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草业学报 ›› 2012, Vol. 21 ›› Issue (1): 213-219.

• 研究论文 • 上一篇    下一篇

水杨酸对盐胁迫下花椰菜种子萌发及幼苗生理特性的影响

王玉萍1,董雯1,张鑫1,杨茜1,张峰1,2*   

  1. 1.甘肃农业大学农学院 甘肃省干旱生境作物学重点实验室 甘肃省作物遗传改良与种质创新重点实验室,甘肃 兰州 730070;
    2.甘肃农业大学生命科学技术学院,甘肃 兰州 730070
  • 出版日期:2012-02-20 发布日期:2012-02-20
  • 通讯作者: E-mail:zhangf@gsau.edu.cn
  • 作者简介:王玉萍(1975-),女,甘肃天水人,副教授,博士。
  • 基金资助:
    甘肃省自然科学基金项目(0803RJZA051)和国家自然科学基金项目(31060063)资助。

Effects of salicylic acid on seed germination and physiological characters of cauliflower seedlings under salt stress

WANG Yu-ping1, DONG Wen1, ZHANG Xin1, YANG Qian1, ZHANG Feng1,2   

  1. 1.College of Agronomy, Gansu Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, China;
    2.College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
  • Online:2012-02-20 Published:2012-02-20

摘要: 以先华70和祁连白雪2个耐盐性不同的花椰菜品种为材料,在125 mmol/L NaCl胁迫处理下,研究了不同浓度水杨酸对花椰菜种子萌发及幼苗生理特性的影响。结果表明,盐胁迫下,0.5~1.5 mmol/L SA浸种预处理的花椰菜种子具有较高的发芽率、发芽势、发芽指数及ɑ-淀粉酶活性,其中以1.0 mmol/L的SA处理效果最好;0.5~1.5 mmol/L SA处理的花椰菜幼苗具有较高的株高、茎粗和较大的叶面积,其叶绿素含量、可溶性糖含量、脯氨酸含量以及超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性均较高,而丙二醛(MDA)含量较低,其中以1.0 mmol/L SA处理效果最好。说明0.5~1.5 mmol/L外源SA可以通过提高ɑ-淀粉酶活性,提高花椰菜幼苗渗透调节能力和抗氧化能力,诱导花椰菜种子萌发和幼苗的耐盐性,减轻和缓解盐害,SA浓度超过2.0 mmol/L时对盐胁迫没有缓解效应。

Abstract: Effects of salicylic acid on salt stress-induced injuries were investigated during the stage of seed germination and seedling growth of cauliflower (Brassica oleracea var. botrytis). Under the stress, presoaking the seeds with 0.5-1.5 mmol/L of salicylic acid increased the germination percentage, germination energy, germination index and relative α-amylase activity of cauliflower. Among the treatment of salicylic acid, 1.0 mmol/L had the best effect. The results also showed that treatment with 0.5-1.5 mmol/L of salicylic acid during the stage of seedling growth under salt stress increased the plant height, stem diameter, leaf area, chlorophyll contents, free proline and water dissolved-carbohydrate contents, the activities of superoxide dismutase (SOD) and peroxidase (POD), and decreased the content of malondialdehyde (MDA), suggesting that the exogenous SA (salicylic acid) could effectively mitigate the injuries of salt stress and increase salt-tolerance of cauliflower seeds and seedlings by increasing α-amylase activity, osmotic regulation and antioxidation. The best treatment concentration of salicylic acid was 1.0 mmol/L. When the concentration of SA was beyond 2.0 mmol/L, no mitigation effect was observed on the seeds germination and growth of seedling under salt stress.

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