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草业学报 ›› 2013, Vol. 22 ›› Issue (5): 128-135.DOI: 10.11686/cyxb20130515

• 研究论文 • 上一篇    下一篇

四种宿主植物及其不同栽培密度对AM真菌扩繁的影响

李媛媛,王晓娟,豆存艳,林双双,罗巧玉,崔慧君,孙莉,金樑*   

  1. 兰州大学草地农业生态系统国家重点实验室 草地农业科技学院,甘肃 兰州 730020
  • 出版日期:2013-10-20 发布日期:2013-10-20
  • 通讯作者: E-mail:liangjin@lzu.edu.cn
  • 作者简介:李媛媛(1988-),女,河南焦作人,在读硕士。
  • 基金资助:
    国家自然科学基金项目(31270558),农业部公益性行业(农业)科研专项(201203041)和兰州大学中央高校基本科研业务费专项资金(lzujbky-2013-86)资助。

Effects of four host plants and different cultivation densities on the propagation of arbuscular mycorrhizal fungi

  1. State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
  • Online:2013-10-20 Published:2013-10-20

摘要: 为了有效的构建丛枝菌根(arbuscular mycorrhizal,AM)真菌的高效繁殖体系,本研究利用盆栽培养法研究玉米、高粱、青葱和三叶草等4种不同的宿主植物及其不同的栽培密度对AM真菌地表球囊霉(Glomus versiforme)扩繁的影响。结果表明,温室条件下,4种宿主植物均可与AM真菌形成共生体,宿主植物种类和栽培密度对生物量、侵染率、孢子密度等均产生显著影响(P<0.05),除白三叶的地径和高粱的叶片数外所有植物的生长参数指标在高密度(10株/盆)栽培下均低于低密度(5株/盆)处理,但10株/盆处理的AM真菌侵染率和孢子密度均显著高于5株/盆(P<0.05)。本实验表明不同的宿主植物与AM真菌的共生状况不同,选择生物量大的玉米,并采用适度的密植栽培虽然会导致对宿主植物生长的不利条件,但却可以增加AM真菌的孢子产量,适合于AM真菌扩繁体系的构建。

Abstract: To establish a propagation system for arbuscular mycorrhizal (AM) fungi, four different plants: maize (Zea mays), sorghum (Sorghum bicolor), white clover (Trifolium repens) and green onion (Allium fistulosum) were selected for this study. Two different cultivation densities were used to test the influence of plant densities on the propagation of an AM fungus (Glomus versiforme). Different plant species and different densities had a significant influence on the colonization and spore production of G. versiforme and on the biomass of host plants (P<0.05). The extent of AM colonization and spore densities at 10 plants per pot were much higher than at 5 plants per pot (P<0.05). Our studies suggest that maize, which has the highest biomass with high density, was suitable for propagation of G. versiforme.

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