甘肃境内鼢鼠Eospalax亚属分子系统发育研究

草业学报 ›› 2009, Vol. 18 ›› Issue (6): 204-209.

甘肃境内鼢鼠Eospalax亚属分子系统发育研究

杨红善¹,何小琴²,刘荣堂³,张三亮³,常根柱¹

1.中国农业科学院兰州畜牧与兽药研究所,甘肃兰州 730050;
2.甘肃省林业调查规划设计研究院,
甘肃兰州 730020;
3.甘肃农业大学草业学院,甘肃兰州 730070

收稿日期:2008-12-22 出版日期:2009-12-20 发布日期:2009-12-20
作者简介::杨红善(1981-),男,甘肃兰州人,硕士。E-mail:yanghsh123@126.com
基金资助:
全球环境基金项目(GEF)资助。

Molecular phylogenesis of the Eospalax subgenus in the Gansu province

YANG Hong-shan¹, HE Xiao-qin², LIU Rong-tang³, ZHANG San-liang³, CHANG Gen-zhu¹

1.Lanzhou Institute of Animal &；
Veterinary Pharmaceutics Sciences, CAAS, Lanzhou 730050, China；

2.Foresty Investing and Planning Institute, Lanzhou 730020, China；
3.College of Grassland
Science, Gansu Agriculturcal University, Lanzhou 730070, China

Received:2008-12-22 Online:2009-12-20 Published:2009-12-20

摘要/Abstract

摘要： 为了筛选评价甘肃境内鼢鼠凸颅亚属母性遗传多样性分子标记,研究该亚属的分子系统发育。测定了分布于甘肃11个不同地方31只属于鼢鼠中凸颅亚属的不同鼢鼠,其中包括:9只高原鼢鼠、9只甘肃鼢鼠、9只斯氏鼢鼠、4只秦岭鼢鼠的细胞色素b基因全序列1 140 bp。从GenBank获得包括平颅亚属在内的21个细胞色素b全序列总共52条全序列,用MEGA软件分别绘制出本研究的31条全序列和总共52条全序列的2个系统发育树。运用测定序列,结合相关软件分析31条细胞色素b基因全序列的变化特征;分析和探讨本试验的Eospalax亚属4个种的分类及系统发育。本研究为鼢鼠的系统发育提供了分子理论依据。

Abstract: Maternity genetic diversity of the subgenus Eospalax in Gansu province was evaluated by studying the molecular phylogenesis of this subgenus. The nucleotide complete sequence variations of mitochondrial DNA (mtDNA) and cytochrome B gene (1 140 bp) were examined. Sequence data were obtained from 31 samples of the subgenus Eospalax from 11 different places in Gansu province and 21 other sequences were acquired from GenBank. Four Gansu species were Qinling zokor, Gansu zokor, Bailey’s zokor, and Smith’s zokor. Two phylogenetic trees were constructed, one from the 31 near complete Cytb sequences from our experiment and 52 Cyt which were aligned with GeneBank. Using these results and related software, we analysed the change of Cytb gene sequences and discussed the classification and phylogenesis of four species of the subgenus Eospalax. This study provides the molecular basis for the phylogenesis of the subgenus Eospalax.

中图分类号:

杨红善,何小琴²,刘荣堂³,张三亮³,常根柱. 甘肃境内鼢鼠Eospalax亚属分子系统发育研究[J]. 草业学报, 2009, 18(6): 204-209.

YANG Hong-shan, HE Xiao-qin, LIU Rong-tang, ZHANG San-liang, CHANG Gen-zhu. Molecular phylogenesis of the Eospalax subgenus in the Gansu province[J]. Acta Prataculturae Sinica, 2009, 18(6): 204-209.

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