外源性金属硫蛋白对奶牛泌乳性能和抗氧化状况的影响

草业学报 ›› 2009, Vol. 18 ›› Issue (6): 137-143.

外源性金属硫蛋白对奶牛泌乳性能和抗氧化状况的影响

张彬¹,谭琼¹,肖兵南³,李丽立^2*,陈宇光¹,肖定福¹,刘海林³,吴宗明¹,张复生²

1.湖南农业大学动物科学技术学院, 湖南长沙 410128;
2.中国科学院亚热带农业生态研究所,
湖南长沙 410125;
3.湖南省畜牧兽医研究所,湖南长沙 410131

收稿日期:2009-03-24 出版日期:2009-12-20 发布日期:2009-12-20
作者简介::张彬(1955-),男,湖南安仁人,教授,博士,博士生导师。E-mail:zhb8236@126.com
基金资助:
国家自然科学基金项目(30671516;30170684),湖南省自然科学基金重点项目(06JJ20091)和湖南省教育厅重点项目(08A029)资助。

Effects of exogenous metallothionein on milkability and anti-oxidative status in dairy cattle

ZHANG Bin¹, TAN Qiong¹, XIAO Bing-nan³, LI Li-li², CHEN Yu-guang¹,
XIAO Ding-fu¹, LIU Hai-lin³, WU Zong- ming¹, ZHANG Fu-sheng²

1.College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China；

2.Institute of Subtropical Agricultural Ecology, The Chinese Academy of Sciences, Changsha 410125,
China；
3.Institute of Animal Science and Veterinary of Hunan Province, Changsha 410131, China

Received:2009-03-24 Online:2009-12-20 Published:2009-12-20

摘要/Abstract

摘要： 选取24头中国荷斯坦奶牛经产泌乳母牛,随机等分为A、B、C、D四组,分别每头静脉注射经生理盐水溶解的Zn-MT 0 (对照),4.0,8.0和12.0 mg,以探讨外源性金属硫蛋白(MT)对奶牛泌乳性能和血清抗氧化状况的影响。结果表明,C和D组的4％标准乳产量均显著高于对照组(P<0.05),但各组间的乳脂率无显著差异(P>0.05)。B、C和D组血清谷胱甘肽过氧化物酶(GSH-Px)活性均显著高于对照组(P<0.05)。D组血清过氧化氢酶(CAT)活性分别显著高于B组(P<0.05)和对照组(P<0.01);C组血清CAT活性也显著高于对照组(P<0.05)。C和D组总抗氧化能力(T-AOC)分别显著高于对照组和B组(P<0.05)。B、C和 D组血清丙二醛(MDA)含量均显著低于对照组(P<0.05)。说明MT不失为一种能够有效调控奶牛泌乳量和抗氧化能力的生理活性物质和高效抗氧化剂。

Abstract: The effects of exogenous metallothionein (Zn-MT) on milkability and anti-oxidative status of dairy cattle was studied using 24 lactating Chinese Holstein cows. They were randomly allocated to groups A, B, C, and D, and Zn-MT was supplemented at 0, 4.0, 8.0 and 12.0 mg/capita respectively by intravenous injection. The milk yields of the cows in groups C and D were higher (P<0.05) than those in group A, but there were no significant differences in milk yields between groups A and B or in milkfat percentages between groups. The blood GSH-Px activities of the cows in groups B, C, and D were also higher(P<0.05)than that in group A. The blood CAT activities of the cows in group D were higher than those in groups A (P<0.01) and B (P<0.05), and there were significant differences (P<0.05) in the blood CAT activities of group A and C cows. The T-AOC levels of the cows in groups C and D were also higher (P<0.05) than those in groups A and B. The MDA serum content of cows in groups B, C, and D were lower (P<0.05) than of cows in group A. Exogenous Zn-MT should be an effective anti-oxidative substance and physiological supplement for dairy cattle.

中图分类号:

S823.9⁺1

张彬,谭琼,肖兵南³,李丽立^2*,陈宇光,肖定福,刘海林³,吴宗明,张复生². 外源性金属硫蛋白对奶牛泌乳性能和抗氧化状况的影响[J]. 草业学报, 2009, 18(6): 137-143.

ZHANG Bin, TAN Qiong, XIAO Bing-nan, LI Li-li, CHEN Yu-guang,
XIAO Ding-fu, LIU Hai-lin, WU Zong- ming, ZHANG Fu-sheng. Effects of exogenous metallothionein on milkability and anti-oxidative status in dairy cattle[J]. Acta Prataculturae Sinica, 2009, 18(6): 137-143.

