我国北方9份旱生-沙生植物蒙古冰草遗传多样性研究

doi:10.11686/cyxb2015219

草业学报 ›› 2016, Vol. 25 ›› Issue (3): 77-85.DOI: 10.11686/cyxb2015219

我国北方9份旱生-沙生植物蒙古冰草遗传多样性研究

李晓全^{1, 2}, 高有汉¹, 刘扬¹, 索培芬¹, 韩冰^{1, 2, *}

1.内蒙古农业大学生命科学学院,内蒙古呼和浩特 010010;
2.中国农业科学院草原研究所,内蒙古呼和浩特 010010

收稿日期:2015-04-24 出版日期:2016-03-20 发布日期:2016-03-20
通讯作者: E-mail:hb_nmg@163.com
作者简介:李晓全(1991-),男,河北衡水人,在读硕士.E-mail:1206966950@qq.com
基金资助:
国家自然科学基金(31060057),中国科学院西部之光"人才培养"项目匹配经费和中国农业科学院草原研究所农业科技创新工程资助

The genetic diversity of 9 populations of dry-desert Agropyron mongolicum collected in northern China

LI Xiao-Quan^{1, 2}, GAO You-Han¹, LIU Yang¹, SUO Pei-Fen¹, Han Bing^{1, 2, *}

1.College of Life Sciences Inner Mongolia Agricultural University, Hohhot 010010, China;
2.Grassland Research Institute of Chinese Academy of Agricultural Sciences, Hohhot 010010, China

Received:2015-04-24 Online:2016-03-20 Published:2016-03-20

摘要/Abstract

摘要： 蒙古冰草因其抗寒,抗旱特性,是欧亚大陆荒漠草原优势植物之一.本文从染色体倍性及SSR序列长度多态性两个方面对采自中国北方境内的9个蒙古冰草居群进行遗传多样性分析,发现9个蒙古冰草居群染色体基数为7,均为二倍体,在染色体倍性方面不具有多态性;共采用138对小麦SSR引物进行扩增分析,共有21对引物扩增出特异性条带,SSR引物筛选率15.2%.共扩增出特异性条带119条,平均每对引物扩增出特异性条带5.6条,SSR序列长度多态性丰富.利用POPGEN 32软件计算9个蒙古冰草居群遗传多样性指标,居群P₈遗传多样性程度最低,居群P₃最高.AMOVA分析显示,蒙古冰草的遗传差异主要是来自居群内个体之间.UPGMA方法聚类分析,在遗传相似系数为0.80时,9个居群被分为三大类,居群P₁~P₆一类,居群P₇,P₈为第二类,P₉被单独分为一类.本研究为了解蒙古冰草遗传背景及加速其资源的合理开发利用奠定了理论基础.

Abstract: Agropyron mongolicum is one of the dominant species in desert steppe across Eurasia, due primarily to its cold and drought resistance. In this study, we analyzed chromosome polymorphism and DNA polymorphism in 9 populations of A. mongolicum in northern China. The chromosome number of the 9 populations was 7. Cells were diploid and showed no polymorphism in chromosome ploidy. A total of 138 pairs of wheat SSR primers were amplified and analyzed. A total of 21 primer pairs were amplified with specific fragments. The screening rate of SSR primers was 15.2%. A total of 119 specific bands were amplified: specificity was 5.6 and the polymorphism of DNA was rich. POPGEN 32 software was used to calculate the genetic diversity of the 9 populations of A. mongolicum. Population P₈ was found to have the least level of diversity, while P₃ had the highest. AMOVA software was used to analyze genetic differentiation, indicating that genetic differences come mainly from individuals in the populations. The UPGMA method was used for a cluster analysis of the 9 populations. When the genetic similarity coefficient is 0.80, the materials tested divided into three groups: P₁-P₆, P₇-P₈ and P₉. This paper lays the foundation for the development and utilization of new varieties of A. mongolicum.

李晓全, 高有汉, 刘扬, 索培芬, 韩冰. 我国北方9份旱生-沙生植物蒙古冰草遗传多样性研究[J]. 草业学报, 2016, 25(3): 77-85.

LI Xiao-Quan, GAO You-Han, LIU Yang, SUO Pei-Fen, Han Bing. The genetic diversity of 9 populations of dry-desert Agropyron mongolicum collected in northern China[J]. Acta Prataculturae Sinica, 2016, 25(3): 77-85.

