草内生细菌中筛选获得高抗菌株263XY1,其对马铃薯枯萎病菌、马铃薯炭疽病菌和马铃薯坏疽病菌的抑菌率分别达到67.17%,86.17%和70.89%;该菌株在含有色氨酸和不含色氨酸的金氏培养基中分泌的IAA分别为3.31和1.52 mg/L,在PKO含无机磷培养基中的有效磷增量为38.94 mg/L,具有固氮能力。经16S rDNA序列分析,其与死谷芽孢杆菌(Bacillus vallismortis,登录号:JQ547633.1)同源性达99%,初步鉴定为死谷芽孢杆菌。

Abstract: Phoma foveata, Colletotrichum coccodes and Fusarium avenaceum are 3 species of potato storage pathogens. The highly resistant bacterial strain 263XY1 tested against potato storage diseases was screened from endophytic bacteria isolated from Kobreasia capillifolia under alpine grassland in the Eastern Qilian Mountains using the confront culture method. The inhibition rates of 263XY1 against F. avenaceum, C. coccodes and P. foveata were 67.17%, 86.17% and 70.89%, respectively. The concentration of IAA secreted by 263XY1 was 3.31 mg/L in the King medium with Trp, and 1.52 mg/L without Trp. The increment of available P was 38.94 mg/L obtained by dissolving inorganic P (phosphate of calcium) and bacterial strain 263XY1 possessed the ability to fix nitrogen. The 16S rDNA sequence analysis indicated that 263XY1 shared 99% homology with Bacillus vallismortis (GenBank accession No. JQ547633.1), so 263XY1 was identified as B. vallismortis.

中图分类号:

S435.32

王颖,王玉琴,杨成德,姚玉玲,陈秀蓉,薛莉. 马铃薯贮藏期主要病害拮抗内生细菌的筛选、鉴定及功能评价[J]. 草业学报, 2014, 23(3): 269-275.

WANG Ying,WANG Yu-qin,YANG Cheng-de,YAO Yu-ling,CHEN Xiu-rong,XUE Li. Screening, identification and biological function evaluation of endophytic bacteria against potato storage disease[J]. Acta Prataculturae Sinica, 2014, 23(3): 269-275.

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