深绿木霉对白三叶草促生作用及生理生化特性的影响

doi:10.11686/cyxb20150218

摘要/Abstract

摘要： 通过室内种子萌发和温室幼苗盆栽试验研究了不同稀释倍数深绿木霉发酵液对白三叶草促生作用及生理生化特性的影响。结果表明,在室内试验条件下不同稀释倍数深绿木霉发酵液能够显著提高白三叶草种子的发芽率、发芽指数和活力指数,尤其100倍稀释液对种子发芽率、发芽指数和活力指数的影响较为明显,分别为94.42%,13.42和9.52。在温室条件下不同稀释倍数深绿木霉发酵液对白三叶草幼苗的生长具有明显的影响,并能够增加幼苗叶绿素、可溶性蛋白质含量和生理生化酶的活性,其中100倍发酵液处理后幼苗的根长、株高、植株鲜重、干重和根冠比的相对增长率分别为37.09%,13.18%,57.73%,54.35%,20.89%;叶绿素和可溶性蛋白质含量的相对增长率分别为14.02%和76.21%;多酚氧化酶、过氧化物酶和苯丙氨酸解氨酶活性均显著高于对照,其中多酚氧化酶活性在处理后第3天达到最大值,过氧化物酶活性在处理后第1和4天达到最大值,苯丙氨酸解氨酶活性在处理后第3~4天达到最大值。因此,深绿木霉发酵液对白三叶草的生长具有较强的促进作用。

Abstract: The effects of Trichoderma aureoviride fermentation on the growth and physiological characteristics of Trifolium repens were evaluated by seed germination in vitro and by pot experiments in a greenhouse. T. aureoviride fermentation was applied with different dilution rates and all dilution rates significantly increased the rates of seed germination, the germination index and the vigor index. The 100-times dilution had the most significant effects on seed germination rates, germination index and vigor index (94.42%, 13.42 and 9.52 respectively). In the greenhouse experiments, the fermentation of T. aureoviride with different dilution rates significantly promoted the growth of T. repens seedlings and significantly increased chlorophyll and soluble protein content, as well as the activities of antioxidant enzymes. The 100-times dilution increased T. repens root length, plant height, fresh weight, dry weight and root-shoot ratio by 37.09%, 13.18%, 57.73%, 54.35% and 20.89%respectively. It increased chlorophyll and soluble protein contents by 14.02% and 76.21% respectively, and significantly increased polyphenol oxidase (PPO), peroxidase (POD) and phenylalanine (PAL) enzyme activities. The maximum activity of PPO, POD and PAL presented at 3 days after treatment, 1 and 4 days after treatment and 3 and 4 days after treatment, respectively. These results suggest that the fermentation of T. aureoviride has significant growth promoting effects on T. repens.

张树武, 徐秉良, 程玲娟, 薛应钰. 深绿木霉对白三叶草促生作用及生理生化特性的影响[J]. 草业学报, 2015, 24(2): 161-167.

ZHANG Shuwu, XU Bingliang, CHENG Lingjuan, XUE Yingyu. Effects of Trichoderma aureoviride fermentation on the growth and physiological characteristics of Trifolium repens[J]. Acta Prataculturae Sinica, 2015, 24(2): 161-167.

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