不同生态型芦苇生境适应性的遗传差异

草业学报 ›› 2009, Vol. 18 ›› Issue (5): 250-255.

不同生态型芦苇生境适应性的遗传差异

刘斌斌,刑家强,龚晓洁,浦铜良^*

兰州大学生命科学学院植物与植物生理研究所,甘肃兰州 730000

收稿日期:2008-12-15 出版日期:2009-10-20 发布日期:2009-10-20
作者简介:刘斌斌(1985-),男,甘肃平凉人,在读硕士。E-mail: liubb06@lzu.cn
基金资助:
国家自然科学基金(30470163)资助。

Genetic differences of different reed ecotypes adapted to individual habitats

LIU Bin-bin, XING Jia-qiang, GONG Xiao-jie, PU Tong-liang

School of Life Science, Lanzhou University, Lanzhou 730000, China

Received:2008-12-15 Online:2009-10-20 Published:2009-10-20

摘要/Abstract

摘要： 以甘肃省临泽县内的4种生态型芦苇为材料,采用随机扩增多态DNA技术和等位酶多态性分析技术初步探讨了不同生态型芦苇对各自生境适应性的遗传差异。RAPD-PCR(随机扩增多态DNA聚合酶链式反应)采用10碱基随机引物,筛选出16条重复性高并产生明显多态性产物的引物,共扩增了559条谱带,其中489条谱带具有多态性,多态性百分数为87.5%。等位酶分析选用了12个等位酶系统,其中9个基因位点显示了多态性,多态位点平均百分数为34.1%。试验结果表明,不同生态型芦苇之间具有显著的分子遗传差异和等位酶表达差异,同时属于同一生态型的个体和群体之间的遗传差异很小,这与不同生态型芦苇在适应各自生境的过程中所形成的形态学和生理学方面的差异是一致的,说明各个生态型之间的差异是遗传分化的结果。

Abstract: The genetic differences among different reed ecotypes was analysed on four ecotypes of reed (Phragmites communis) in Linze County, Gansu Province, using RAPDs and allozyme genetic diversity analysis. Ten base random primers were used in the RAPD analysis and 16 primers were chosen on the basis of repeatedly forming significant polymorphism products. Of a total of 559 amplification bands 489 (87.5%) were polymorphic. Using 12 allozyme loci, allozyme genetic diversity analysis, showed there were nine gene sites with polymorphisms. The average percentage of polymorphic sites was 34.1%. The Results indicated that notable molecular genetic diversity and different ecotype reed allozyme expression divesity existed among the different ecotypes but genetic diversity between individuals of the same ecotype was slight. This is consistent with the distinct differences between the habitats and together with the morphological and physiological variations, it demonstrates that these differences are involved in different reed ecotype genetic differentiation.

中图分类号:

刘斌斌,刑家强,龚晓洁,浦铜良. 不同生态型芦苇生境适应性的遗传差异[J]. 草业学报, 2009, 18(5): 250-255.

LIU Bin-bin, XING Jia-qiang, GONG Xiao-jie, PU Tong-liang. Genetic differences of different reed ecotypes adapted to individual habitats[J]. Acta Prataculturae Sinica, 2009, 18(5): 250-255.

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