磷肥对紫花苜蓿再生过程中根茬组织非结构性碳水化合物利用的影响

草业学报 ›› 2009, Vol. 18 ›› Issue (3): 136-141.

磷肥对紫花苜蓿再生过程中根茬组织非结构性碳水化合物利用的影响

姜慧新^1,2,沈益新^1*,翟桂玉²,刘信宝¹,原培勋²

1.南京农业大学动物科技学院, 江苏南京 210095;
2.山东省畜牧总站, 山东济南 250022

收稿日期:2008-07-07 出版日期:2009-06-20 发布日期:2009-06-20
作者简介:姜慧新(1974-),女,浙江金华人,高级畜牧师,在读博士。E-mail: jhx232@sina.com
基金资助:
山东省农业良种产业化项目(2005LZ13-03,2006LZ12-03)资助。

Effects of phosphate fertilizer on utilization of non-structural carbohydrate
in resident tissue of Medicago sativa during the early regrowth stage

JIANG Hui-xin^1,2, SHEN Yi-xin¹, ZHAI Gui-yu², LIU Xin-bao¹, YUAN Pei-xun²

1.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;

2.General Station of Animal Husbandry of Shandong Province, Ji’nan 250022, China

Received:2008-07-07 Online:2009-06-20 Published:2009-06-20

摘要/Abstract

摘要： 田间试验研究了施磷肥对紫花苜蓿再生过程中主根、根冠和残茬中非结构性碳水化合物(NSC)贮存量变化的影响。结果表明,紫花苜蓿NSC主要贮存在主根和根冠部,主根和根冠中的淀粉是再生过程中被利用最多的NSC。施磷肥显著提高了刈割时主根和根冠部淀粉的贮存量,270 kg P₂O₅/hm² 处理的植株主根和根冠部淀粉贮存量分别比0 kg P₂O₅/hm² 处理的植株高73.5% 和31.1% ;施磷肥处理提高了主根中α-淀粉酶的活性,加快了主根和根冠部贮存淀粉在再生早期的降解,提高了主根和根冠部淀粉转化为再生生物量的效率,促进了休眠芽的萌发和新生茎的生长。270 kg P₂O₅/hm² 处理的植株刈割后13 d的每株再生生物量极显著高于不施磷处理的植株(P＜0.01)。结果说明,紫花苜蓿主根和根冠部贮存的NSC是再生的主要营养源;施磷肥通过提高紫花苜蓿根茬组织中NSC的贮存量和利用效率,为刈割后的早期再生提供充足的养分,从而加快紫花苜蓿的再生。

Abstract: A field experiment was conducted to study the effects of phosphate fertilizer on utilization of non-structural carbohydrate (NSC) stored in the main root, crown, and stubble of alfalfa (Medicago sativa) during early regrowth after defoliation. NSC is mainly stored in the root and crown. A great deal of starch, the major component of stored NSC, was utilized during early regrowth. With increasing rates of phosphate fertilizer, starch reserves increased in the main root and crown and at 270 kg P₂O₅/ha starch reserves were 73.5% and 31.1% higher respectively than those in the control. Phosphate fertilizer improved the activity of α-amylase and accelerated the hydrolyzis of starch in the main root and crown, thus promoting regrowth by enhancing mobilization of starch reserves for emergence of dormant buds and new shoots after defoliation. The dry weight of plants treated with 270 kg P₂O₅/ha was significantly (P＜0.01) greater than the control 13 days after defoliation. NSC in the main root and crown of alfalfa is the main source of nutrients during early regrowth and phosphate fertilizer improved the storage and conversion efficiency of NSC thus accelerated regrowth of alfalfa after defolition.

中图分类号:

姜慧新,沈益新,翟桂玉,刘信宝,原培勋. 磷肥对紫花苜蓿再生过程中根茬组织非结构性碳水化合物利用的影响[J]. 草业学报, 2009, 18(3): 136-141.

