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草业学报 ›› 2013, Vol. 22 ›› Issue (3): 259-.DOI: 10.11686/cyxb20130334

• 研究论文 • 上一篇    下一篇

钝裂银莲花不同居群遗传多样性的ISSR分析

孙涛,刘左军*,刘凤梅,伍国强,王永刚,李冰   

  1. 兰州理工大学生命科学与工程学院,甘肃 兰州730050
  • 出版日期:2013-06-20 发布日期:2013-06-20
  • 通讯作者: E-mail:zuojunl@lut.cn
  • 作者简介:xiaoyaodidai@126.com
  • 基金资助:
    国家自然科学基金项目(30960066)资助。

Analysis of genetic diversity in Anemone obtusiloba populations with ISSR markers

SUN Tao, LIU Zuo-jun, LIU feng-mei, WU Guo-qiang, WANG Yong-gang, LI Bin   

  1. Analysis of genetic diversity in Anemone obtusiloba populations with ISSR markers
    SUN Tao, LIU Zuo-jun, LIU feng-mei, WU Guo-qiang, WANG Yong-gang, LI Bin
    (School of Life Science and Engineering, Lanzhou University of Science and
    Technology, Lanzhou 730050, China)
  • Online:2013-06-20 Published:2013-06-20

摘要: 本研究采用ISSR分子标记技术分析了甘肃省4个地区(合作、碌曲、阿万仓和阿孜)钝裂银莲花的11个居群的遗传多样性水平和遗传结构。结果表明,13个引物共扩增出701条带,其中392条具有多态性,多态位点百分率为55.92%。在物种水平上,Shannon多样性指数(I)为0.372,Nei基因多样性指数(H)为0.250;Nei的基因分化系数Gst和AMOVA分析表明钝裂银莲花居群遗传分化大部分存在于居群间。在居群水平上,I为0.183,H为0.114,可见钝裂银莲花居群具有较高的遗传多样性。依据Nei的遗传距离对不同居群进行UPGMA聚类,聚类结果为合作居群为一类,碌曲和阿万仓居群为一类,阿孜为一类。居群遗传多样性与环境因子间的相关性分析表明,遗传多样性水平与海拔、含水量、有效磷和经纬度之间没有相关性,而与降水量之间显著负相关。

Abstract: Eleven species of Anemone obtusiloba collected from 4 areas of Gansu were analysed by inter single sequence repeats (ISSR) to elucidate the inherit diversity level and structure. Seven hundred and one bands were amplified by 13 primers and 392 bands were polymorphic, a polymorphic locus percentage (P) of 55.92%. The Shannon index (I) was 0.372 and Nei’s gene diversity (H) was 0.250. Based on Nei’s Gst value and analysis of molecular variance (AMOVA), showed that species inheritance of differentiation of A. obtusiloba existed mainly. In inter-populations: I was 0.183 and H was 0.114 at the population level. These data indicated that there were high inherent diversities in the population of A. obtusiloba. Using UPGMA, three populations were clustered: HZ, LQ and AWC, and AZ. Further analysis showed that there was no correlation between population genetic diversity level and altitude, water, extractable phosphorous or longitude and latitude, but there was a significantly negative correlation with precipitation.

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