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草业学报 ›› 2013, Vol. 22 ›› Issue (3): 250-.

• 研究论文 • 上一篇    下一篇

抗逆转录因子在百脉根中的功能分析

陶飞1,2,马江涛2,唐益雄2,李京生3,卢运明3,徐秉良1*,吴燕民2*   

  1. 1.甘肃农业大学草业学院 草业生态系统教育部重点实验室 甘肃省草业工程实验室 中-美草地畜牧业可持续发展研究中心,甘肃 兰州 730070;
    2.中国农业科学院生物技术研究所, 北京 100081;
    3.深圳市农科集团有限公司,广东 深圳 518040
  • 出版日期:2013-06-20 发布日期:2013-06-20
  • 通讯作者: E-mail:xubl@gsau.edu.cn;Wuym65@sina.com
  • 作者简介:skyfit-1985@163.com
  • 基金资助:
    “973”项目(2007CB108902-2)资助。

Functional analysis of drought-resistant transcription factors PeDREB2a
and HhERF2 in Lotus corniculatus

TAO Fei1,2, MA Jiang-tao2, TANG Yi-xiong2, LI Jing-sheng3, LU Yun-ming3, XU Bing-liang1, WU Yan-min2   

  1. Functional analysis of drought-resistant transcription factors PeDREB2a
    and HhERF2 in Lotus corniculatus
    TAO Fei1,2, MA Jiang-tao2, TANG Yi-xiong2, LI Jing-sheng3,
    LU Yun-ming3, XU Bing-liang1, WU Yan-min2
    (1.College of Grassland Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem,
    Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S.
    Centers for Grazingland Ecosystem Sustainability, Lanzhou 730070, China;
    2.Biotechnology Research Institute, Chinese Academy of Agricultural
    Sciences, Beijing 100081, China; 3.Shenzhen Nongke Group,
    Shenzhen 518040, China)
  • Online:2013-06-20 Published:2013-06-20

摘要: 为提高豆科牧草百脉根抗旱方面的能力,从沙生植物胡杨和铃铛刺中克隆了4个抗逆转录因子基因并进行功能分析,从中筛选了2个具有抗旱特性的基因PeDREB2aHhERF2,构建了以磷酸甘露糖异构酶为筛选标记的单、双价植物表达载体,转化百脉根Leo品种,结果表明,转录因子基因明显改善了百脉根的抗旱特性,经过抗旱复水试验证明转基因百脉根成活率达到100%。

Abstract: Two drought-resistant genes (PeDREB2a and HhERF2) were identified through functional analysis of the four transcriptional factor genes cloned from Populus euphraticaand Halimodendron halodendron, respectively. PeDREB2a and HhERF2 were two factors which had positive effects in plants against drought stress. Based on this, a series of plant expression vectors either harboring PeDREB2a or HhERF2 were constructed with a non-resistant marker gene and transferred into the Lotus genome using an Agrobacterium mediated method. Transgenic Lotus had strong tolerance to drought. This showed that transcription factors significantly improved drought resistance of L. corniculatus, and the survival rate of transgenic L. corniculatus was 100% in a drought recovery water test.

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