参考文献

[1] Romeroisart N, Vasak M. Advances in the structure and chemistry of metallothionein[J]. Journal of Inorganic Biochemistry, 2002, 88: 388-396.
[2] Palmiter R D. The elusive function of metallothioneins[J]. Proceedings of the National Academy of Sciences, USA, 1998, 95: 8428-8433.
[3] 郑军恒, 李海洋, 茹刚, 等. 金属硫蛋白清除羟自由基功能的研究[J]. 北京大学学报(自然科学版), 1999, 35(4): 573-576.
[4] Wang G W, Zhou Z X. Inhibition of hypoxi reoxygenation-induced apoptosis in metallothionein -overexpressing cardiomyoeytes[J]. American Journal of Physiology-Heart and Circulatory Physiology, 2001, 280(5): 2292-2299.
[5] Apostolova M D, Ivanova I A, Cherian M Q, et al. Metallothioneinand apoptosis during differentiation of myoblasts to myotubes: protection against free radieal toxieity[J]. Toxicology and Applied Pharmacology, 1999, 159(3): 175-184.
[6] Ye G, Metreveli N S, Ren J, et al. Metallothionein prevents diabetes-induced deficits in cardiomyocytes by inhibiting reactive oxygen species produetion[J]. Diabetes, 2003, 52(3): 777-783.
[7] Kang Y J, Li Y, Sun X, et al. Antiapoptotic effect and inhibition of ischemia/reperfusion-induced myocardial injury in metallothionein overexpressing transgenic mice[J]. American Journal of Pathology, 2003, 163(4): 1579-1586.
[8] Liang Q, Carlson E C, Donthi R V, et al. Overexpression of metallothionein reduces diabetic cardlomyopathy[J]. Diabetes, 2002, 51(1): 174-181.
[9] Min K S. Physiological significance of metallothionein in oxidative stress[J]. Yakugaku Zasshi, 2007, 127(4): 695-702.
[10] 张彬, 肖定福, 李丽立, 等. 金属硫蛋白在奶牛公犊体内的代谢规律研究[J]. 草业学报, 2008, 17(6): 78-84.
[11] 张彬, 薛立群, 李丽立, 等. 外源金属硫蛋白对奶牛抗热应激调控及SOD基因表达的影响[J]. 应用生态学报, 2007, 18(1): 193-198.
[12] Zhang B, Chen Y G, Wen G H, et al. Effects of metallothionein on the thermoresistance and SOD gene expression in dairy cattle[C]. The 5th International Conference on Metallothionein, Peking, 2005.
[13] 李丽立, 张彬, 印遇龙, 等. 猪肝金属硫蛋白的诱导[J]. 广西农业生物科学, 2004, 23(4): 270-273.
[14] 张彬, 吴力专, 李丽立. 一种猪金属硫蛋白的测定方法[P]. 中华人民共和国国家知识产权局(发明专利号ZL200410061265. 9), 2007.
[15] Aji J H R, Kojima Y. Chemistry and biochemistry of metallothionein[J]. Experimental Biology, 1987, 52: 25-61.
[16] Cail J, Klein B, Kang Y. Metallothionein inhibits peroxynitrite-induced DNA and lipoprotei damage[J]. Journal of Biological Chemistry, 2000, 275(50): 57-60.
[17] Ravindra N, Dinender K, Timao L, et al. Metallothioneins, oxidative stress and the cardiovascular system[J]. Toxicology, 2000, 155: 17-26.
[18] Baird S K, Kurz T, Brunk U T. Metallothionein protects against oxidative stress induced lysosomal destabilization[J]. Biochemistry, 2006, 394: 275-283.
[19] Riazantseva N V, Novitski V V, Chasovskikh N, et al. Redox-dependent regulation of apoptosis as a function of intracellular reactive oxygen species in oxidative stress[J]. Rossiǐskii FiziologicheskiǐZhurnal Imeni I.M. Sechenova/Rossiǐskaia Akademiia Nauk, 2008, 94(6): 710 -718.
[20] 刘云华, 张彬. 金属硫蛋白在养殖业的应用前景[J]. 家畜生态学报, 2004, 25(4): 179-182.
[21] 张勇峰, 殷波. 混合盐碱胁迫对苗期紫花苜蓿抗氧化酶活性及丙二醛含量的影响[J]. 草业学报, 2009, 18(1): 46-50.
[22] 苏桐, 龙瑞军, 魏小红, 等. 外源NO对NaCl胁迫下燕麦幼苗氧化损伤的保护作用[J]. 草业学报, 2008, 17(5): 48-53.
[23] Davis S R, Cousins R J. Metallothionein expression in animals: A physiological perspective on function[J]. Journal of Nutrition, 2000, 130: 1085-1088.
[24] Ravindra N, Dinender K, Timao L, et al. Metallothioneins, oxidative stress and the cardiovascular system[J]. Toxicology, 2000, 155: 17-26.
[25] Sato M, Saaski M, Hoio H, et al. Antioxdative roles of mellothionein and maganese superoxide disnutase induced by turner necrosis factor[J]. Biochemistry, 1999, 82: 70-77.
[26] 李丽立, 刘云华, 侯德兴, 等. 金属硫蛋白对仔猪抗氧化功能的影响[J]. 第四军医大学学报, 2006, 27(19): 1733-1736.
[27] Li L L, Hou Z P, Yin Y L, et al. Intramuscular administration of zinc metallothionein to preslaughter stressed pigs improves anti-oxidative status and pork quality[J]. Asian-Australia Journal of Animal Science, 2007, 20(5): 761-767.