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草业学报 ›› 2015, Vol. 24 ›› Issue (9): 80-88.DOI: 10.11686/cyxb2014426

• 研究论文 • 上一篇    下一篇

松嫩平原盐碱化羊草群落中AM真菌物种资源及侵染率研究

张义飞1,*,毕琪2,杨允菲3,张忠辉1,胡长群1,杨雨春1,赵珊珊1,王相刚4   

  1. 1.吉林省林业科学研究院,吉林 长春 130033;
    2.东北师大附属中学,吉林 长春 130022;
    3.东北师范大学生命科学学院,吉林 长春 130024;
    4.敦化市明星特产科技开发有限公司,吉林 敦化133000
  • 收稿日期:2014-10-13 出版日期:2015-09-20 发布日期:2015-09-20
  • 通讯作者: E-mail:yifeii@hotmail.com
  • 作者简介:张义飞(1972-),男,吉林长春人,助理研究员,博士。E-mail:yifeii@hotmail.com
  • 基金资助:
    国家自然科学基金资助项目(30270260,30770397),林业公益性行业科研专项(201104040)和吉林省科技发展计划(201205065)资助

Arbuscular mycorrhizal fungi diversity in saline-alkaline Leymus chinensis grasslands on the Songnen Plain

ZHANG Yi-Fei1, *, BI Qi2, YANG Yun-Fei3, ZHANG Zhong-Hui1, HU Chang-Qun1, YANG Yu-Chun1, ZHAO Shan-Shan1, WANG Xiang-Gang4   

  1. 1.Jilin Academy of Forestry Science, Changchun 130033, China;
    2.High School Attached to Northeast Normal University, Changchun 130022, China;
    3.School of Life Science, Northeast Normal University, Changchun 130024, China;
    4.Dunhua Star Local Products Technology Development Co. Led., Dunhua 133000, China
  • Received:2014-10-13 Online:2015-09-20 Published:2015-09-20

摘要: 本研究在松嫩平原西部12个地区15个重度盐碱化草地中,调查了羊草群落天然斑块中AM真菌的种类和分布、AM真菌羊草根系的侵染能力及土壤pH值的影响。共分离出AM真菌4属11种,其中球囊霉属(Glomus)占总物种数的72.42%,在各调查样点中出现频度最高,其中摩西球囊霉(G. mossea)出现频度达100.0%。土壤pH强烈抑制盐碱化草地中羊草天然群落斑块中AM真菌的物种丰富度,但对孢子密度的影响未达到显著水平。AM真菌对羊草根系的侵染频率和侵染强度显著正相关。AM真菌对羊草根系的侵染频率和侵染强度随着AM物种数量的增加而增强,随着土壤pH的增加而下降。在盐碱化羊草地中存在较丰富的侵染羊草根系的AM真菌资源,研究结果为筛选和利用耐盐碱AM真菌菌株以恢复和重建松嫩盐碱化羊草草地生态系统提供了理论依据。

Abstract: Arbuscular mycorrhizal fungi (AMF) are beneficial microorganisms, distributed widely in many different soil types. The investigation of species diversity of AMF in extreme environments is a rapidly developing area of research because of the potential benefits for ecosystem restoration. Screening for effective arbuscular mycorrhizal fungi species is regarded as an important approach to successful revegetation. The identification of arbuscular mycorrhizal species and their distribution were investigated in 15 natural saline-alkaline Leymus chinensis grasslands in 12 regions of the western Songnen Plain. The occurrence frequency, species richness and spore density were also investigated. The ability of AMF to infect roots of L. chinensis, including colonization rate and infection intensity, and the effect of soil pH were measured. In total, 11 species from 4 different families were identified; 72.4% of species belonged to Glomus. One species, G. mossea, was found at all sites. High soil pH strongly decreased AMF species richness in natural saline-alkaline communities of L. chinensis, but did not affect spore density. Disturbance of soil through erosion may be an important factor influencing spore density in soil because AMF spores were seldom detected in bare soil where the surface had been significantly disturbed. Root colonization rate was positively correlated with infection intensity. The infection of L. chinensis roots was increased with increasing AMF species richness, depressed by increasing soil pH. Our research indicated that there was an abundance of AMF species in saline-alkaline grassland able to infect roots of L. chinensis, and suggested approaches for screening saline-alkaline tolerant AMF species with the potential to help restore the degraded grassland ecosystem on the Songnen Plain.