农杆菌介导含硫氨基酸γ-zein转化菊苣的初步研究

doi:10.11686/cyxb2014307

草业学报 ›› 2015, Vol. 24 ›› Issue (9): 73-79.DOI: 10.11686/cyxb2014307

农杆菌介导含硫氨基酸γ-zein转化菊苣的初步研究

张玉^1,*,白史且¹,李聪²

1.四川省草原科学研究院,四川成都611731;
2.中国农业科学院北京畜牧兽医研究所,北京 100094

收稿日期:2014-07-07 出版日期:2015-09-20 发布日期:2015-09-20
作者简介:张玉(1975-),女,四川仁寿人,博士。E-mail:zhyforage@126.com
基金资助:
四川省十二五牧草育种攻关(2011NZ0098-11),四川省应用基础项目(2013JY0111)和国家牧草产业技术体系阿坝综合试验站资助

Preliminary studies on transgenic chicory using the sulphur-amino acid gene, γ-zein, mediated by Agrobacterium tumefacien

ZHANG Yu^{1, *}, BAI Shi-Qie¹, LI Cong²

1.Sichuan Grassland Science Academ, Chengdu 611731, China;
2.Institute of Animal Science,Chinese Academy of Agricultural Science, Beijing 100094, China

Received:2014-07-07 Online:2015-09-20 Published:2015-09-20

摘要/Abstract

摘要： 含硫氨基酸具有动物营养与免疫相关的重要生理功能,为提高菊苣中含硫氨基酸含量,采用根癌农杆菌介导法将玉米种子贮藏蛋白含硫氨基酸基因γ-zein和绿色荧光蛋白GFP融合基因转入到菊苣无菌苗叶片中,经过共培养、潮霉素抗性筛选、分化、再生和炼苗, 得到抗性植株。对抗性植株进行PCR、PCR-Southern、斑点杂交和RT-PCR分析,结果表明,外源目的基因已经整合到菊苣基因组中并且得到了表达,为提高菊苣含硫氨基酸含量,改善其品质奠定了基础。

Abstract: Sulfur-containing amino acids have important physiological functions related to animal nutrition and immunity. To improve the sulfur-amino acid content of chicory, leaves of chicory were transformed with the Sulphur-amino acid gene γ-zein, an important prolamin storage protein from Zea mays and a green fluorescent protein (GFP) gene using Agrobacterium mediated transfusion. After co-culture, selective differentiation and regeneration, hygromycin resistant plants were obtained. Resistant plants were detected using PCR, PCR-southern, dot blot hybridization and RT-PCR. The results demonstrated that the γ-zein genes had been integrated into the genome of chicory and expressed on a nucleic acid level in the transgenic plants.

张玉,白史且,李聪. 农杆菌介导含硫氨基酸γ-zein转化菊苣的初步研究[J]. 草业学报, 2015, 24(9): 73-79.

ZHANG Yu, BAI Shi-Qie, LI Cong. Preliminary studies on transgenic chicory using the sulphur-amino acid gene, γ-zein, mediated by Agrobacterium tumefacien[J]. Acta Prataculturae Sinica, 2015, 24(9): 73-79.

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农杆菌介导含硫氨基酸γ-zein转化菊苣的初步研究

Preliminary studies on transgenic chicory using the sulphur-amino acid gene, γ-zein, mediated by Agrobacterium tumefacien

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