干旱胁迫对金心吊兰叶片活性氧及其清除系统的影响

doi:10.11686/cyxb20130529

草业学报 ›› 2013, Vol. 22 ›› Issue (5): 248-255.DOI: 10.11686/cyxb20130529

干旱胁迫对金心吊兰叶片活性氧及其清除系统的影响

贾学静,董立花,丁春邦,李旭,袁明^*

四川农业大学生命科学与理学院,四川雅安 625014

出版日期:2013-10-20 发布日期:2013-10-20
通讯作者: E-mail:yuanmingsicau@yahoo.com.cn
作者简介:贾学静(1988-),男,四川广元人,在读硕士。
基金资助:
四川省教育厅青年基金项目(11ZB054)资助。

Effects of drought stress on reactive oxygen species and their scavenging systems in Chlorophytum capense var. medio-pictum leaf

JIA Xue-jing, DONG Li-hua, DING Chun-bang, LI Xu, YUAN Ming

Faculty of Biology and Science, Sichuan Agricultural University, Ya’an 625014, China

Online:2013-10-20 Published:2013-10-20

摘要/Abstract

摘要： 以金心吊兰叶片边缘的绿色区域与中心黄白色区域为材料,研究了干旱胁迫对其活性氧及其清除系统的影响,结果表明,1)随着胁迫时间的延长,叶片各区域的过氧化氢(H₂O₂)、超氧阴离子(O₂^-·)含量和丙二醛(MDA)含量均表现出逐渐上升的趋势,而超氧化物歧化酶(SOD)与过氧化物酶(POD)活性则先上升后下降。2)随着胁迫浓度的增加,叶片中活性氧(H₂O₂,O₂^-·)含量、抗氧化酶(SOD,POD)活性和MDA含量表现出上升的趋势。3)采用隶属函数法对吊兰叶片的抗旱性进行了综合评价,其抗旱性表现出位置效应,叶片中心区域抗旱性强于边缘区域。4)吊兰叶片的抗旱性与叶绿素含量呈负相关,叶绿素含量相对高的区域其抗旱性相对较低,金心吊兰抗旱性强于野生型。

Abstract: The effects of drought stress on reactive oxygen species and their scavenging systems of leaf blade and the center parts of Chlorophytum capense var. medio-pictum were studied. 1) The content of H₂O₂, O₂^-· and MDA gradually increased with the stress time, while the activity of SOD and POD increased in the earlier days, but then declined. 2) The content of reactive oxygen species (H₂O₂ and O₂^-·), activity of scavenging enzymes (SOD and POD) and the content of MDA increased gradually as the PEG concentration increased. 3) The comprehensive evaluation of drought tolerance by the membership function method indicated that there were clear position effects, and drought resistance in the center part of the leaf was stronger than that in the blade of the leaf. 4) Drought resistance was negatively correlated with chlorophyll content: the higher the chlorophyll content, the weaker the drought tolerance. Drought resistance in C. capense var. medio-pictum was better than that of C. capense.

中图分类号:

贾学静,董立花,丁春邦,李旭,袁明. 干旱胁迫对金心吊兰叶片活性氧及其清除系统的影响[J]. 草业学报, 2013, 22(5): 248-255.

JIA Xue-jing, DONG Li-hua, DING Chun-bang, LI Xu, YUAN Ming. Effects of drought stress on reactive oxygen species and their scavenging systems in Chlorophytum capense var. medio-pictum leaf[J]. Acta Prataculturae Sinica, 2013, 22(5): 248-255.

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干旱胁迫对金心吊兰叶片活性氧及其清除系统的影响

Effects of drought stress on reactive oxygen species and their scavenging systems in Chlorophytum capense var. medio-pictum leaf

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