DREB/CBF类转录因子研究进展及其在草坪草和牧草抗逆基因工程中的应用

摘要/Abstract

摘要： 草坪草和牧草是可持续农业的重要组成部分,但它们的生存力、生物量及产量的增长往往受限于各种逆境胁迫因素。作为受多基因控制的数量性状,抗逆性分子基础的研究显得尤为重要。转录因子调控着与逆境相关的多个下游靶基因的表达。其中,DREB/CBF类转录因子能与脱水应答顺式作用元件结合,由DREB1/CBF和DREB2两个亚家族成员组成。前者受低温诱导,后者受干旱诱导。近年来草坪草和牧草的遗传转化技术突飞猛进,通过转化DREB/CBF类转录因子来改良草种的抗逆性是今后草业育种工作的一个重要发展方向。本研究综述了该类转录因子的结构特征及其在植物逆境信号转导中的作用,并对它们在草坪草和牧草抗逆基因工程中的应用作了相关介绍。

Abstract: Turf and forage grasses are critical to sustainable agriculture, but stresses negatively influence their survival, biomass and yield. It is essential to understand the molecular basis of stress tolerance since it is multigenic as well as a quantitative trait. Transcription factors that regulate expression of several genes related to stress have been discovered. One such class is DREB/CBF that binds to drought responsive cis-acting elements. DREBs belong to the ERF family of transcription factors consisting of two subclasses, i.e. DREB1/CBF and DREB2 that are induced by cold and dehydration, respectively. Tremendous progress had been made in genetic transformation of turf and forage grasses in the past decade. The rapid advancement of molecular biology and transgenic technology provides novel methods to accelerate and complement conventional breeding efforts. It is possible to engineer stress tolerance in transgenic plants by manipulating the expression of DREBs. This opens an excellent opportunity to develop stress tolerant grasses in future. This review intends to focus on the structure, role of DREBs in plant stress signaling and the present status of their deployment in developing stress tolerant transgenic turf and forage grasses.

中图分类号:

王舟,刘建秀. DREB/CBF类转录因子研究进展及其在草坪草和牧草抗逆基因工程中的应用[J]. 草业学报, 2011, 20(1): 222-236.

WANG Zhou, LIU Jian-xiu. Advances in studies on DREB/CBF transcription factors, and their applications
in genetic engineering for stress tolerance of turf and forage grasses[J]. Acta Prataculturae Sinica, 2011, 20(1): 222-236.

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