微囊藻毒素(MC-RR)对白三叶种子萌发及幼苗生理生化特性影响

草业学报 ›› 2009, Vol. 18 ›› Issue (6): 180-185.

微囊藻毒素(MC-RR)对白三叶种子萌发及幼苗生理生化特性影响

薛延丰¹,李慧明^1,2,易能^1,3,李优琴¹,石志琦^1*

1.江苏省农业科学院食品质量安全检测研究所, 江苏南京 210094;
2.南京大学生命科学院, 江苏南京 210093;

3.南京农业大学资源与环境资源学院, 江苏南京 210095

收稿日期:2009-04-30 出版日期:2009-12-20 发布日期:2009-12-20
作者简介:薛延丰(1978-),男,河南孟州人,博士。E-mail: xueyanfeng@jaas.ac.cn
基金资助:
973预研项目(2008CB117001)资助

Effects of MC-RR on the seed germination and characteristics of physiologyand biochemistry in Trifolium repens seedlings

XUE Yan-feng¹,LI Hui-ming^1,2,YI Neng^1,3,LI You-qin¹,SHI Zhi-qi¹

1.Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China；

2.College of Life Science, Nanjing University, Nanjing 210093, China；
3.College of Resources
and Environmental Science, Nanjing Agriculture University, Nanjing 210095, China

Received:2009-04-30 Online:2009-12-20 Published:2009-12-20

摘要/Abstract

摘要： 以白三叶种子和幼苗为材料,研究了不同浓度MC-RR对白三叶种子萌发、抗氧化酶活性变化以及MC-RR在白三叶体内积累规律。结果表明,发芽率和发芽势随着处理浓度的增加而降低,在高浓度处理下,与对照相比显著降低;株高随着MC-RR处理浓度增加而显著降低,根长和鲜重在低浓度处理下显著增加,在高浓度处理下显著降低;抗氧化酶活性的变化不尽相同,SOD活性随着处理浓度的增加与对照相比呈先增加后降低而后又增加的趋势,POD活性随着处理浓度的增加呈先增加后降低的趋势,CAT活性的变化趋势不同于SOD和POD;单位鲜重白三叶MC-RR含量随着处理浓度的增加与0.05 mg/L处理下的MC-RR含量相比显著增加,而生物富集系数的变化趋势则与单位鲜重白三叶MC-RR含量相反,随着MC-RR处理浓度的增加而降低。说明随着MC-RR处理浓度的增加,白三叶受到的伤害增大。

Abstract: The seed and seedlings of Trifolium repens were treated with various concentrations of MC-RR. The effects of MC-RR on the seed germination, the change of antioxidant enzymes and the regulation of MC-RR accumulation in T. repens were studied in this paper. The results showed that the germination percentage and the germinating viability decreased with the increase of MC-RR concentration. When at the higher concentration, the germination percentage and the germinating viability significantly decreased compared with the control. Shoot height markedly inhibited with the rising of MC-RR concentration. Root length and fresh weight significantly increased under the lower concentration, whereas they markedly reduced at the higher concentration. The changes of antioxidant enzymes were different each other. SOD activity indicated the trend of increase-decrease-increase compared with the control. POD activity illustrated that it increased firstly and then decreased. However, the trend of CAT activity was different from SOD and POD. The MC-RR content of T. repens increased with the treating concentration compared with the concentration of 0.05 mg/L, whereas bioconcentration factor (BCF) decreased. All the results suggested that the seed and seedlings of T. repens would be damaged seriously with the increase of MC-RR concentration.

中图分类号:

薛延丰,李慧明,易能,李优琴,石志琦. 微囊藻毒素(MC-RR)对白三叶种子萌发及幼苗生理生化特性影响[J]. 草业学报, 2009, 18(6): 180-185.

XUE Yan-feng,LI Hui-ming,YI Neng,LI You-qin,SHI Zhi-qi. Effects of MC-RR on the seed germination and characteristics of physiologyand biochemistry in Trifolium repens seedlings[J]. Acta Prataculturae Sinica, 2009, 18(6): 180-185.

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