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草业学报 ›› 2015, Vol. 24 ›› Issue (4): 87-94.DOI: 10.11686/cyxb20150410

• 研究论文 • 上一篇    下一篇

吸胀冷害下外源NO对紫花苜蓿种子萌发及抗氧化性的影响

赵萌,魏小红*   

  1. 甘肃农业大学生命科学技术学院,甘肃 兰州 730070
  • 收稿日期:2014-03-21 修回日期:2014-04-22 出版日期:2015-04-20 发布日期:2015-04-20
  • 通讯作者: 魏小红,E-mail:weixh@gsau.edu.cn
  • 作者简介:赵萌(1987-),女,陕西黄陵人,在读硕士。E-mail:741828038@qq.com
  • 基金资助:
    甘肃省自然基金(145RJZA196)资助。

Effects of nitric oxide on Medicago sativa seed germination under imbibitional chilling

ZHAO Meng, WEI Xiao-Hong*   

  1. School of Life Science & Technology, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2014-03-21 Revised:2014-04-22 Online:2015-04-20 Published:2015-04-20

摘要: 用浓度为0.1 mmol/L外源NO供体硝普钠(sodium nitroprusside,SNP)处理4℃吸胀冷害下紫花苜蓿种子,来探讨吸胀冷害下NO对紫花苜蓿种子萌发及氧化损伤的影响。试验设计4个处理,分别为A:对照组(CK)为蒸馏水;B:4℃吸胀冷害组(Chilling);C: SNP;D:Chilling+SNP,每个处理重复3次。结果表明:1)紫花苜蓿种子经SNP处理12 h转入25℃继续培养84 h后,Chilling+SNP组比Chilling组发芽率、活力指数、发芽指数提高了14.37%,34.17%,1.29%。2)4℃吸胀冷害12 h后,Chilling+SNP组同Chilling组相比超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性分别提高了149.26%,60.35%,过氧化氢(H2O2)含量及超氧阴离子(·O2-)产生速率则降低了10.11%,27.61%。3)SNP对4℃吸胀冷害12,24和48 h 的种子体内羟自由基(·OH)的清除均呈下降趋势,Chilling+SNP组的清除率均高出SNP组,Chilling+SNP组在冷害12 h时清除率达最大值97.5%。综上所述,施加外源NO能促进种子的萌发,提高抗氧化酶活性,降低了活性氧对种子造成的伤害。

Abstract: Seeds of Medicago sativa were used to study the effects of 0.1 mmol/L sodium nitroprusside (SNP), as a source of nitric oxide, on seed germination and antioxidant capacity under imbibitional chilling at 4℃. Four treatments: control, chilling, SNP and chilling+SNP were replicated three times. The results indicated that after imbibitional chilling for 12 h and germination at 25℃ for 84 h, the vigor and germination of M. sativa seeds were increased by SNP treatment. The activities of antioxidant enzymes including superoxide dismutase (SOD) and catalase (CAT) increased 149.3% to 60.4% by SNP treatment. The radical clearance rate tended to decline with SNP treatments and chilling for 12, 24 and 48 h. At 12 h chilling the radical clearance rate reached a maximum of 97.5%. Imposing exogenous nitric oxide can promote germination, enhance antioxidant enzyme activities and reduce the reactive oxygen damage in M. sativa seed.