JIANG Hui-xin, SHEN Yi-xin, ZHAI Gui-yu, LIU Xin-bao, YUAN Pei-xun. Effects of phosphate fertilizer on utilization of non-structural carbohydrate
in resident tissue of Medicago sativa during the early regrowth stage[J]. Acta Prataculturae Sinica, 2009, 18(3): 136-141.

参考文献

[1]戎郁萍,韩建国,王培,等. 刈割强度对新麦草产草量和贮藏碳水化合物及含氮化合物影响的研究[J[. 中国草地,2000,(2):28-34.
[2]Donaghy D J, Fulkerson W J. Priority for allocation of water-soluble carbohydrate reserves during regrowth of Lolium perenne[J[. Grass Forage Science, 1998, 53: 211-218.
[3]Gonzalez B, Boucaud J, Salette J, et al. Changes in stubble carbohydrate content during regrowth of defoliated perennial ryegrass (Lolium perenne L.)on two nitrogen levels[J[. Grass and Forage Science, 1989, 44: 411-415.
[4]王静,杨持,王铁娟,等.不同刈割强度下冷篙再生生长与可溶性碳水化合物的相关分析[J[. 内蒙古大学学报,2004,35(2):177-182.
[5]Yoki A, Manabu T, Masataka S, et al. Amylase activities and carbohydrate content in stems and roots of phasey bean (Macroptilium lathyriodes (L.) Urb) after defoliation[J[. Grassland Science, 2003, 49(1): 16-22.
[6]Gallagher J A, Volenec J J, Turner L B, et al. Starch hydrolytic enzyme activities following defoliation of white clover[J[. Crop Science, 1997, 37: 1812-1818.
[7]Jeffrey J, Volence J J, Patricia J, et al. Carbohydrate metabolism in taproots of Medicago sative L. during winter adaptation and spring regrowth[J[. Plant Physiology, 1991, 96: 786-793.
[8]李富宽,翟桂玉,沈益新,等. 施磷和接种根瘤菌对黄河三角洲紫花苜蓿生长及品质的影响[J[. 草业学报,2005,14(3):87-93.
[9]Matt A S, Ronald M J. Stand dynamics and yield components of alfalfa as affected by phosphorus fertility[J[. Agronomy Journal, 1993, 85: 241-246.
[10]Berg W K, Cunningharm S M, Brouder S M, et al. Influence of phosphorus and potassium on alfalfa yield and yield components[J[. Crop Science, 2005, 45: 297-304.
[11]Li R, Volence J J, Joern B C, et al. Effects of phosphorus nutrition on carbohydrate and protein metabolism in alfalfa roots[J[. Journal of Plant Nutrition, 1998, 21(3): 459-474.
[12]李合生.植物生理生化原理和技术[M[.北京: 高等教育出版社,2000.169-172.
[13]张光辉,李增嘉,潘庆民,等. 内蒙古典型草原羊草和大针茅地下器官中碳水化合物含量的季节性变化[J[. 草业学报,2006,15(3):42-49.
[14]TA T C, Macdowall F D, Faris M A. Utilization of carbon and nitrogen reserves of alfalfa roots in supporting N₂-fixation and shoot regrowth[J[. Plant and Soil, 1990, 127: 231-236.
[15]Johansson G. Carbon distribution in grass (Festuca pratensis L.) during regrowth after cutting-utilization of stored and newly assimilated carbon[J[. Plant and Soil, 1993, 151:11-20.
[16]干友民, Schnyder H, Vianden H, 等. 多年生黑麦草刈后再生草碳水化合物及氮素的变化[J[. 草业学报,1999,8(4):65-70.
[17]郭程瑾,李宾兴,王斌,等. 不同磷效率小麦品种的光合特性及其生理机制[J[. 作物学报,2006,32(8):1209-1217.
[18]李贤勇,任天举,邹亚兰. 不同时期施磷钾肥对杂交中稻-再生稻的影响研究[J[. 再生稻,1995,(1):30-35.