参考文献

[1] Yun J F, Mi F G. The species and distribution of Agropyron gaertn grass. Chinese Journal of Grassland, 1989, (3): 14-17.
[2] Yun J F, Mi F G. Agropyron mongolicum Keng: a good grass in the drought region. Grassland and Prataculture, 1990, (2): 70-71.
[3] An Y, Wang Y Q, Chen M, et al . Research of the interseeding technique and effects of the practice on sandy pasture. Journal of Grassland, 1997, (1): 33-41.
[4] Li L H, Yang X M, Li X Q, et al . Introduction of desirable genes from Agropyron cristatum into common wheat by intergeneric hybridization.Scientia Agricultura Sinica, 1998, 31(6): 1-5.
[5] Jensen K B. Cytogenetics of Elymus magellanicus and its intra-generic and inter-generic hybrids with Pseudoroegneria spicata , Hordeum violaceum , Elymus trachycaulus , E. lanceolatus , and E. glaucus (Poaceae, Triticeae). Genome, 1993, 36(1): 72-76.
[6] Yang Y, Liu D J. Production, morphology and cytogenetics of intergeneric hybrids of Elymus species with Triticum aestivum L. and their backcross derivatives.Genome, 1987, 29(5): 689-694.
[7] Li J X. Genetic Diversity of Germplasm Resources of Agropyron cristatum in Inner Mongolia[D]. Hohhot: Inner Mongolia Agriculture University, 2005.
[8] Xie X M, Yun J F, Zhao B, et al . Allozyme analysis of genetic diversity of Agropyron mongolicum . Pratacultural Science, 2001, 18(6): 6-11.
[9] Lan B X, Li L H, Wang H. Genetic diversity of Agropyron mongolicum Keng populations. Scientia Agricultura Sinica, 2005, 38(3): 468-473.
[10] Bao M L, Yu Z, Ma Y H, et al . ISSR analysis on chromosome doubling plants F 2 of reciprocal hybrids between Agropyron mongolicum and A. cristatum cv. Fairway. Chinese Journal of Grassland, 2010, 32(5): 31-35.
[11] Han B, Zhao M L, Zhang H M, et al . Study on extraction method of genome DNA in Stipa . Journal of Inner Mongolia Agricultural University, 2002, (4): 32-35.
[12] Li T J, Shi J Y, Zhang X W. The preparation method of periwinkle leaf cell suspension.Research and Practice of Chinese Medicines, 2009, (4): 31-32.
[13] Gui Y J, Wang C, Quan L Y. Comparative analysis of Phyllostachys heterocycla (Carr.) Mitford cv. pubescens genome size and sequence. Science in China C: Life Science, 2007, 37(4): 488-492.
[14] Roder M S, Korzun V, Wendehake K, et al . A microsatellite map of wheat.Genetics, 1998, 149(4): 2007-2023.
[15] Panaud O, Chen X, McCouch S R. Development of a microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice ( Oryza sativa L.). Molecular and General Genetics, 1996, 252: 597-607.
[16] Tang Q L. Identification of Genome Components Among Species of Genus Zea and Introgression of Maize Relatives for Maize Improvement[D]. Yaan: Sichuan Agriculture University, 2003.
[17] Khush G. Cytogenetics of Aneuploids[M]. New York: Academic Press, 1973.
[18] Shan D, Zhao M L, Yun J F, et al . Preliminary study on application of Stipa krylovii and Agropyron cristatum by SSR primers derived from wheat. Grassland of China, 2005, (4): 20-34.
[19] Yu Z, Xie R, Yu X X, et al . SSR analysis of sorghum-sudangrass new strains with low hydrocyanic acid content. Acta Prataculturae Sinica, 2014, 23(1): 223-228.
[20] 云锦凤, 米福贵. 冰草属牧草的种类与分布. 中国草地学报, 1989, (3): 14-17.
[21] 云锦凤, 米福贵. 干旱地区一种优良牧草蒙古冰草. 草原与草业, 1990, (2): 70-71.
[22] 安渊, 王育青, 陈敏, 等. 沙地草场补播技术及其生态效益研究.草地学报, 1997, (1): 33-41.
[23] 李立会, 杨欣明, 李秀全, 等. 通过属间杂交向小麦转移冰草优异基因的研究. 中国农业科学, 1998, 31(6): 1-5.
[24] 李景欣. 内蒙古冰草种质资源遗传多样性研究[D]. 呼和浩特: 内蒙古农业大学, 2005.
[25] 解新明, 云锦凤, 赵冰, 等. 蒙古冰草遗传多样性的等位酶分析. 草业科学, 2001, 18(6): 6-11.
[26] 兰保祥, 李立会, 王辉. 蒙古冰草居群遗传多样性研究. 中国农业科学, 2005, 38(3): 468-473.
[27] 包美莲, 于卓, 马艳红, 等. 蒙古冰草与航道冰草正反交杂种染色体加倍植株 F 2 的 ISSR分析. 中国草地学报, 2010, 32(5): 31-35.
[28] 韩冰, 赵萌莉, 张红梅, 等. 针茅属植物基因组DNA提取方法的研究. 内蒙古农业大学学报, 2002, (4): 32-35.
[29] 李铁军, 石俊英, 张孝卫. 长春花叶片细胞核悬液的简便制备方法. 现代中药研究与实践, 2009, (4): 31-32.
[30] 桂毅杰, 王晟, 全丽艳. 毛竹基因组大小和序列构成的比较分析. 中国科学 C 辑: 生命科学, 2007, 37(4): 488-492.
[31] 唐祈林. 玉蜀黍属基因组构成及创制玉米新种质研究[D]. 雅安: 四川农业大学, 2003.
[32] 珊丹, 赵萌莉, 云锦凤, 等. 小麦微卫星引物在克氏针茅和冰草中应用的初步研究. 中国草地, 2005, (4): 20-34.
[33] 于卓, 谢锐, 于肖夏, 等. 低氢氰酸含量高丹草新品系及其亲本的SSR分析. 草业学报, 2014, 23(1): 223-228.

我国北方9份旱生-沙生植物蒙古冰草遗传多样性研究

The genetic diversity of 9 populations of dry-desert Agropyron mongolicum collected in northern China

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics