Abstract: Bermudagrass (Cynodon dactylon) is a perennial, warm-season turfgrass well known and widely used for different purposes throughout the world. In order to further clarify the characteristics and genetic basis of C. dactylon germplasm in China, this study performed a comparative study of the phenotypic traits and molecular genetics of 5 cultivars and 1 breeding line, as well as 1 cultivar introduced in China. The green stage and turf speed are 53 and 269 d for the breeding line '02011', higher than the 5 cultivars. The differences between cultivars in terms of plant height, flag leaf, creeping stem, and inflorescence characteristics were highly significant (P<0.01). 8 DUS (distinctness, uniformity and stability) characteristics (including stolon surface color, degrees of heading, color of surface blade, reproductive shoot cyanine glucoside color, growth habit, glume cyanine glucoside color, glume tip cyanine glucoside color) differed among the tested cultivars to varying degrees. 167 and 316 bands were amplified for 18 pairs of primers by SRAP and SSR, of which 87.51% and 70.13% were polymorphic. SRAP and SSR showed that C. dactylon cultivars from the same geographical area could exhibit similar phenotypic traits and relatively close genetic similarities.