欢迎访问《草业学报》官方网站,今天是 分享到:

草业学报

• 研究简报 • 上一篇    

饲料添加苜蓿草粉对刺参幼参生长、体成分及免疫酶的影响

宋坚, 何舟, 程龙, 庞云龙, 宁军号, 常亚青*   

  1. 大连海洋大学水产与生命学院,农业部北方海水增养殖重点实验室,辽宁 大连 116023
  • 收稿日期:2013-12-23 出版日期:2015-02-20 发布日期:2015-02-20
  • 作者简介:宋坚(1971-),男,山东青岛人,副研究员。E-mail:dlmel@163.com
  • 基金资助:
    辽宁省农业攻关计划重大项目(2011203003)资助

Effects of alfalfa meal on growth, body composition and immune enzymes in Apostichopus japonicus

SONG Jian, HE Zhou, CHENG Long, PANG Yunlong, NING Junhao, CHANG Yaqing*   

  1. College of Fisheries and Life Science, Dalian Ocean University, Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian 116023, China
  • Received:2013-12-23 Online:2015-02-20 Published:2015-02-20

摘要:

为了探究紫花苜蓿草粉的适宜添加比例,研究了苜蓿草粉的添加水平依次为0(对照),5%(A1),10%(A2),15%(A3)和20%(A4) 的5种饲料对刺参幼参(3.16±0.66) g生长、体成分和免疫酶活性的影响。结果显示,刺参特定生长率(SGR)的最大值(0.73%)和饵料系数(FCR)的最小值都出现在A2组。A2组刺参的粗蛋白含量最高(52.10%),显著高于A1与A4组(P<0.05)。A4组刺参的过氧化氢酶(CAT)活力值最高(142.28 U/mL),显著高于A0、A1与A2组(P<0.05);A3组刺参的超氧化物歧化酶(SOD)活力值最高(136.58 U/mL),显著高于A0组(P<0.05); A3组刺参的溶菌酶(LZM)含量最高(1.92 μg/mL),显著高于A0、A1与A2组(P<0.05)。结果表明,当苜蓿草粉的添加比例为10%左右时,实验刺参的养殖效果最佳。

Abstract:

To determine the appropriate proportion of Medicago sativa in the diet of Japanese sea cucumber (Apostichopus japonicus), the effects of five diets containing zero (A0), 5% (A1), 10% (A2), 15% (A3) and 20% (A4) M. sativa meal on growth, body composition and immune enzyme activity in A. japonicus (3.16±0.66 g) were assessed. The A2 diet produced the highest growth rate (0.73%), feed conversion ratio (FCR) and crude protein content (52.10%), significantly higher (P<0.05) than the A1 and A4 diets. The activity of catalase in sea cucumbers from group A4 (142.28 U/mL) was significantly higher than those in groups A0, A1 and A2 (P<0.05). Superoxide dismutase (SOD) activity in A3 (136.58 U/mL) was significantly higher than the A0 group (P<0.05). Additionally, lysozyme (LZM) content in the A3 group (1.92 μg/mL) was significantly higher than those in groups A0, A1 and A2 (P<0.05). The results suggest that the optimum proportion of M. sativa in A. japonicus diets is 10%.